CD42b/GPIb alpha Antibody [Unconjugated]

Western blot shows lysates of human platelets. PVDF membrane was probed with 1 µg/mL of Sheep Anti-Human CD42b/GPIb alpha Antigen Affinity-purified Polyclonal Antibody (Catalog # AF4067) followed by HRP-conjugated Anti-Sheep IgG Secondary Antibody (Catalog # HAF016). A specific band was detected for CD42b/GPIb alpha at approximately 135 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.

Human whole blood CD41+ platelets were stained with Sheep Anti-Human CD42b/GPIb alpha Polyclonal Antibody (Catalog # AF4067) followed by NL637-conjugated anti sheep antibody (Catalog # NL011) and Human CD41 FITC-conjugated Monoclonal Antibody. Quadrant markers were set based on control antibody staining (Catalog # 5‑001‑A).

• Reactivity: Hu
• Applications: WB, Flow, CyTOF-ready
• Clonality: Polyclonal
• Host: Sheep
• Conjugate: Unconjugated
• Concentration: LYOPH

CD42b/GPIb alpha Antibody [Unconjugated] Summary

• Immunogen: Mouse myeloma cell line NS0-derived recombinant human CD42b/GPIb alpha
  His17-Leu505
  Accession # P07359
• Specificity: Detects human CD42b/GPIb alpha in direct ELISAs and Western blots.
• Source: N/A
• Isotype: IgG
• Clonality: Polyclonal
• Host: Sheep
• Gene: GP1BA
• Purity Statement: Antigen Affinity-purified

Applications/Dilutions

• Dilutions:
  • CyTOF-ready
  • Flow Cytometry 2.5 ug/10^6 cells
  • Western Blot 1 ug/mL

Packaging, Storage & Formulations

• Storage: Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.
• Buffer: Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
• Preservative: No Preservative
• Concentration: LYOPH
• Reconstitution Instructions: Reconstitute at 0.2 mg/mL in sterile PBS.

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for CD42b/GPIb alpha Antibody [Unconjugated]

• Antigen CD42b-alpha
• BP1BA
• BSS
• CD42b antigen
• CD42b
• CD42b-alpha
• glycoprotein Ib (platelet), alpha polypeptide
• Glycoprotein Ibalpha
• GP1B
• GP1BA
• GPIb alpha
• GP-Ib alpha
• GPIbA
• GPIb-alpha
• MGC34595
• platelet glycoprotein Ib alpha chain
• platelet membrane glycoprotein 1b-alpha subunit

Background

Platelet glycoprotein Ib alpha chain (GPIb  alpha ), also known as CD42b alpha , is a 145 kDa type I transmembrane protein that is a member of the leucine-rich repeat (LRR) family of ligand binding proteins (1‑3). It is expressed by platelets as the ligand-binding subunit of the platelet GPIb-IX-V complex (4). Human GPIb  alpha contains a 16 amino acid (aa) signal sequence, a 489 aa extracellular domain (ECD), a 21-aa transmembrane domain, and a 100 aa cytoplasmic region. The ECD contains 8 LRRs, with # 2, 3, and 4 having been demonstrated to regulate shear-dependent adhesion to von Willebrand factor (vWF) (5, 6). The LRRs are followed by a thrombin-binding anionic region that includes three sulfated tyrosines, a sialomucin domain with N- and O-linked carbohydrates, and two cysteines near the membrane that allow dimerization with GP1b  alpha beta (1‑6). Four human isoforms with 1 to 4 repeats of aa 398‑411 within the sialomucin domain of mature GPIb  alpha are known to exist but have unknown significance (7). The ECD of human GPIb  alpha shares 48‑51% aa identity with mouse, rat, bovine, and canine GPIb  alpha . The metalloproteinase TACE/ADAM17 constitutively and inducibly cleaves GPIb  alpha , between Gly480 and Val481. This releases a soluble form called glycocalicin that circulates at ~2 μg/mL (8, 9). GPIb  alpha binding to ligands such as thrombin, kininogen, and coagulation factors XI and XII helps to initiate platelet activation and coordinate the coagulation cascade (1, 10‑12). Binding of GPIb  alpha to vWF or thrombospondin in the plasma or matrix, vWF or P-selectin on endothelial cells, or the integrin  alpha M beta 2 (MAC-1) on myeloid cells, controls response to vascular injury (1, 13). Bernard-Soulier syndrome and platelet-type von Willebrand disease are platelet function disorders that can be caused by mutations in GPIb  alpha (1, 14).

1. Andrews, R.K. et al. (2007) Arterioscler. Thromb. Vasc. Biol. 27:1511. 
2. Lopez, J.A. et al. (1987) Proc. Natl. Acad. Sci. USA 84:5615.
3. Wenger, R.H. et al. (1988) Biochem. Biophys. Res. Commun. 156:389. 
4. Luo, S-Z. et al. (2007) Blood 109:603. 
5. Uff, S. et al. (2002) J. Biol. Chem. 277:35657.
6. Shen, Y. et al. (2006) J. Biol. Chem. 281:26419.
7. Ishida, F. et al. (1995) Blood 86:1356.
8. Gardiner, E.E. et al. (2007) J. Thromb. Haemost. 5:1530.
9. Beer, J.H. et al. (1994) Blood 83:691.
10. Adam, F. et al. (2003) Eur. J. Biochem. 270:2959.
11. Baglia, F.A. et al. (2004) J. Biol. Chem. 279:49323.
12. Bradford, H.N. et al. (2000) J. Biol. Chem. 275:22756.
13. Wang, Y. et al. (2005) Circulation 112:2993.
14. Othman, M. et al. (2005) Blood 105:4330.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Publications for CD42b/GPIb alpha Antibody (AF4067)(2)

Publications using AF4067

• LS Oliveira, MI Estevão-Co, VG Alvarenga, DE Vivas-Ruiz, A Yarleque, AM Lima, A Cavaco, JA Eble, EF Sanchez Atroxlysin-III, A Metalloproteinase from the Venom of the Peruvian Pit Viper Snake Bothrops atrox (Jerg�n) Induces Glycoprotein VI Shedding and Impairs Platelet Function Molecules, 2019;24(19):. 2019 [PMID: 31561469] (Western Blot, Human)
• Arlinghaus F, Eble J The collagen-binding integrin alpha2beta1 is a novel interaction partner of the Trimeresurus flavoviridis venom protein flavocetin-A. J Biol Chem, 2013;288(2):947-55. 2013 [PMID: 23204528] (Western Blot, Human)

Bioinformatics

• Gene Symbol: GP1BA
• Uniprot:
  • P07359()