CD34 Antibody (EP373Y)

Product Discontinued

CD34 Antibody (EP373Y) Summary

• Immunogen: ynthetic peptide (the amino acid sequence is considered to be commercially sensitive) within Human CD34 aa 350 to the C-terminus (C terminal). The exact sequence is proprietary.
• Marker: Hematopoietic Stem Cell Marker
• Isotype: IgG
• Clonality: Monoclonal
• Host: Rabbit
• Gene: CD34
• Purity: Protein A purified

Applications/Dilutions

• Dilutions:
  • Flow Cytometry 1:60
  • Immunocytochemistry/ Immunofluorescence 1: 100-250
  • Immunohistochemistry 1:10-1:500
  • Immunohistochemistry-Paraffin 1:500-1000
  • Immunoprecipitation 1:40
  • Western Blot 1:10000-100000
• Application Notes: Although theoretical MW is 41 kDa, due to heavy glycosylation, in Western blot this antibody detects a band at approximately 120 kDa.

Reactivity Notes

Reacts with: Loxodonta Africana (African bush elephant).

Packaging, Storage & Formulations

• Storage: Store at -20C. Avoid freeze-thaw cycles.
• Buffer: 59% PBS (pH 7.2), 0.05% BSA and 40% Glycerol
• Preservative: 0.01% Sodium Azide
• Purity: Protein A purified

Notes

Produced using Abcam's RabMab® technology. RabMab® technology is covered by the following U.S. Patents, No. 5,675,063 and/or 7,429,487.

Alternate Names for CD34 Antibody (EP373Y)

• CD34 antigenhematopoietic progenitor cell antigen CD34
• CD34 molecule
• CD34
• HPCA1

Background

CD34, also known as Cluster of Differentiation 34, is a transmembrane phosphoglycoprotein that is primarily known for being a surface marker for hematopoietic stem cells (HSCs) and progenitor cells (1,2). The CD34 protein consists of a heavily glycosylated extracellular domain, a single-pass transmembrane helix, and a cytoplasmic tail with PDZ binding motifs (1,3). Human CD34 has two protein isoforms consisting of 385 amino acids (aa) for the canonical isoform and 328 aa for isoform 2 (3,4). The canonical CD34 isoform has calculated theoretical molecular weight of 40 kDa and an observed molecular weight of ~115 kDa (1,3,4). L-Selectin (CD62L) is the most common ligand for CD34, but it has also been shown to bind CrkL (1,3).

CD34 has commonly been used as a marker for the diagnosis and classification of various diseases and pathologies including leukemia and solitary fibrous tumor (SFT) (2,5). In terms of immunohistochemistry and histopathology, CD34 has been the most common marker for SFT and is expressed in ~79% of cases (5). In addition to its use as a cell marker, CD34-postive (CD34+) hematopoietic stem cells have been used therapeutically in patients following radiation or chemotherapy due to their regenerative potential (6). There are several clinical trials showing promising results for CD34+ cell therapy for cardiovascular diseases including heart failure, ischemia, dilated cardiomyopathy, acute myocardial infarction, and angina (6). Besides hematopoietic lineages, CD34 is also expressed in non-hematopoietic cells including mesenchymal stem cells, endothelial cells and progenitors, fibrocytes, muscle satellite cells, and some cancer stem cells (1,3). While the clinical and cell therapy applications of CD34 as a cell marker is well documented, the function of CD34 is less understood but has been implicated in many cellular processes such as adhesion, proliferation, signal transduction, differentiation, and progenitor phenotype maintenance (1,3).

References

1. Sidney, L. E., Branch, M. J., Dunphy, S. E., Dua, H. S., & Hopkinson, A. (2014). Concise review: evidence for CD34 as a common marker for diverse progenitors. Stem cells (Dayton, Ohio), 32(6), 1380-1389. https://doi.org/10.1002/stem.1661

2. Krause, D. S., Fackler, M. J., Civin, C. I., & May, W. S. (1996). CD34: structure, biology, and clinical utility. Blood, 87(1), 1-13

3. Kapoor, S., Shenoy, S. P., & Bose, B. (2020). CD34 cells in somatic, regenerative and cancer stem cells: Developmental biology, cell therapy, and omics big data perspective. Journal of cellular biochemistry, 121(5-6), 3058-3069. https://doi.org/10.1002/jcb.29571

4. Uniprot (P28906)

5. Davanzo, B., Emerson, R. E., Lisy, M., Koniaris, L. G., & Kays, J. K. (2018). Solitary fibrous tumor. Translational gastroenterology and hepatology, 3, 94. https://doi.org/10.21037/tgh.2018.11.02

6. Prasad, M., Corban, M. T., Henry, T. D., Dietz, A. B., Lerman, L. O., & Lerman, A. (2020). Promise of autologous CD34+ stem/progenitor cell therapy for treatment of cardiovascular disease. Cardiovascular research, 116(8), 1424-1433. https://doi.org/10.1093/cvr/cvaa027

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Publications for CD34 Antibody (NBP1-44407)(4)

Publications using NBP1-44407

• Chen X. Argyrin F therapy attenuates carcinogensis of pancreatic adenocarcinoma (PDAC). Thesis. (IHC-P, Human)
• Hassanzadeh H, Matin MM, Naderi-Meshkin H et al. Using paracrine effects of Ad-MSCs on keratinocyte cultivation and fabrication of epidermal sheets for improving clinical applications Cell Tissue Bank 2018-08-13 [PMID: 30105667] (FLOW, Human)
• Hasmim Meriem, Bruno Stefania, Azzi Sandy et al. Isolation and characterization of renal cancer stem cells from patient-derived xenografts. Oncotarget 2016-01-01 [PMID: 26551931] (ICC, Mouse)
• Mathivanan I, Trepp C, Brunold C et al. Retinal differentiation of human bone marrow-derived stem cells by co-culture with retinal pigment epithelium in vitro Exp. Cell Res. 2015-04-10 [PMID: 25724900] (ICC/IF, Human)

FAQs for CD34 Antibody (NBP1-44407). (Showing 1 - 2 of 2 FAQs).

1. I wonder if you have a CD105 or CD34 antibody suitable for IHC that is specific for human and do not bind mouse?
   • We do not have any anti-human CD34 or CD105 antibodies that are confirmed to NOT detect the mouse protein. When we have tested an antibody and confirmed that it will not react with mouse samples, we will add Mu(-) to the datasheet, and unfortunately all of our CD105 and CD34 antibodies will either detect the mouse protein, or they have not been used in mouse samples before.
2. For CD34 antibody (NBP1-44407), under the box for Species Reactivity, it reads "Human. Mouse cross reactivity tested by western blot and Immunohistochemistry". Does this mean that this antibody is also good for Mouse samples?
   • This means the antibody will detect mouse CD34 in both Western blot and Immunohistochemistry specifically. At this time, it has not been tested for mouse reactivity in other applications.

Bioinformatics

• Gene Symbol: CD34
• Entrez:
  • 947(Human)
• Uniprot:
  • P28906()