CD3 epsilon Antibody (C363.29B (YCD3-1)) [Biotin]

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Product Details

Summary
Reactivity MuSpecies Glossary
Applications Flow, IHC, IHC-Fr, IHC-P, In vitro, IP
Clone
C363.29B (YCD3-1)
Clonality
Monoclonal
Host
Rat
Conjugate
Biotin

Order Details

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CD3 epsilon Antibody (C363.29B (YCD3-1)) [Biotin] Summary

Immunogen
IL-4 producing Th2 cell lines including D10
Specificity
Mouse CD3 epsilon (Mr 25 kD)
Isotype
IgG2b Kappa
Clonality
Monoclonal
Host
Rat
Gene
CD3E
Purity
Protein A or G purified
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Test in a species/application not listed above to receive a full credit towards a future purchase.

Applications/Dilutions

Dilutions
  • Flow Cytometry <= 3 ug/10^6 cells
  • Immunohistochemistry 1:10-1:500
  • Immunohistochemistry-Frozen 1:10-1:500
  • Immunohistochemistry-Paraffin 1:10-1:500
  • Immunoprecipitation 1:10-1:500
  • In vitro assay
Readout System
Publications
Read Publications using NBP1-26687.

Packaging, Storage & Formulations

Storage
Store at 4C. Do not freeze.
Buffer
PBS
Preservative
0.05% Sodium Azide
Purity
Protein A or G purified

Notes

Protect conjugated forms from light.

Alternate Names for CD3 epsilon Antibody (C363.29B (YCD3-1)) [Biotin]

  • CD3 epsilon
  • CD3e antigen
  • CD3e antigen, epsilon polypeptide (TiT3 complex)
  • CD3e molecule, epsilon (CD3-TCR complex)
  • CD3e
  • CD3-epsilon
  • FLJ18683
  • T3E
  • T-cell antigen receptor complex, epsilon subunit of T3
  • T-cell surface antigen T3/Leu-4 epsilon chain
  • T-cell surface glycoprotein CD3 epsilon chain
  • TCRE

Background

T cell activation through the antigen receptor (TCR) involves the cytoplasmic tails of the CD3 subunits: CD3 gamma, CD3 delta, CD3 epsilon and CD3 zeta. These CD3 subunits are structurally related members of the immunoglobulins super family encoded by closely linked genes on human chromosome 11. The CD3 components have long cytoplasmic tails that associate with cytoplasmic signal transduction molecules. This association is mediated at least in part by a double tyrosine based motif present in a single copy in the CD3 subunits. CD3 may play a role in TCR induced growth arrest, cell survival and proliferation. The CD3 antigen is present on 68-82% of normal peripheral blood lymphocytes, 65-85% of thymocytes and Purkinje cells in the cerebellum. It is never expressed on B or NK cells. Decreased percentages of T lymphocytes may be observed in some autoimmune diseases.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Publications for CD3 epsilon Antibody (NBP1-26687)(5)

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Product General Protocols

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FAQs for CD3 epsilon Antibody (NBP1-26687). (Showing 1 - 2 of 2 FAQs).

  1. Which antigen retrieval or protease pretreatment is appropriate for NBP1-26687 when it was used for Formalin-Fixed, parraffin embedded (FFPE) bone marrow tissue sections?
    • Our lab routinely follows a citrate buffer method. Please see our antigen retrieval protocol.
  2. What is the dilution for Immunohistochemistry-Paraffin with NBP1-26687?
    • I would recommend 1:100-1:200 as a starting dilution for IHC-P, and then you may need to optimize from there depending on your experimental conditions.

Secondary Antibodies

 

Isotype Controls

Other Available Formats

Allophycocyanin Labeled NBP1-26689
Cy5 Labeled NBP1-26690
FITC Labeled NBP1-26686
PE Labeled NBP1-26688

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Bioinformatics

Gene Symbol CD3E
Entrez