CD40/TNFRSF5 Antibody (82111) [Phycoerythrin]

• Applications: Flow
• Clone: 82111
• Clonality: Monoclonal
• Host: Mouse
• Conjugate: Phycoerythrin

CD40/TNFRSF5 Antibody (82111) [Phycoerythrin] Summary

• Immunogen: Mouse myeloma cell line NS0-derived recombinant human CD40/TNFRSF5
  Glu21-Arg193
  Accession # P25942
• Specificity: Detects human CD40/TNFRSF5 in direct ELISAs and Western blots. In direct ELISAs, does not cross-react with recombinant human (rh) 4‑1BB, rhCD27, rhCD30, rhDR3, rhDR6, rhEDAR, rhFas, rhGITR, rhHVEM, rhLTR beta , rhNGF R, rhOPG, rhRANK, rhTAJ, rhTNF RI, rhTNF RII, or recombinant mouse CD40.
• Source: N/A
• Isotype: IgG2b
• Clonality: Monoclonal
• Host: Mouse
• Gene: CD40
• Purity Statement: Protein A or G purified from hybridoma culture supernatant

Packaging, Storage & Formulations

• Storage: Store the unopened product at 2 - 8° C. Do not use past expiration date. Protect from light.
• Buffer: Supplied in a saline solution containing BSA and Sodium Azide.
• Preservative: Sodium Azide

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Background

CD40, also known as TNFRSF5, is a 45-50 kD type I transmembrane glycoprotein that belongs to the TNF receptor superfamily. Mature human CD40 is 257 amino acids (aa) in length, and consists of a 173 aa extracellular domain (ECD), a 22 aa transmembrane segment and a 62 aa cytoplasmic region. The ECD contains four cysteine-rich repeats, the second of which is considered crucial to ligand binding. Over aa 21-193, human CD40 shares 58% aa sequence identity with mouse CD40. Although not ubiquitously expressed, cells known to constitutively or inducibly express CD40 are large in number and include basal layer keratinocytes, adipocytes, CD8⁺ T cells, fibrocytes (circulating stem cells), monocytes, eosinophils, dendritic cells, pancreatic duct epithelium, astrocytes, smooth muscle cells, fibroblasts, vascular endothelial cells and B cells. There is at least one alternative splice form that shows a 38 aa substitution for aa 166-277, generating a soluble molecule. There are two "ligands" for CD40, the first being CD154/CD40L, and the second Borrelia, a spirochete that causes Lyme disease. CD40 ligation by CD154 has many outcomes, some of which are cell-specific. Ligation of CD40 on B cells (with IL-4) induces IgE production, while bone marrow stromal cell activation via CD40 promotes OPG secretion with concomitant bone formation. In addition, stimulation of CD40 on keratinocytes results in IL-8 and RANTES expression with subsequent leukocyte infiltration, while adipocyte activation via CD40 promotes local M1 polarization.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

FAQs for CD40/TNFRSF5 Antibody (FAB6321P). (Showing 1 - 2 of 2 FAQ).

1. I wanna know does CD40 antibody for IHC-P comes with positive control slide?
   • Our CD40 antibodies do not come with positive control slides however you can use any type of primary carcinoma cell as a positive control. I recommend breast carcinoma as this is what we have tested our CD40 antibodies on.
2. We are studying CD40L in our system. It has been known that both CD40 and CD11b are receptors for CD40L. We need CD40 antibody to neutralize CD40 binding to CD40L, then we may prove what is function for CD11b binding to CD40L. Do you have any CD40 Ab which has block function only between CD40 and CD40L, but not between CD11b and CD40L?
   • Unfortunately, none of our CD40 antibodies have been specifically tested for this application.

Bioinformatics

• Gene Symbol: CD40