CD3 epsilon Antibody (CRIS-7)

Immunocytochemistry/Immunofluorescence: CD3 epsilon Antibody (CRIS-7) [NBP2-44878] - Immunofluorescence Analysis of PFA-fixed Jurkat cells. CD3 epsilon antibody (CRIS-7) followed by goat anti-mouse IgG-CF488 (cyan). Nuclei stained with RedDot (red).

Flow Cytometry: CD3 epsilon Antibody (CRIS-7) [NBP2-44878] - Flow cytometric analysis of lymphocyte-gated peripheral blood mononuclear cells. CD3 epsilon antibody (CRIS-7) followed goat anti-mouse IgG-CF488A (green); unstained PBMCs (gray).

Flow Cytometry: CD3 epsilon Antibody (CRIS-7) [NBP2-44878] - Flow Cytometric Analysis of Jurkat cells. CD3 epsilon Antibody (CRIS-7) followed by Goat anti-Mouse IgG-CF488 (Blue); Isotype Control (Red).

• Reactivity: Hu, Pm
• Applications: Flow, ICC/IF
• Clone: CRIS-7
• Clonality: Monoclonal
• Host: Mouse
• Conjugate: Unconjugated
• Concentration: 0.2 mg/ml

CD3 epsilon Antibody (CRIS-7) Summary

• Description: 200ug/ml of antibody purified from rabbit anti-serum by Protein A. Prepared in 10 mM PBS with 0.05% BSA & 0.05% azide. Also available WITHOUT BSA at 1.0 mg/ml. (NBP3-11448)
  
  Antibody with azide - store at 2 to 8C. Antibody without azide - store at -20 to -80 C.
• Immunogen: Stimulated human leukocytes
• Localization: Cell surface and cytoplasmic
• Marker: T-Cell Marker
• Specificity: Recognizes the epsilon-chain of CD3, which consists of five different polypeptide chains (designated as gamma, delta, epsilon, zeta, and eta) with MW ranging from 16-28kDa. The CD3 complex is closely associated at the lymphocyte cell surface with the T cell antigen receptor (TCR). Reportedly, CD3 complex is involved in signal transduction to the T cell interior following antigen recognition. The CD3 antigen is first detectable in early thymocytes and probably represents one of the earliest signs of commitment to the T cell lineage. In cortical thymocytes, CD3 is predominantly intra-cytoplasmic. However, in medullary thymocytes, it appears on the T cell surface. CD3 antigen is a highly specific marker for T cells, and is present in majority of T cell neoplasms.
• Isotype: IgG2a Kappa
• Clonality: Monoclonal
• Host: Mouse
• Gene: CD3E
• Purity: Protein A purified

Applications/Dilutions

• Dilutions:
  • Flow Cytometry 1-2 ug/million cells
  • Immunocytochemistry/ Immunofluorescence 1-2 ug/ml
• Application Notes: Induce T cell activation and proliferation: Order Ab without Azide.
  Optimal dilution for a specific application should be determined.
• Theoretical MW: 20 kDa.
  Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Packaging, Storage & Formulations

• Storage: Store at 4C.
• Buffer: 10 mM PBS with 0.05% BSA
• Preservative: 0.05% Sodium Azide
• Concentration: 0.2 mg/ml
• Purity: Protein A purified

Alternate Names for CD3 epsilon Antibody (CRIS-7)

• CD3 epsilon
• CD3e antigen
• CD3e antigen, epsilon polypeptide (TiT3 complex)
• CD3e molecule, epsilon (CD3-TCR complex)
• CD3e
• CD3-epsilon
• FLJ18683
• T3E
• T-cell antigen receptor complex, epsilon subunit of T3
• T-cell surface antigen T3/Leu-4 epsilon chain
• T-cell surface glycoprotein CD3 epsilon chain
• TCRE

Background

CD3 epsilon is a subunit of CD3. T cell activation through the antigen receptor (TCR) involves the cytoplasmic tails of the CD3 subunits: CD3 gamma, CD3 delta, CD3 epsilon and CD3 zeta. These CD3 subunits are structurally related members of the immunoglobulins super family encoded by closely linked genes on human chromosome 11. The CD3 components have long cytoplasmic tails that associate with cytoplasmic signal transduction molecules. This association is mediated at least in part by a double tyrosine based motif present in a single copy in the CD3 subunits. CD3 may play a role in TCR induced growth arrest, cell survival and proliferation. The CD3 antigen is present on 68-82% of normal peripheral blood lymphocytes, 65-85% of thymocytes and Purkinje cells in the cerebellum. It is never expressed on B or NK cells. Decreased percentages of T lymphocytes may be observed in some autoimmune diseases.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Bioinformatics

• Gene Symbol: CD3E
• Uniprot:
  • P07766()
  • P07766()