Human CD40/TNFRSF5 ELISA Kit (Colorimetric)

Human CD40/TNFRSF5 ELISA Kit [NBP1-91264] - Data for Human sCD40 Platinum ELISA

• Reactivity: Hu
• Applications: ELISA
• Suitable Sample Type: Cell culture supernatant, serum, plasma (EDTA, heparin), amniotic fluid
• Standard Curve Range: 7.8 - 500 pg/mL
• Sensitivity: 1.3 pg/mL

Human CD40/TNFRSF5 ELISA Kit (Colorimetric) Summary

• Description: This kit is an enzyme-linked immunosorbent assay for quantitative detection.
• Specificity: This antibody detects the soluble protein. The interference of circulating factors of the immune system was evaluated by spiking these proteins at physiologically relevant concentrations into a human CD40 positive serum. There was no crossreactivity detected.
• Standard Curve Range: 7.8 - 500 pg/mL
• Sensitivity: 1.3 pg/mL
• Assay Type: Sandwich ELISA
• Inter-Assay: CV% < 7.00%
• Intra-Assay: CV% < 5.50%
• Spike Recovery: The amount of endogenous human CD40 in unspiked serum was subtracted from the spike values. The recovery ranged from 81% to 110% with an overall mean recovery of 94%.
• Sample Volume: 50 uL
• Kit Type: ELISA Kit (Colorimetric)
• Gene: CD40

Applications/Dilutions

• Dilutions:
  • ELISA

Packaging, Storage & Formulations

• Storage: Storage of components varies. See protocol for specific instructions.

Alternate Names for Human CD40/TNFRSF5 ELISA Kit (Colorimetric)

• B cell surface antigen CD40
• B-cell surface antigen CD40
• Bp50B cell-associated molecule
• CD40 antigen
• CD40 molecule, TNF receptor superfamily member 5
• CD40 type II isoform
• CD40
• CD40L receptor
• CDw40
• MGC9013
• nerve growth factor receptor-related B-lymphocyte activation molecule
• p50
• TNFRSF5
• TNFRSF5CD40 antigen (TNF receptor superfamily member 5)
• tumor necrosis factor receptor superfamily member 5
• tumor necrosis factor receptor superfamily, member 5

Background

CD40 is a 48 kD type I glycoprotein and a member of the TNFR superfamily primarily expressed on B cells, macrophages, follicular dendritic cells, endothelial cells, fibroblasts, and at low levels on plasma cells. CD40 is also expressed on a wide range of tumor cells, including non-Hodgkin's and Hodgkin's lymphomas, myeloma and some carcinomas including nasopharynx, bladder, cervix, kidney and ovary.The binding of CD154 (CD40L) on TH cells to CD40 activates antigen presenting cells and induces a variety of downstream effects.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. ELISA Kits are guaranteed for 6 months from date of receipt.

FAQs for CD40/TNFRSF5 ELISA Kit (NBP1-91264). (Showing 1 - 2 of 2 FAQ).

1. I wanna know does CD40 antibody for IHC-P comes with positive control slide?
   • Our CD40 antibodies do not come with positive control slides however you can use any type of primary carcinoma cell as a positive control. I recommend breast carcinoma as this is what we have tested our CD40 antibodies on.
2. We are studying CD40L in our system. It has been known that both CD40 and CD11b are receptors for CD40L. We need CD40 antibody to neutralize CD40 binding to CD40L, then we may prove what is function for CD11b binding to CD40L. Do you have any CD40 Ab which has block function only between CD40 and CD40L, but not between CD11b and CD40L?
   • Unfortunately, none of our CD40 antibodies have been specifically tested for this application.

Bioinformatics

• Gene Symbol: CD40