CD36 Antibody (SM0)

Product Discontinued

CD36 Antibody (SM0) Summary

• Immunogen: Human tonsil cells and peripheral blood mononuclear cells.
• Localization: Integral membrane protein
• Marker: Endothelial Cell Marker
• Specificity: NB100-65522 recognizes the CD36 antigen, an 88kD cell surface glycoprotein expressed by platelets, monocytes/macrophages and also by some microvascular endothelium and erythroid precursors. CD36 is the receptor for thrombospondin. When expressed by monocytes and macrophages, CD36 acts as a receptor for oxidized LDL and for apoptotic polymorphonuclear cells. NB100-65522 partially inhibits collagen induced platelet aggregation and macrophage ingestion of apoptotic human neutrophils. It has been reported that this antibody may activate platelets as measured by fibrinogen binding, P-Selectin expression and Annexin V binding - for further details please contact our Technical Services team. This clone is also reported to block interaction of oxidised low-density lipoprotein (OXLDL) with CD36.
• Isotype: IgM
• Clonality: Monoclonal
• Host: Mouse
• Gene: CD36
• Purity: Ammonium sulfate precipitation

Applications/Dilutions

• Dilutions:
  • Flow Cytometry 1:10-1:20
  • Immunohistochemistry 1:10-1:500
  • Immunohistochemistry-Frozen 1:10-1:500
• Application Notes: For Flow Cytometry: Use 10 ul of the suggested working dilution to label 10^6 cells or 100 ul whole blood.
• Control:

  CD36 Overexpression Lysate

Packaging, Storage & Formulations

• Storage: Store at 4C. Do not freeze.
• Buffer: Phosphate Buffered Saline
• Preservative: 0.09% Sodium Azide
• Concentration: 1.0 mg/ml
• Purity: Ammonium sulfate precipitation

Alternate Names for CD36 Antibody (SM0)

• BDPLT10
• CD36 antigen (collagen type I receptor, thrombospondin receptor)
• CD36 molecule (thrombospondin receptor)
• CHDS7
• cluster determinant 36
• FAT
• Fatty acid translocase
• Glycoprotein IIIb
• GP3B
• GP4
• GPIIIB
• GPIV
• leukocyte differentiation antigen CD36
• PAS IV
• PAS-4 protein
• PASIV
• platelet glycoprotein 4
• platelet glycoprotein IV
• SCARB3
• scavenger receptor class B, member 3

Background

Originally discovered in platelets, cluster of differentiation 36, CD36, (also known as thrombospondin receptor, fatty acid translocase (FAT), platelet membrane glycoprotein IV (GPIV), and scavenger receptor class B, member 3 (SR-B3)) is a plasma membrane glycoprotein belonging to the class B scavenger receptor family (1,2). Human, mouse, and rat CD36 is synthesized as a 472 amino acid (a.a.) protein with a theoretical molecular weight of 53 kDa for the canonical isoform (3). Its domains include a short cytoplasmic tail at the N-terminal and C-terminal and a large extracellular loop flanked on each side by a transmembrane domain. The extracellular domain facilitates the update of fatty acids (FFAs), phospholipids, and cholesterol by forming two hydrophobic cavities, which was first modeled in the CD36 homologue, LIMP-2/ SCARB2 (4).

The expression of CD36 has been reported in platelets, erythrocytes, monocytes, differentiated adipocytes, skeletal muscle, mammary epithelial cells, spleen cells, some skin microdermal endothelial cells, and in cancer. Circulating levels of soluble CD36 (sCD36) has also been reported in chronic inflammatory disease such as type 2 diabetes and chronic kidney disease. CD36 participates in angiogenesis, innate immunity, and the clearance of apoptotic phagocytes. In lipid metabolism, CD36 functions as a macrophage receptor for oxidized LDL and as an adipocyte receptor/transporter for long-chain FFAs. Plasmodium falciparum, the parasite that causes malaria, binds CD36 via PfEMP1 proteins, tethering parasite-infected erythrocytes to endothelial receptors (5). Anti-CD36 isoantibodies have been detected in Type 1 CD36-deficient mothers and is implicated as the cause of fetal/neonatal alloimmune thrombocytopenia (6).

References

1) Febbraio, M., Hajjar, D. P., & Silverstein, R. L. (2001). CD36: a class B scavenger receptor involved in angiogenesis, atherosclerosis, inflammation, and lipid metabolism. The Journal of clinical investigation, 108(6), 785-791. PMID: 11560944

2) Silverstein RL, Febbraio M. (2000) CD36 and atherosclerosis. Curr Opin Lipidol. 2000 11(5):483-91. PMID: 11048891.

3) Endemann G, Stanton LW, Madden KS, Bryant CM, White RT, Protter AA. (1993) CD36 is a receptor for oxidized low density lipoprotein. J Biol Chem. 268(16):11811-6. PMID: 7685021.

4) Wang, J., & Li, Y. (2019). CD36 tango in cancer: signaling pathways and functions. Theranostics, 9(17), 4893-4908. PMID: 31410189

5) Hsieh FL, Turner L, Bolla JR, Robinson CV, Lavstsen T, Higgins MK. (2016) The structural basis for CD36 binding by the malaria parasite. Nat Commun. 7:12837. PMID: 27667267

6) Gruarin P, Ulliers D, Thorne RF, Alessio M. (2000) Methionine 156 in the immunodominant domain of CD36 contributes to define the epitope recognized by the NL07 MoAb. Mol Cell Biochem 214, 115-121. PMID: 11195795.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

FAQs for CD36 Antibody (NB100-65522). (Showing 1 - 1 of 1 FAQ).

1. What is the difference between NBP1-28455 and NB100-65522? What means SM 0 and SM Phi?
   • NBP1-28455 and NB100-65522 are both IgM antibodies for detecting CD36 in human samples. The Major difference is of immunogens employed for raising these antibodies. For NBP1-28455, the immunogen used was a peptide made to the amino acid region CD36, whereas for NB100-65522, human tonsil cells/peripheral blood mononuclear cells were used as immunogen. Moreover, these antibodies are from different clones and have been purified by different methods. SM 0 and SM Phi are the clone names.

Bioinformatics

• Gene Symbol: CD36
• Entrez:
  • 948(Human)
• Uniprot:
  • P16671()