CD42b/GPIb alpha Antibody (486805) [Alexa Fluor® 700]

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Product Details

Summary
Reactivity HuSpecies Glossary
Applications Flow
Clone
486805
Clonality
Monoclonal
Host
Mouse
Conjugate
Alexa Fluor 700
Concentration
Please see the vial label for concentration. If unlisted please contact technical services.

CD42b/GPIb alpha Antibody (486805) [Alexa Fluor® 700] Summary

Immunogen
Mouse myeloma cell line NS0-derived recombinant human CD42b/GPIb alpha
His17-Leu505
Accession # P07359
Specificity
Detects human CD42b/GPIb alpha  in direct ELISAs and Western blots.
Isotype
IgG1
Clonality
Monoclonal
Host
Mouse
Gene
GP1BA
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.

Applications/Dilutions

Dilutions
  • Flow Cytometry 0.25-1 ug/10^6 cells
Application Notes
Flow Cytometry: Please use 0.25-1 ug of conjugated antibody per 10e6 cells.

Packaging, Storage & Formulations

Storage
Store the unopened product at 2 - 8 °C. Do not use past expiration date.
Buffer
Supplied 0.2 mg/mL in a saline solution containing BSA and Sodium Azide.
Preservative
0.09% Sodium Azide
Concentration
Please see the vial label for concentration. If unlisted please contact technical services.

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for CD42b/GPIb alpha Antibody (486805) [Alexa Fluor® 700]

  • Antigen CD42b-alpha
  • BP1BA
  • BSS
  • CD42b antigen
  • CD42b
  • CD42b-alpha
  • glycoprotein Ib (platelet), alpha polypeptide
  • Glycoprotein Ibalpha
  • GP1B
  • GP1BA
  • GPIb alpha
  • GP-Ib alpha
  • GPIbA
  • GPIb-alpha
  • MGC34595
  • platelet glycoprotein Ib alpha chain
  • platelet membrane glycoprotein 1b-alpha subunit

Background

Platelet glycoprotein Ib alpha chain (GPIb  alpha ), also known as CD42b, is a 145 kDa type I transmembrane protein that is a member of the leucine-rich repeat (LRR) family of ligand binding proteins (1‑3). It is expressed by platelets as the ligand-binding subunit of the platelet GPIb-IX-V complex (4). Human CD42b contains a 16 amino acid (aa) signal sequence, a 489 aa extracellular domain (ECD), a 21-aa transmembrane domain, and a 100 aa cytoplasmic region. The ECD contains 8 LRRs, with # 2, 3, and 4 having been demonstrated to regulate shear-dependent adhesion to von Willebrand factor (vWF) (5, 6). The LRRs are followed by a thrombin-binding anionic region that includes three sulfated tyrosines, a sialomucin domain with N- and O-linked carbohydrates, and two cysteines near the membrane that allow dimerization (1‑6). Four human isoforms with 1 to 4 repeats of aa 398‑411 within the sialomucin domain of mature CD42b are known to exist but have unknown significance (7). The ECD of human CD42b shares 48‑51% aa identity with mouse, rat, bovine, and canine CD42b. The metalloproteinase TACE/ADAM17 constitutively and inducibly cleaves CD42b, between Gly480 and Val481. This releases a soluble form called glycocalicin that circulates at ~2 μg/mL (8, 9). CD42b binding to ligands such as thrombin, kininogen, and coagulation factors XI and XII helps to initiate platelet activation and coordinate the coagulation cascade (1, 10‑12). Binding of CD42b to vWF or thrombospondin in the plasma or matrix, vWF or P-selectin on endothelial cells, or the integrin  alpha M beta 2 (MAC-1) on myeloid cells, controls response to vascular injury (1, 13). Bernard-Soulier syndrome and platelet-type von Willebrand disease are platelet function disorders that can be caused by mutations in CD42b (1, 14).

  1. Andrews, R.K. et al. (2007) Arterioscler. Thromb. Vasc. Biol. 27:1511. 
  2. Lopez, J.A. et al. (1987) Proc. Natl. Acad. Sci. USA 84:5615.
  3. Wenger, R.H. et al. (1988) Biochem. Biophys. Res. Commun. 156:389. 
  4. Luo, S-Z. et al. (2007) Blood 109:603. 
  5. Uff, S. et al. (2002) J. Biol. Chem. 277:35657.
  6. Shen, Y. et al. (2006) J. Biol. Chem. 281:26419.
  7. Ishida, F. et al. (1995) Blood 86:1356.
  8. Gardiner, E.E. et al. (2007) J. Thromb. Haemost. 5:1530.
  9. Beer, J.H. et al. (1994) Blood 83:691.
  10. Adam, F. et al. (2003) Eur. J. Biochem. 270:2959.
  11. Baglia, F.A. et al. (2004) J. Biol. Chem. 279:49323.
  12. Bradford, H.N. et al. (2000) J. Biol. Chem. 275:22756.
  13. Wang, Y. et al. (2005) Circulation 112:2993.
  14. Othman, M. et al. (2005) Blood 105:4330.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Bioinformatics

Gene Symbol GP1BA
Entrez
Uniprot