CD3 epsilon Antibody (C3e/1308) - Azide and BSA Free

Images

 
Western Blot: CD3 epsilon Antibody (C3e/1308) - Azide and BSA Free [NBP2-54392] - Western Blot Analysis of human Jurkat cell lysate using CD3 epsilon Antibody (C3e/1308).
Immunohistochemistry-Paraffin: CD3 epsilon Antibody (C3e/1308) - Azide and BSA Free [NBP2-54392] - Formalin-fixed, paraffin-embedded human Tonsil stained with CD3 epsilon Monoclonal Antibody (C3e/1308).
Flow (Intracellular): CD3 epsilon Antibody (C3e/1308) - Azide and BSA Free [NBP2-54392] - An intracellular stain was performed on Jurkat cells with CD3 epsilon Antibody (C3e/1308) NBP2-54392AF488 (blue) and a matched ...read more
Western Blot: CD3 epsilon Antibody (C3e/1308) - Azide and BSA Free [NBP2-54392] - Western Blot of recombinant CD3e and Jurkat Cell Lysate using CD3e Monoclonal Antibody (C3e/1308)
Flow Cytometry: CD3 epsilon Antibody (C3e/1308) - Azide and BSA Free [NBP2-54392] - Flow Cytometric Analysis of Jurkat cells using CD3 epsilon Antibody (C3e/1308) followed by Goat anti-Mouse IgG-CF488 (Blue); Isotype ...read more

Product Details

Summary
Reactivity HuSpecies Glossary
Applications WB, Flow, Flow-IC, ICC/IF, IHC, IHC-P, CyTOF-ready
Clone
C3e/1308
Clonality
Monoclonal
Host
Mouse
Conjugate
Unconjugated
Format
Azide and BSA Free
Concentration
1 mg/ml

Order Details

CD3 epsilon Antibody (C3e/1308) - Azide and BSA Free Summary

Immunogen
Recombinant human CD3e fragment (aa 23-119)
Localization
Cell surface and Cytoplasmic
Marker
T-Cell Marker
Isotype
IgG2b Kappa
Clonality
Monoclonal
Host
Mouse
Gene
CD3E
Purity
Protein A purified
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.

Applications/Dilutions

Dilutions
  • Western Blot 1 - 2 ug/ml
  • Flow Cytometry 0.5 - 1 ug/million cells
  • Immunocytochemistry/Immunofluorescence 1 - 2 ug/ml
  • Immunohistochemistry
  • Immunohistochemistry-Paraffin 1 - 2 ug/ml
  • CyTOF-ready
  • Flow (Intracellular)
Application Notes
IHC-P: Staining of formalin-fixed tissues requires boiling tissue sections in 10mM citrate buffer pH 6.0 for 10-20 min followed by cooling at RT for 20 minutes
Theoretical MW
20 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Packaging, Storage & Formulations

Storage
Store at -20 to -80C. Avoid freeze-thaw cycles.
Buffer
10 mM PBS
Preservative
No Preservative
Concentration
1 mg/ml
Purity
Protein A purified

Alternate Names for CD3 epsilon Antibody (C3e/1308) - Azide and BSA Free

  • CD3 epsilon
  • CD3e antigen
  • CD3e antigen, epsilon polypeptide (TiT3 complex)
  • CD3e molecule, epsilon (CD3-TCR complex)
  • CD3e
  • CD3-epsilon
  • FLJ18683
  • T3E
  • T-cell antigen receptor complex, epsilon subunit of T3
  • T-cell surface antigen T3/Leu-4 epsilon chain
  • T-cell surface glycoprotein CD3 epsilon chain
  • TCRE

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Product General Protocols

Find general support by application which include: protocols, troubleshooting, illustrated assays, videos and webinars.

Video Protocols

WB Video Protocol
ICC/IF Video Protocol

FAQs for CD3 epsilon Antibody (NBP2-54392) (0)

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Secondary Antibodies

 

Isotype Controls

Other Available Formats

Alexa Fluor 350 NBP2-54392AF350
Alexa Fluor 405 NBP2-54392AF405
Alexa Fluor 488 NBP2-54392AF488
Alexa Fluor 532 NBP2-54392AF532
Alexa Fluor 594 NBP2-54392AF594
Alexa Fluor 647 NBP2-54392AF647
Alexa Fluor 700 NBP2-54392AF700
Alexa Fluor 750 NBP2-54392AF750
Allophycocyanin NBP2-54392APC
Allophycocyanin/Cy7 NBP2-54392APCCY7
Biotin NBP2-54392B
DyLight 350 NBP2-54392UV
DyLight 405 NBP2-54392V
DyLight 488 NBP2-54392G
DyLight 550 NBP2-54392R
DyLight 594 NBP2-54392DL594
DyLight 650 NBP2-54392C
DyLight 680 NBP2-54392FR
DyLight 755 NBP2-54392IR
FITC NBP2-54392F
HRP NBP2-54392H
Janelia Fluor 549 NBP2-54392JF549
Janelia Fluor 646 NBP2-54392JF646
PE NBP2-54392PE
PE/Atto594 NBP2-54392PEATT594
PE/Cy5.5 NBP2-54392PECY55
PE/Cy7 NBP2-54392PECY7
PerCP NBP2-54392PCP

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Bioinformatics

Gene Symbol CD3E