CD43/Sialophorin Antibody (R2/60)

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Flow Cytometry: CD43/Sialophorin Antibody (R2/60) [NBP1-43413] - Analysis using the FITC conjugate of NBP1-43413. Staining of C57BL/6 splenocytes with Anti-Human/Mouse CD45R (B220) APC and 0.06 ug of Rat IgM kappa ...read more
Flow Cytometry: CD43/Sialophorin Antibody (R2/60) [NBP1-43413] - Staining of C57Bl/6 splenocytes with 0.25 ug of Rat IgM Isotype Control Purified (open histogram) or 0.25 ug of Anti-Mouse CD43 Purified (filled ...read more
Flow Cytometry: CD43/Sialophorin Antibody (R2/60) [NBP1-43413] - Analysis using the Biotin conjugate of NBP1-43413. Staining of C57BL/6 splenocytes with Anti-Human/Mouse CD45R (B220) APC and 0.25 ug of Rat IgM kappa ...read more

Product Details

Summary
Reactivity MuSpecies Glossary
Applications WB, Flow, IP
Clone
R2/60
Clonality
Monoclonal
Host
Rat
Conjugate
Unconjugated
Concentration
0.5 mg/ml

CD43/Sialophorin Antibody (R2/60) Summary

Immunogen
The immunogen for this antibody was CD43.
Isotype
IgM
Clonality
Monoclonal
Host
Rat
Gene
SPN
Purity
Protein A or G purified
Innovator's Reward
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Applications/Dilutions

Dilutions
  • Western Blot 1:100-1:2000
  • Flow Cytometry 1:10-1:1000
  • Immunoprecipitation 1:10-1:500
Application Notes
This R2/60 antibody has been tested by flow cytometric analysis of mouse splenocytes. This can be used at less than or equal to 0.5 ug per test. Cell number should be determined empirically but can range from 10^5to 10^8cells/test.

Reactivity Notes

This antibody is reactive to Mouse.

Packaging, Storage & Formulations

Storage
Store at 4C. Do not freeze.
Buffer
Aqueous, may contain carrier protein/stabilizer.
Preservative
0.09% Sodium Azide
Concentration
0.5 mg/ml
Purity
Protein A or G purified

Alternate Names for CD43/Sialophorin Antibody (R2/60)

  • CD43 antigen
  • CD43
  • CD43)
  • Galactoglycoprotein
  • GALGP
  • GPL115
  • Leukocyte sialoglycoprotein
  • Leukosialin
  • LSN
  • Ly-48
  • sialophorin (gpL115, leukosialin, CD43)
  • Sialophorin
  • SPN

Background

The R2/60 monoclonal antibody reacts with mouse CD43, also known as leukosialin and sialophorin. CD43 has glycoforms of approximately 115 and 130 kDa, and is expressed by an early B cell subset in BM, thymocytes and mature T cells, NK cells, myeloid lineage cells and platelets. CD43 is reported to bind to CD54 and play a role in adhesion and B cell survival. While the S7 and 1B11 monoclonal antibody clones detect only certain isoforms of CD43, data suggests that R2/60 detects the expression of both isoforms. It has been demonstrated that R2/60 stains the majority of CD4+ and CD8+ splenocytes, as well as a significant population of CD19+ splenocytes. However, the clones S7 and 1B11 stain only CD4+ and CD8+, or CD19+ splenocytes, respectively. This suggests that S7 and 1B11 recognize distinct epitopes which are differentially expressed by splenic B and T cells, whereas R2/60 recognizes an epitope shared by both isoforms of CD43.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Product General Protocols

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Video Protocols

WB Video Protocol

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Secondary Antibodies

 

Isotype Controls

Other Available Formats

Alexa Fluor 405 Labeled NBP1-43413AF405
Alexa Fluor 488 Labeled NBP1-43413AF488
Alexa Fluor 647 Labeled NBP1-43413AF647
Alexa Fluor 700 Labeled NBP1-43413AF700
Allophycocyanin Labeled NBP1-43413APC
Biotin Labeled NBP1-43647
DyLight 350 Labeled NBP1-43413UV
DyLight 405 Labeled NBP1-43413V
DyLight 488 Labeled NBP1-43413G
DyLight 680 Labeled NBP1-43413FR
DyLight 755 Labeled NBP1-43413IR
FITC Labeled NBP1-44011
PE Labeled NBP1-43413PE
PerCP Labeled NBP1-43413PCP

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Bioinformatics

Gene Symbol SPN
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