CD45RA Antibody (1069433) [Phycoerythrin]

PBMC lymphocytes were stained with Mouse Anti-Human CD45RO Alexa Fluor® 647‑conjugated Monoclonal Antibody (Catalog # FAB10642R) and either (A) Mouse Anti-Human CD45RA PE‑conjugated Monoclonal Antibody (Catalog # FAB11444P) or (B) isotype control antibody (Catalog # IC003P). View our protocol for Staining Membrane-associated Proteins.

• Reactivity: Hu
• Applications: Flow
• Clone: 1069433
• Clonality: Monoclonal
• Host: Mouse
• Conjugate: Phycoerythrin

CD45RA Antibody (1069433) [Phycoerythrin] Summary

• Immunogen: Whole cell transfectant expressing CD45RA
  Accession # P08575
• Specificity: Detects CD45RA in direct ELISA.
• Source: N/A
• Isotype: IgG1
• Clonality: Monoclonal
• Host: Mouse
• Purity Statement: Protein A or G purified from hybridoma culture supernatant

Applications/Dilutions

• Dilutions:
  • Flow Cytometry 10 uL/10^6 cells

Packaging, Storage & Formulations

• Storage: Protect from light. Do not freeze.
  • 12 months from date of receipt, 2 to 8 °C as supplied.
• Buffer: Supplied in a saline solution containing Sodium Phosphate and Sodium Azide.

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for CD45RA Antibody (1069433) [Phycoerythrin]

• B220
• CD45 antigen
• CD45R
• CD45RA
• EC 3.1.3.48
• L-CA
• LY5
• protein tyrosine phosphatase, receptor type, C
• receptor-type tyrosine-protein phosphatase C
• T200 glycoprotein
• T200 leukocyte common antigen
• T200receptor type, c polypeptide

Background

This monoclonal antibody specifically binds to the 220 kDa isoform of human CD45, CD45RA.  CD45RA is a single chain type I glycoprotein that is encoded by the human PTPRC gene.  In humans, CD45RA is one of six isoforms of the CD45 complex.  These different isoforms are the result of alternative splicing and some of the other isoforms include CD45RO, CD45RB, and CD45RC.  CD45RO, CD45RB, CD45RC, and CD45RA are primarily expressed on T cells.  CD45RA contains exon 4 but lacks both exons 5 and 6.  CD45RO contains exons 3, 7, and 8 but not RA, RB, and RC exons.  Phenotypically, CD45RA is highly expressed on naïve T cells whereas CD45RO is expressed on activated and memory T cells.  Other cell types such as activated monocytes and B cells can also express CD45RO. Both CD45RA and CD45RO are used to characterize different subsets of Memory T cells.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Bioinformatics

• Uniprot:
  • P08575()