Reactivity | HuSpecies Glossary |
Applications | WB, Flow, CyTOF-ready |
Clonality | Polyclonal |
Host | Sheep |
Conjugate | Unconjugated |
Concentration | LYOPH |
Immunogen | Mouse myeloma cell line NS0-derived recombinant human CD42b/GPIb alpha His17-Leu505 Accession # P07359 |
Specificity | Detects human CD42b/GPIb alpha in direct ELISAs and Western blots. |
Source | N/A |
Isotype | IgG |
Clonality | Polyclonal |
Host | Sheep |
Gene | GP1BA |
Purity Statement | Antigen Affinity-purified |
Innovator's Reward | Test in a species/application not listed above to receive a full credit towards a future purchase. |
Dilutions |
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Publications |
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Storage | Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
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Buffer | Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS. |
Preservative | No Preservative |
Concentration | LYOPH |
Reconstitution Instructions | Reconstitute at 0.2 mg/mL in sterile PBS. |
Platelet glycoprotein Ib alpha chain (GPIb alpha ), also known as CD42b alpha , is a 145 kDa type I transmembrane protein that is a member of the leucine-rich repeat (LRR) family of ligand binding proteins (1‑3). It is expressed by platelets as the ligand-binding subunit of the platelet GPIb-IX-V complex (4). Human GPIb alpha contains a 16 amino acid (aa) signal sequence, a 489 aa extracellular domain (ECD), a 21-aa transmembrane domain, and a 100 aa cytoplasmic region. The ECD contains 8 LRRs, with # 2, 3, and 4 having been demonstrated to regulate shear-dependent adhesion to von Willebrand factor (vWF) (5, 6). The LRRs are followed by a thrombin-binding anionic region that includes three sulfated tyrosines, a sialomucin domain with N- and O-linked carbohydrates, and two cysteines near the membrane that allow dimerization with GP1b alpha beta (1‑6). Four human isoforms with 1 to 4 repeats of aa 398‑411 within the sialomucin domain of mature GPIb alpha are known to exist but have unknown significance (7). The ECD of human GPIb alpha shares 48‑51% aa identity with mouse, rat, bovine, and canine GPIb alpha . The metalloproteinase TACE/ADAM17 constitutively and inducibly cleaves GPIb alpha , between Gly480 and Val481. This releases a soluble form called glycocalicin that circulates at ~2 μg/mL (8, 9). GPIb alpha binding to ligands such as thrombin, kininogen, and coagulation factors XI and XII helps to initiate platelet activation and coordinate the coagulation cascade (1, 10‑12). Binding of GPIb alpha to vWF or thrombospondin in the plasma or matrix, vWF or P-selectin on endothelial cells, or the integrin alpha M beta 2 (MAC-1) on myeloid cells, controls response to vascular injury (1, 13). Bernard-Soulier syndrome and platelet-type von Willebrand disease are platelet function disorders that can be caused by mutations in GPIb alpha (1, 14).
Secondary Antibodies |
Isotype Controls |
Diseases for CD42b/GPIb alpha Antibody (AF4067)Discover more about diseases related to CD42b/GPIb alpha Antibody (AF4067).
| Pathways for CD42b/GPIb alpha Antibody (AF4067)View related products by pathway.
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PTMs for CD42b/GPIb alpha Antibody (AF4067)Learn more about PTMs related to CD42b/GPIb alpha Antibody (AF4067).
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