CD45RA Antibody (1069433) [mFluor Violet 450 SE]

PBMC lymphocytes were stained with Mouse Anti-Human CD45RO PE‑conjugated Monoclonal Antibody (Catalog # FAB10642P) and either (A) Mouse Anti-Human CD45RA mFluor™ Violet 450-Conjugated Monoclonal Antibody (Catalog # FAB11444MFV450) or (B) unstained . View our protocol for Staining Membrane-associated Proteins.

• Reactivity: Hu
• Applications: Flow
• Clone: 1069433
• Clonality: Monoclonal
• Host: Mouse
• Conjugate: mFluor Violet 450 SE

CD45RA Antibody (1069433) [mFluor Violet 450 SE] Summary

• Immunogen: Whole cells (naïve T cells)
  Accession # P08575
• Specificity: Detects CD45RA in direct ELISA.
• Source: N/A
• Isotype: IgG2a
• Clonality: Monoclonal
• Host: Mouse
• Purity Statement: Protein A or G purified from hybridoma culture supernatant

Applications/Dilutions

• Dilutions:
  • Flow Cytometry 5 uL/10^6 cells

Packaging, Storage & Formulations

• Storage: Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C, as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after opening.
  • 6 months, -20 to -70 °C under sterile conditions after opening.
• Buffer: Supplied in a saline solution containing BSA and Sodium Azide. See Certificate of Analysis for details.
  *Contains <0.1% Sodium Azide, which is not hazardous at this concentration according to GHS classifications. Refer to the Safety Data Sheet(SDS) for additional information and handling instructions.

Notes

mFluor^TM is a trademark of AAT Bioquest, Inc.

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for CD45RA Antibody (1069433) [mFluor Violet 450 SE]

• B220
• CD45 antigen
• CD45R
• CD45RA
• EC 3.1.3.48
• L-CA
• LY5
• protein tyrosine phosphatase, receptor type, C
• receptor-type tyrosine-protein phosphatase C
• T200 glycoprotein
• T200 leukocyte common antigen
• T200receptor type, c polypeptide

Background

This monoclonal antibody specifically binds to the 220 kDa isoform of human CD45, CD45RA.  CD45RA is a single chain type I glycoprotein that is encoded by the human PTPRC gene.  In humans, CD45RA is one of six isoforms of the CD45 complex.  These different isoforms are the result of alternative splicing and some of the other isoforms include CD45RO, CD45RB, and CD45RC.  CD45RO, CD45RB, CD45RC, and CD45RA are primarily expressed on T cells.  CD45RA contains exon 4 but lacks both exons 5 and 6.  CD45RO contains exons 3, 7, and 8 but not RA, RB, and RC exons.  Phenotypically, CD45RA is highly expressed on naïve T cells whereas CD45RO is expressed on activated and memory T cells.  Other cell types such as activated monocytes and B cells can also express CD45RO.  Both CD45RA and CD45RO are used to characterize different subsets of Memory T cells.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Bioinformatics

• Uniprot:
  • P08575()