CD3 epsilon Antibody (C363.29B (YCD3-1)) [Biotin]

Product Discontinued

CD3 epsilon Antibody (C363.29B (YCD3-1)) [Biotin] Summary

• Immunogen: IL-4 producing Th2 cell lines including D10
• Specificity: Mouse CD3 epsilon (Mr 25 kD)
• Isotype: IgG2b Kappa
• Clonality: Monoclonal
• Host: Rat
• Gene: CD3E
• Purity: Protein A or G purified

Applications/Dilutions

• Dilutions:
  • Flow Cytometry <= 3 ug/10^6 cells
  • Immunohistochemistry 1:10-1:500
  • Immunohistochemistry-Frozen 1:10-1:500
  • Immunohistochemistry-Paraffin 1:10-1:500
  • Immunoprecipitation 1:10-1:500
  • In vitro assay
• Readout System:
  • Streptavidin Native Protein NBP2-29367
  • Streptavidin Native Protein NBP2-29370
  • Streptavidin Native Protein NBP2-29371

Packaging, Storage & Formulations

• Storage: Store at 4C. Do not freeze.
• Buffer: PBS
• Preservative: 0.05% Sodium Azide
• Purity: Protein A or G purified

Notes

Protect conjugated forms from light.

Alternate Names for CD3 epsilon Antibody (C363.29B (YCD3-1)) [Biotin]

• CD3 epsilon
• CD3e antigen
• CD3e antigen, epsilon polypeptide (TiT3 complex)
• CD3e molecule, epsilon (CD3-TCR complex)
• CD3e
• CD3-epsilon
• FLJ18683
• T3E
• T-cell antigen receptor complex, epsilon subunit of T3
• T-cell surface antigen T3/Leu-4 epsilon chain
• T-cell surface glycoprotein CD3 epsilon chain
• TCRE

Background

T cell activation through the antigen receptor (TCR) involves the cytoplasmic tails of the CD3 subunits: CD3 gamma, CD3 delta, CD3 epsilon and CD3 zeta. These CD3 subunits are structurally related members of the immunoglobulins super family encoded by closely linked genes on human chromosome 11. The CD3 components have long cytoplasmic tails that associate with cytoplasmic signal transduction molecules. This association is mediated at least in part by a double tyrosine based motif present in a single copy in the CD3 subunits. CD3 may play a role in TCR induced growth arrest, cell survival and proliferation. The CD3 antigen is present on 68-82% of normal peripheral blood lymphocytes, 65-85% of thymocytes and Purkinje cells in the cerebellum. It is never expressed on B or NK cells. Decreased percentages of T lymphocytes may be observed in some autoimmune diseases.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Publications for CD3 epsilon Antibody (NBP1-26687)(5)

Publications using NBP1-26687

• WhitelJL, Nicholls SM, Shimeld C et al. Immunohistochemical detection of T-cell subsets and other leukocytes in paraffin-embedded rat and mouse tissues with monoclonal antibodies J Histochem Cytochem 1995-03-01 [PMID: 7868861]
  
  Details:
  This citation used the Biotin version of this antibody.
• Portoles P, Rojo J, Golby A et al. Monoclonal antibodies to murine CD3 epsilon define distinct epitopes, one of which may interact with CD4 during T cell activation J Immunol 1989-06-01 [PMID: 2470817]
  
  Details:
  This citation used the Biotin version of this antibody.
• Dianzani U, Luqman M, Rojo J et al. Molecular associations on the T cell surface correlate with immunological memory Eur J Immunol 1990-10-01 [PMID: 1978709]
  
  Details:
  This citation used the Biotin version of this antibody.
• Ernst DN, Hobbs MV, Torbett BE et al. Differences in the expression profiles of CD45RB, Pgp-1, and 3G11 membrane antigens and in the patterns of lymphokine secretion by splenic CD4+ T cells from young and aged mice J Immunol [PMID: 1974562]
  
  Details:
  Used the Biotin form of this antibody.
• Luqman M, Greenbaum L, Lu D et al. Differential effect of interleukin 1 on naive and memory CD4+ T cells Eur J Immunol 1992-01-01 [PMID: 1346115]
  
  Details:
  This citation used the Biotin version of this antibody.

FAQs for CD3 epsilon Antibody (NBP1-26687). (Showing 1 - 2 of 2 FAQs).

1. Which antigen retrieval or protease pretreatment is appropriate for NBP1-26687 when it was used for Formalin-Fixed, parraffin embedded (FFPE) bone marrow tissue sections?
   • Our lab routinely follows a citrate buffer method. Please see our antigen retrieval protocol.
2. What is the dilution for Immunohistochemistry-Paraffin with NBP1-26687?
   • I would recommend 1:100-1:200 as a starting dilution for IHC-P, and then you may need to optimize from there depending on your experimental conditions.

Bioinformatics

• Gene Symbol: CD3E