CD42b/GPIb alpha Antibody (1C6) - Azide and BSA Free

Immunoprecipitation: CD42b/GPIb alpha Antibody (1C6) [H00002811-M02] - Analysis of GP1BA transfected lysate using anti-GP1BA monoclonal antibody and Protein A Magnetic Bead, and immunoblotted with GP1BA MaxPab rabbit polyclonal antibody.

ELISA: CD42b/GPIb alpha Antibody (1C6) [H00002811-M02] - Detection limit for recombinant GST tagged GP1BA is approximately 0.03ng/ml as a capture antibody.

• Reactivity: Hu
• Applications: WB, ELISA, IP
• Clone: 1C6
• Clonality: Monoclonal
• Host: Mouse
• Conjugate: Unconjugated
• Format: Azide and BSA Free

CD42b/GPIb alpha Antibody (1C6) - Azide and BSA Free Summary

• Description: Quality control test: Antibody Reactive Against Recombinant Protein.
• Immunogen: GP1BA (AAH27955, 19 a.a. ~ 128 a.a) partial recombinant protein with GST tag. MW of the GST tag alone is 26 KDa. ICEVSKVASHLEVNCDKRNLTALPPDLPKDTTILHLSENLLYTFSLATLMPYTRLTQLNLDRCELTKLQVDGTLPVLGTLDLSHNQLQSLPLLGQTLPALTVLDVPFNRL
• Specificity: glycoprotein Ib (platelet), alpha polypeptide
• Isotype: IgG2a Kappa
• Clonality: Monoclonal
• Host: Mouse
• Gene: GP1BA
• Purity: IgG purified

Applications/Dilutions

• Dilutions:
  • ELISA
  • Immunoprecipitation
  • Western Blot 1:500
• Application Notes: Antibody reactivity against Recombinant Protein with GST tag on ELISA and WB. GST tag alone is used as a negative control.

Packaging, Storage & Formulations

• Storage: Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles.
• Buffer: In 1x PBS, pH 7.4
• Preservative: No Preservative
• Purity: IgG purified

Notes

This product is produced by and distributed for Abnova, a company based in Taiwan.

Alternate Names for CD42b/GPIb alpha Antibody (1C6) - Azide and BSA Free

• Antigen CD42b-alpha
• BP1BA
• BSS
• CD42b antigen
• CD42b
• CD42b-alpha
• glycoprotein Ib (platelet), alpha polypeptide
• Glycoprotein Ibalpha
• GP1B
• GP1BA
• GPIb alpha
• GP-Ib alpha
• GPIbA
• GPIb-alpha
• MGC34595
• platelet glycoprotein Ib alpha chain
• platelet membrane glycoprotein 1b-alpha subunit

Background

Glycoprotein Ib (GP Ib) is a platelet surface membrane glycoprotein composed of a heterodimer, an alpha chain and a beta chain, that are linked by disulfide bonds. The Gp Ib functions as a receptor for von Willebrand factor (VWF). The complete receptor complex includes noncovalent association of the alpha and beta subunits with platelet glycoprotein IX and platelet glycoprotein V. The binding of the GP Ib-IX-V complex to VWF facilitates initial platelet adhesion to vascular subendothelium after vascular injury, and also initiates signaling events within the platelet that lead to enhanced platelet activation, thrombosis, and hemostasis. This gene encodes the alpha subunit. Several polymorphisms and mutations have been described in this gene, some of which are the cause of Bernard-Soulier syndromes and platelet-type von Willebrand disease.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Publications for CD42b/GPIb alpha Antibody (H00002811-M02)(1)

Publication using H00002811-M02

• Qian-Yun S, Cai-E W, Ya-Nan L et al. Inhibition of platelet aggregation and blood coagulation by a P-III class metalloproteinase purified from Naja atra venom. Toxicon. 2020-09-21 [PMID: 32971099]

Bioinformatics

• Gene Symbol: GP1BA
• Entrez:
  • 2811(Human)
• OMIM:
  • 177820(Human)
• Uniprot:
  • AAH27955(Human)