CD34 Antibody (1H6) [Unconjugated]

CLL-1390 canine leukocytic round cell neoplasia cell line was stained with Mouse Anti-Canine CD34 Monoclonal Antibody (Catalog # MAB3346, filled histogram) or isotype control antibody (Catalog # MAB002, open histogram), followed by Allophycocyanin-conjugated Anti-Mouse IgG Secondary Antibody (Catalog # F0101B). View our protocol for Staining Membrane-associated Proteins.

• Reactivity: Ca
• Applications: WB, Flow, CyTOF-ready
• Clone: 1H6
• Clonality: Monoclonal
• Host: Mouse
• Conjugate: Unconjugated
• Concentration: LYOPH

CD34 Antibody (1H6) [Unconjugated] Summary

• Immunogen: COS-7 African green monkey SV40 transformed kidney fibroblast-like cell line-derived recombinant canine CD34
• Specificity: Detects canine CD34 in Western blots.
• Source: N/A
• Isotype: IgG1
• Clonality: Monoclonal
• Host: Mouse
• Gene: CD34
• Purity Statement: Protein A or G purified from hybridoma culture supernatant

Applications/Dilutions

• Dilutions:
  • CyTOF-ready
  • Flow Cytometry 0.25 ug/10^6 cells
  • Western Blot

Packaging, Storage & Formulations

• Storage: Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.
• Buffer: Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
• Preservative: No Preservative
• Concentration: LYOPH
• Reconstitution Instructions: Reconstitute at 0.5 mg/mL in sterile PBS.

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for CD34 Antibody (1H6) [Unconjugated]

• CD34 antigenhematopoietic progenitor cell antigen CD34
• CD34 molecule
• CD34
• HPCA1

Background

CD34 is a sialomucin molecule that is expressed on primitive hematopoietic stem cells and downregulated as they differentiate into mature cells. Although its precise function remains unknown, the pattern of expression of CD34 suggests that it plays a significant role in early hematopoiesis.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Publications for CD34 Antibody (MAB3346)(3)

Publications using MAB3346

• T Teshima, A Matsuoka, M Shiba, K Dairaku, H Matsumoto, R Suzuki, H Koyama Comparison of Properties of Stem Cells Isolated from Adipose Tissue and Lipomas in Dogs Stem Cells Int, 2019-11-20;2019(0):1609876. 2019-11-20 [PMID: 31827523] (Flow Cytometry, Canine)
• Feng Wang Acellular Endocardium as a Novel Biomaterial for the Intima of Tissue-Engineered Small-Caliber Vascular Grafts Artif Organs, 2016-12-01;40(12):E253-E265. 2016-12-01 [PMID: 27911030] (Functional Assay, Canine)
• McSweeney PA, Rouleau KA, Wallace PM, Bruno B, Andrews RG, Krizanac-Bengez L, Sandmaier BM, Storb R, Wayner E, Nash RA Characterization of monoclonal antibodies that recognize canine CD34. Blood, 1998-03-15;91(6):1977-86. 1998-03-15 [PMID: 9490680] (Flow Cytometry, Surface Plasmon Resonance, Western Blot, Canine)

FAQs for CD34 Antibody (MAB3346). (Showing 1 - 1 of 1 FAQs).

1. I wonder if you have a CD105 or CD34 antibody suitable for IHC that is specific for human and do not bind mouse?
   • We do not have any anti-human CD34 or CD105 antibodies that are confirmed to NOT detect the mouse protein. When we have tested an antibody and confirmed that it will not react with mouse samples, we will add Mu(-) to the datasheet, and unfortunately all of our CD105 and CD34 antibodies will either detect the mouse protein, or they have not been used in mouse samples before.

Bioinformatics

• Gene Symbol: CD34