Recombinant Human CD3 zeta His Protein

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SDS-Page: Recombinant Human CD3 Protein [NBP2-61462] - 15% SDS-Page (3ug)

Product Details

Summary
Reactivity HuSpecies Glossary
Applications PAGE
Concentration
0.2 mg/ml

Order Details

Recombinant Human CD3 zeta His Protein Summary

Description
A recombinant protein with a C-terminal His-tag and corresponding to the amino acids 52-164 of Human CD3.

Source: Baculovirus

Amino Acid Sequence: ADPRVKFSRS ADAPAYQQGQ NQLYNELNLG RREEYDVLDK RRGRDPEMGG KPQRRKNPQE GLYNELQKDK MAEAYSEIGM KGERRRGKGH DGLYQGLSTA TKDTYDALHM QALPPRHHHH HH

Source
Baculovirus
Protein/Peptide Type
Recombinant Protein
Gene
CD247
Purity
>90%, by SDS-PAGE
Endotoxin Note
< 1.0 EU per 1ug of protein (determined by LAL method)

Applications/Dilutions

Dilutions
  • SDS-Page
Theoretical MW
14.1 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Packaging, Storage & Formulations

Storage
Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
Buffer
20 mM Tris-HCl buffer (pH 6.8), 50% glycerol, 1 mM DTT, 1 mM EDTA, 0.1 M NaCl
Preservative
No Preservative
Concentration
0.2 mg/ml
Purity
>90%, by SDS-PAGE

Alternate Names for Recombinant Human CD3 zeta His Protein

  • CD247 antigen
  • CD247 antigen, zeta subunit
  • CD247 molecule
  • CD247
  • CD3 zeta
  • CD3H
  • CD3Q
  • CD3z antigen, zeta polypeptide (TiT3 complex)
  • CD3Z
  • CD3ZT-cell receptor T3 zeta chain
  • IMD25
  • T3Z
  • T-cell antigen receptor complex, zeta subunit of CD3
  • T-cell surface glycoprotein CD3 zeta chain
  • TCR Zeta Chain
  • TCRZCD3-ZETA

Background

T-cell surface glycoprotein CD3 zeta chain, also known as CD247, belongs to the CD3Z/FCER1G family. CD247 is T-cell receptor zeta, which together with T-cell receptor alpha/beta and gamma/delta heterodimers, and with CD3-gamma, -delta and -epsilon, forms the T-cell receptor-CD3 complex. The zeta chain plays an important role in coupling antigen recognition to several intracellular signal-transduction pathways. Low expression of the antigen results in impaired immune response. Recombinant human CD247 protein, fused to His-tag at N-terminus, was expressed in E.coli and purified by using conventional chromatography techniques.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Peptides and proteins are guaranteed for 3 months from date of receipt.

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Bioinformatics

Gene Symbol CD247