CD3 epsilon Antibody (RIV9)

Immunocytochemistry/Immunofluorescence: CD3 epsilon Antibody (RIV9) [NBP2-44880] - Immunofluorescence Analysis of methanol-fixed human tonsil cryosection stained with CF488A CD3 epsilon antibody (RIV9) (green), mounted in EverBrite Mounting Medium with DAPI (nuclei, blue).

Flow Cytometry: CD3 epsilon Antibody (RIV9) [NBP2-44880] - Flow cytometry analysis of lymphocyte-gated PBMCs unstained (gray) or stained with CF488A-labeled CD3 epsilon antibody (RIV9) (green)

Immunocytochemistry/Immunofluorescence: CD3 epsilon Antibody (RIV9) [NBP2-44880] - Immunofluorescence Analysis of Jurkat cells labeling CD3e with CD3 epsilon Antibody (RIV9) followed by Goat anti-Mouse IgG-CF488 (Green). The nuclear counterstain is Red Dot (Red).

Flow Cytometry: CD3 epsilon Antibody (RIV9) [NBP2-44880] - Flow Cytometric Analysis of Jurkat cells using CD3 epsilon Antibody (RIV9) followed by Goat anti-Mouse IgG-CF488 (Blue); Isotype Control (Red).

• Reactivity: Hu, Mu, Rt
• Applications: Flow, ICC/IF
• Clone: RIV9
• Clonality: Monoclonal
• Host: Mouse
• Conjugate: Unconjugated
• Concentration: 0.2 mg/ml

CD3 epsilon Antibody (RIV9) Summary

• Description: 200ug/ml of antibody purified from rabbit anti-serum by Protein A. Prepared in 10 mM PBS with 0.05% BSA & 0.05% azide. Also available WITHOUT BSA at 1.0 mg/ml. (NBP3-11471)
  
  Antibody with azide - store at 2 to 8C. Antibody without azide - store at -20 to -80C.
• Immunogen: Human peripheral lymphocytes (Uniprot: P07766)
• Localization: Cell surface and cytoplasmic
• Marker: T-Cell Marker
• Specificity: Recognizes the epsilon-chain of CD3, which consists of five different polypeptide chains (designated as gamma, delta, epsilon, zeta, and eta) with MW ranging from 16-28kDa. It is found on all mature T-lymphocytes, NK, T-cells, and some thymocytes. CD3, also known as T3, is a member of the immunoglobulin superfamily that plays a role in antigen recognition, signal transduction, and T cell activation. It is expressed, typically at high levels, on peripheral T cells and majority of T cell neoplasms. Thymocytes express CD3 at different level on the cell surface in the course of differentiation and, in cortical thymus, CD3 is predominantly Intracytoplasmic. This monoclonal antibody is particularly useful for induction of T cell activation in vitro and in vivo due to its unusual IgG3 isotype.
• Isotype: IgG3 Kappa
• Clonality: Monoclonal
• Host: Mouse
• Gene: CD3E
• Purity: Protein A purified

Applications/Dilutions

• Dilutions:
  • Flow Cytometry 1-2 ug/million cells
  • Immunocytochemistry/ Immunofluorescence 1-2 ug/ml
• Application Notes: Optimal dilution for a specific application should be determined.
• Theoretical MW: 20 kDa.
  Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Packaging, Storage & Formulations

• Storage: Store at 4C.
• Buffer: 10 mM PBS with 0.05% BSA
• Preservative: 0.05% Sodium Azide
• Concentration: 0.2 mg/ml
• Purity: Protein A purified

Alternate Names for CD3 epsilon Antibody (RIV9)

• CD3 epsilon
• CD3e antigen
• CD3e antigen, epsilon polypeptide (TiT3 complex)
• CD3e molecule, epsilon (CD3-TCR complex)
• CD3e
• CD3-epsilon
• FLJ18683
• T3E
• T-cell antigen receptor complex, epsilon subunit of T3
• T-cell surface antigen T3/Leu-4 epsilon chain
• T-cell surface glycoprotein CD3 epsilon chain
• TCRE

Background

CD3 epsilon is a subunit of CD3. T cell activation through the antigen receptor (TCR) involves the cytoplasmic tails of the CD3 subunits: CD3 gamma, CD3 delta, CD3 epsilon and CD3 zeta. These CD3 subunits are structurally related members of the immunoglobulins super family encoded by closely linked genes on human chromosome 11. The CD3 components have long cytoplasmic tails that associate with cytoplasmic signal transduction molecules. This association is mediated at least in part by a double tyrosine based motif present in a single copy in the CD3 subunits. CD3 may play a role in TCR induced growth arrest, cell survival and proliferation. The CD3 antigen is present on 68-82% of normal peripheral blood lymphocytes, 65-85% of thymocytes and Purkinje cells in the cerebellum. It is never expressed on B or NK cells. Decreased percentages of T lymphocytes may be observed in some autoimmune diseases.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Publications for CD3 epsilon Antibody (NBP2-44880)(1)

Publication using NBP2-44880

• Abdullah S, Ghio M, Cotton-Betteridge A et al. Succinate metabolism and membrane reorganization drives the endotheliopathy and coagulopathy of traumatic hemorrhage Science advances 2023-06-16 [PMID: 37315138] (WB, Human)

Bioinformatics

• Gene Symbol: CD3E
• Uniprot:
  • P07766()
  • P07766()