CD43/Sialophorin Antibody (Bra7G) - Azide and BSA Free

Immunocytochemistry/ Immunofluorescence: CD43/Sialophorin Antibody (Bra7G) - Azide and BSA Free [NBP3-11562] - Immunofluorescence Analysis of K562 cells labeling CD43 with CD43/Sialophorin Antibody (Bra7G) followed by Goat anti-Mouse IgG-CF488 (Green). The nuclear counterstain is NucSpot .

Immunohistochemistry-Paraffin: CD43/Sialophorin Antibody (Bra7G) - Azide and BSA Free [NBP3-11562] - Human Spleen stained with CD43 Monoclonal Antibody (Bra7G).

• Reactivity: Hu
• Applications: Flow, ICC/IF, IHC
• Clone: Bra7G
• Clonality: Monoclonal
• Host: Mouse
• Conjugate: Unconjugated
• Format: Azide and BSA Free
• Concentration: 1 mg/ml

CD43/Sialophorin Antibody (Bra7G) - Azide and BSA Free Summary

• Description: 1.0 mg/ml of antibody purified from Bioreactor Concentrate by PEG precipitation. Prepared in 10mM PBS WITHOUT BSA & azide. Also available at 200 ug/ml WITH BSA & azide (NBP2-44650).
  
  Antibody with azide - store at 2 to 8C. Antibody without azide - store at -20 to -80C.
• Additional Information: Clone Bra7G was used by HLDA to establish CD designation.
• Immunogen: Immature pluripotent human leukemia K562 cells
• Localization: Cell surface
• Marker: T-Cell Marker
• Specificity: It recognizes a cell surface glycoprotein of 95/115/135kDa (depending upon the extent of glycosylation), identified as CD43 (Workshop V). Epitope of monoclonal antibody Bra7G is clearly different from that of monoclonal antibody DF-T1, called b as opposed to a for DF-T1. 70-90% of T-cell lymphomas and from 22-37% of B-cell lymphomas express CD43. No reactivity has been observed with reactive B-cells. So a B-lineage population that co-expresses CD43 is highly likely to be a malignant lymphoma, especially a low-grade lymphoma, rather than a reactive B-cell population. When CD43 antibody is used in combination with anti-CD20, effective immunophenotyping of the lymphomas in formalin-fixed tissues can be obtained. Co-staining of a lymphoid infiltrate with anti-CD20 and anti-CD43 argues against a reactive process and favors a diagnosis of lymphoma.
• Isotype: IgM Kappa
• Clonality: Monoclonal
• Host: Mouse
• Gene: SPN
• Purity: PEG purified

Applications/Dilutions

• Dilutions:
  • Flow Cytometry 0.5 - 1 ug/million cells in 0.1 ml
  • Immunocytochemistry/ Immunofluorescence
  • Immunohistochemistry
  • Immunohistochemistry-Paraffin 0.5 - 1.0 ug/ml
• Application Notes: Immunohistochemistry (Formalin-fixed): 1-2ug/ml for 30 minutes at RT. For staining of formalin-fixed tissues, digest sections with trypsin at 1mg/ml PBS, 15 min at RT.
  Optimal dilution for a specific application should be determined.

Packaging, Storage & Formulations

• Storage: Store at -20 to -80C. Avoid freeze-thaw cycles.
• Buffer: 10 mM PBS
• Preservative: No Preservative
• Concentration: 1 mg/ml
• Purity: PEG purified

Alternate Names for CD43/Sialophorin Antibody (Bra7G) - Azide and BSA Free

• CD43 antigen
• CD43
• CD43)
• Galactoglycoprotein
• GALGP
• GPL115
• Leukocyte sialoglycoprotein
• Leukosialin
• LSN
• Ly-48
• sialophorin (gpL115, leukosialin, CD43)
• Sialophorin
• SPN

Background

CD43 is a key glycoprotein of thymocytes and T lymphocytes, which affects the physicochemical properties of the T-cell surface and plays a role in lectin binding. This antibody contains an extended rodlike structure that may protrude above the glycocalyx of the cell, allowing accessibility of multiple glycan chains for binding. During T-cell activation CD43 is actively removed from the T-cell-antigen-presenting cell contact site, thus suggesting CD43's role in negative regulation of the adaptive immune response.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Bioinformatics

• Gene Symbol: SPN