CD45RA Antibody (MIL13)

Immunohistochemistry-Paraffin: CD45RA Antibody (MIL13) [NB100-64997] - Porcine skin section was stained with anti-CD45RA antibody. Image from verified customer review.

Flow Cytometry: CD45RA Antibody (MIL13) [NB100-64997] - Analysis of porcine peripeheral blood lymphocytes with antibody followed by Goat anti Mouse IgG:RPE

Product Discontinued

CD45RA Antibody (MIL13) Summary

• Immunogen: Cells isolated from porcine mesenteric lymph node
• Specificity: This antibody recognizes an epitope contained in the portion of porcine CD45 encoded by exon A, CD45RA. Antibody recognizes both the 210kDa RA CD45 isoform and the 226kDa RAC isoform. Antibody does not recognize the CD45RC or CD45RO isoforms.
• Isotype: IgG1
• Clonality: Monoclonal
• Host: Mouse
• Gene: PTPRC
• Purity: Tissue culture supernatant

Applications/Dilutions

• Dilutions:
  • Flow Cytometry Neat
  • Immunohistochemistry 1:10-1:500
  • Immunohistochemistry-Frozen 1:10-1:500
  • Immunohistochemistry-Paraffin 1:10-1:500

Packaging, Storage & Formulations

• Storage: Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
• Buffer: PBS
• Preservative: 0.09% Sodium Azide
• Purity: Tissue culture supernatant

Alternate Names for CD45RA Antibody (MIL13)

• B220
• CD45 antigen
• CD45R
• CD45RA
• EC 3.1.3.48
• L-CA
• LY5
• protein tyrosine phosphatase, receptor type, C
• receptor-type tyrosine-protein phosphatase C
• T200 glycoprotein
• T200 leukocyte common antigen
• T200receptor type, c polypeptide

Background

CD45 is a family of single chain transmembraneous glycoproteins consisting of at least four isoforms (220, 205, 190, 180 kDa) which share a common large intracellular domain. Their extracellular domains are heavily glycosylated. The different isoforms are produced by alternative messenger RNA splicing of three exons of a single gene on chromosome 1. CD45 is expressed on cells of the human hematopoietic lineage (including hematopoietic stem cells) with the exception of mature red cells. It is not detected on differentiated cells of other tissues. It is likely that CD45 plays an important role in signal transduction, inhibition or upregulation of various immunological functions. Antibodies recognizing a common epitope on all of the isoforms are termed CD45 whilst those recognizing only individual isoforms are termed CD45RA or CD45RO etc.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Review for CD45RA Antibody (NB100-64997) (1)

Average Rating: 5

(Based on 1 review)

We have 1 review tested in 1 species: Other.

reviewed by: Jesse Wu IHC-P Other 10/16/2014

Summary

• Application: Immunohistochemistry-Paraffin
• Sample Tested: Pig Skin
• Species: Other

Bioinformatics

• Gene Symbol: PTPRC