CD3 epsilon Antibody (C3e/1931)

Western Blot: CD3 epsilon Antibody (C3e/1931) [NBP2-79706] - Western Blot Analysis of human Jurkat cell lysate using CD3 epsilon Antibody (C3e/1931).

Immunohistochemistry-Paraffin: CD3 epsilon Antibody (C3e/1931) [NBP2-79706] - Formalin-fixed, paraffin-embedded human Spleen stained with CD3 epsilon Antibody (C3e/1931).

Flow Cytometry: CD3 epsilon Antibody (C3e/1931) [NBP2-79706] - Flow Cytometric Analysis of Jurkat cells. CD3 epsilon Antibody (C3e/1931) followed by Goat anti-Mouse IgG-CF488 (Blue); Isotype Control (Red).

Protein Array: CD3 epsilon Antibody (C3e/1931) [NBP2-79706] - Analysis of Protein Array containing more than 19,000 full-length human proteins using CD3 epsilon Antibody (C3e/1931) Z- and S- Score: The Z-score represents the strength of a signal that a monoclonal antibody (MAb) (in combination with a fluorescently-tagged anti-IgG secondary antibody) produces when binding to a particular protein on the HuProt(TM) array. Z-scores are described in units of standard deviations (SD's) above the mean value of all signals generated on that array. If targets on HuProt(TM) are arranged in descending order of the Z-score, the S-score is the difference (also in units of SD's) between the Z-score. S-score therefore represents the relative target specificity of a MAb to its intended target. A MAb is considered to specific to its intended target, if the MAb has an S-score of at least 2.5.

• Reactivity: Hu
• Applications: WB, ELISA, Flow, IHC, IHC-P, PA
• Clone: C3e/1931
• Clonality: Monoclonal
• Host: Mouse
• Conjugate: Unconjugated
• Concentration: 0.2 mg/ml

CD3 epsilon Antibody (C3e/1931) Summary

• Immunogen: A recombinant human CD3 epsilon protein fragment (around aa 23-119) (exact sequence is proprietary)
• Localization: Cell Surface, Cytoplasm
• Specificity: This antibody recognizes the epsilon-chain of CD3, which consists of five different polypeptide chains (designated as gamma, delta, epsilon, zeta, and eta) with MW ranging from 16-28kDa.
• Isotype: IgG1 Kappa
• Clonality: Monoclonal
• Host: Mouse
• Gene: CD3E
• Purity: Protein A or G purified

Applications/Dilutions

• Dilutions:
  • Western Blot 1-2 ug/ml
  • ELISA 2-4 ug/ml
  • Flow Cytometry 1-2 ug/million cells
  • Immunohistochemistry 1-2 ug/ml
  • Immunohistochemistry-Paraffin 1-2 ug/ml
  • Protein Array
• Application Notes: ELISA: Use Ab at 2-4ug/ml for coating. Order Ab without BSA.
  Immunohistochemistry (Formalin-fixed): 1-2ug/ml for 30 minutes at RT. Staining of formalin-fixed tissues requires heating tissue sections in 10mM Tris buffer with 1mM EDTA, pH 9.0, for 45 min at 95C followed by cooling at RT for 20 minutes.
  Optimal dilution for a specific application should be determined.

Packaging, Storage & Formulations

• Storage: Store at 4C.
• Buffer: 10 mM PBS with 0.05% BSA
• Preservative: 0.05% Sodium Azide
• Concentration: 0.2 mg/ml
• Purity: Protein A or G purified

Alternate Names for CD3 epsilon Antibody (C3e/1931)

• CD3 epsilon
• CD3e antigen
• CD3e antigen, epsilon polypeptide (TiT3 complex)
• CD3e molecule, epsilon (CD3-TCR complex)
• CD3e
• CD3-epsilon
• FLJ18683
• T3E
• T-cell antigen receptor complex, epsilon subunit of T3
• T-cell surface antigen T3/Leu-4 epsilon chain
• T-cell surface glycoprotein CD3 epsilon chain
• TCRE

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Bioinformatics

• Gene Symbol: CD3E
• Uniprot:
  • P07766()
  • P07766()