CD40/TNFRSF5 Antibody (LOB7/6)

Immunohistochemistry-Frozen: CD40/TNFRSF5 Antibody (LOB7/6) [NBP1-39556] - Staining of a human tonsil cryosection with Mouse anti Human CD40 antibody, clone LOB7/6 followed by the Histar Detection system. Low power

Flow Cytometry: CD40/TNFRSF5 Antibody (LOB7/6) [NBP1-39556] - Analysis using the FITC conjugate of NBP1-39556. Staining of human peripheral blood lymphocytes with MOUSE ANTI HUMAN CD40:FITC.

Immunohistochemistry-Paraffin: CD40/TNFRSF5 Antibody (LOB7/6) [NBP1-39556] - Staining of human spleen. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody concentration 10 ug/ml.

Flow Cytometry: CD40/TNFRSF5 Antibody (LOB7/6) [NBP1-39556] - Staining of human peripheral blood lymphocytes with MOUSE ANTI HUMAN CD40: RPE.

Product Discontinued

CD40/TNFRSF5 Antibody (LOB7/6) Summary

• Immunogen: Recognizes human CD40 cell surface antigen, a 48kD glycoprotein expressed by B lymphocytes and weakly by some monocytes. CD40 is involved in the process of B cell selection in germinal centers and is vital in T cell-B cell interactions.
• Specificity: Recognizes human CD40 cell surface antigen, a 48kD glycoprotein expressed by B lymphocytes and weakly by some monocytes. CD40 is involved in the process of B cell selection in germinal centers and is vital in T cell-B cell interactions.
• Isotype: IgG2a
• Clonality: Monoclonal
• Host: Mouse
• Gene: CD40
• Purity: Protein A purified

Applications/Dilutions

• Dilutions:
  • Flow Cytometry 20 ug/ml
  • Immunohistochemistry 1:10-1:500
  • Immunohistochemistry-Frozen 1ug/ml - 10ug/ml
  • Immunohistochemistry-Paraffin 10 ug/ml
  • Immunoprecipitation 1:10-1:500
• Application Notes: This antibody is useful in Immunoprecipitation, Flow Cytometry / , Immunohistochemistry-Paraffin (10 ug/ml), Immunohistochemistry-Frozen (1 - 10 ug/ml).

Reactivity Notes

Reacts with: Canine

Packaging, Storage & Formulations

• Storage: Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
• Buffer: TBS
• Preservative: 0.09% Sodium Azide
• Purity: Protein A purified

Alternate Names for CD40/TNFRSF5 Antibody (LOB7/6)

• B cell surface antigen CD40
• B-cell surface antigen CD40
• Bp50B cell-associated molecule
• CD40 antigen
• CD40 molecule, TNF receptor superfamily member 5
• CD40 type II isoform
• CD40
• CD40L receptor
• CDw40
• MGC9013
• nerve growth factor receptor-related B-lymphocyte activation molecule
• p50
• TNFRSF5
• TNFRSF5CD40 antigen (TNF receptor superfamily member 5)
• tumor necrosis factor receptor superfamily member 5
• tumor necrosis factor receptor superfamily, member 5

Background

CD40 is a costimulatory molecule of the TNF receptor superfamily and is expressed on many cell types, such as B cells, monocytes/macrophages, dendritic cells, endothelial cells, fibroblasts or vascular smooth muscle cells. Interaction of CD40 and its ligand CD154 (CD40L) is required for the generation of antibody responses to T-dependent antigens as well as for the development of germinal centers and memory B cells. In monocytes/macrophages CD40 engagement induces production of pro-inflammatory cytokines and chemokines. CD40-CD154 interactions are also critical for development of CD4 T cell-dependent effector functions. CD40 links innate and adaptive immune responses to bacterial stimuli and serves as an important regulator affecting functions of other costimulatory molecules.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

FAQs for CD40/TNFRSF5 Antibody (NBP1-39556). (Showing 1 - 3 of 3 FAQ).

1. I wanna know does CD40 antibody for IHC-P comes with positive control slide?
   • Our CD40 antibodies do not come with positive control slides however you can use any type of primary carcinoma cell as a positive control. I recommend breast carcinoma as this is what we have tested our CD40 antibodies on.
2. We are studying CD40L in our system. It has been known that both CD40 and CD11b are receptors for CD40L. We need CD40 antibody to neutralize CD40 binding to CD40L, then we may prove what is function for CD11b binding to CD40L. Do you have any CD40 Ab which has block function only between CD40 and CD40L, but not between CD11b and CD40L?
   • Unfortunately, none of our CD40 antibodies have been specifically tested for this application.
3. Hello. I would like to know, for 0.05mg CD40 antibody (LOB7/6), how many slides i can produce?
   • Unfortunately there is not a way for us to estimate the number of slides you can produce using our product. This will depend on many factors. Depending on your sample type and how highly expressed the protein is you may need to optimize the concentration. It will depend on how much you load and in what volume of solution. The size of your slides, etc.

Bioinformatics

• Gene Symbol: CD40