CD43/Sialophorin Antibody (SPN/839)

Images

 
Western Blot: CD43/Sialophorin Antibody (SPN/839) [NBP2-44648] - Western Blot Analysis of K562 cell lysate using CD43/Sialophorin Antibody (SPN/839).
Immunocytochemistry/ Immunofluorescence: CD43/Sialophorin Antibody (SPN/839) [NBP2-44648] - Immunofluorescence Analysis of K562 cells labeling CD43 with CD43/Sialophorin Antibody (SPN/839) followed by Goat anti-Mouse ...read more
Immunohistochemistry-Paraffin: CD43/Sialophorin Antibody (SPN/839) [NBP2-44648] - Human Spleen stained with CD43 Monoclonal Antibody (SPN/839).
Flow Cytometry: CD43/Sialophorin Antibody (SPN/839) [NBP2-44648] - Flow Cytometric Analysis of K562 cells using CD43/Sialophorin Antibody (SPN/839) followed by goat anti-Mouse IgG-CF488 (Blue); Isotype Control (Red).
Western Blot: CD43/Sialophorin Antibody (SPN/839) [NBP2-44648] - Analysis of human Spleen Lysate using CD43 Monoclonal Antibody (SPN/839).

Product Details

Summary
Reactivity HuSpecies Glossary
Applications WB, Flow, ICC/IF, IHC, IHC-P
Clone
SPN/839
Clonality
Monoclonal
Host
Mouse
Conjugate
Unconjugated
Concentration
0.2 mg/ml

Order Details

CD43/Sialophorin Antibody (SPN/839) Summary

Immunogen
Recombinant full-length human CD43/Sialophorin protein (Uniprot: P16150)
Localization
Cell surface
Marker
T-Cell Marker
Specificity
It recognizes a cell surface glycoprotein of 95/115/135kDa (depending upon the extent of glycosylation), identified as CD43. 70-90% of T-cell lymphomas and from 22-37% of B-cell lymphomas express CD43. No reactivity has been observed with reactive B-cells. So a B-lineage population that co-expresses CD43 is highly likely to be a malignant lymphoma, especially a low-grade lymphoma, rather than a reactive B-cell population. When CD43 antibody is used in combination with anti-CD20, effective immunophenotyping of the lymphomas in formalin-fixed tissues can be obtained. Co-staining of a lymphoid infiltrate with anti-CD20 and anti-CD43 argues against a reactive process and favors a diagnosis of lymphoma.
Isotype
IgG1 Kappa
Clonality
Monoclonal
Host
Mouse
Gene
SPN
Purity
Protein A or G purified
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.

Applications/Dilutions

Dilutions
  • Flow Cytometry 1-2 ug/million cells
  • Immunocytochemistry/Immunofluorescence 1-2 ug/ml
  • Immunohistochemistry
  • Immunohistochemistry-Paraffin 1-2 ug/ml
  • Western Blot 1-2 ug/ml
Application Notes
Immunohistochemistry (Formalin-fixed): 1-2ug/ml for 30 minutes at RT. Staining of formalin-fixed tissues requires heating tissue sections in 10mM Tris with 1mM EDTA, pH 9.0, for 45 min at 95C followed by cooling at RT for 20 minutes.
Optimal dilution for a specific application should be determined.

Packaging, Storage & Formulations

Storage
Store at 4C.
Buffer
10 mM PBS with 0.05% BSA
Preservative
0.05% Sodium Azide
Concentration
0.2 mg/ml
Purity
Protein A or G purified

Notes

200ug/ml of antibody purified from Bioreactor Concentrate by Protein A or G. Prepared in 10 mM PBS with 0.05% BSA & 0.05% azide. Also available WITHOUT BSA & azide at 1.0 mg/ml. (NBP2-47879)

Antibody with azide - store at 2 to 8C. Antibody without azide - store at -20 to -80C. Antibody is stable for 24 months. Non-hazardous.

Alternate Names for CD43/Sialophorin Antibody (SPN/839)

  • CD43 antigen
  • CD43
  • CD43)
  • Galactoglycoprotein
  • GALGP
  • GPL115
  • Leukocyte sialoglycoprotein
  • Leukosialin
  • LSN
  • Ly-48
  • sialophorin (gpL115, leukosialin, CD43)
  • Sialophorin
  • SPN

Background

CD43 is a key glycoprotein of thymocytes and T lymphocytes, which affects the physicochemical properties of the T-cell surface and plays a role in lectin binding. This antibody contains an extended rodlike structure that may protrude above the glycocalyx of the cell, allowing accessibility of multiple glycan chains for binding. During T-cell activation CD43 is actively removed from the T-cell-antigen-presenting cell contact site, thus suggesting CD43's role in negative regulation of the adaptive immune response.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Secondary Antibodies

 

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Bioinformatics

Gene Symbol SPN
Uniprot