Flow Cytometry: CD3 epsilon Antibody (145-2C11) [NBP2-30151] - 1 ug/1^6 BALB/c spleen cells were double-stained with hamster anti-mouse CD3 epsilon-FITC and rat anti-mouse CD19-R-PE. Lymphocytes were gated and analyzed ...read more
Flow Cytometry: CD3 epsilon Antibody (145-2C11) [NBP2-30151] - Analysis using the Sodium Azide version of NBP2-30149. Staining of Mosue splenocytes using 0.5 ug of antibody (Red represents antibody).
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CD3 epsilon Antibody (145-2C11) Summary
H-2Kb specific cytotoxic T lymphocyte clone BM10-37
Binds to lymphocytes from all mouse strains tested and does not react with cells from rats, rabbits, miniature swine, or hamsters. Binds to the CD3 epsilon -chain present on T-lymphocyes and thymocytes. Its binding has been characterized with respect to several other monoclonal anti-CD3 antibodies.
Protein G purified
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Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
PBS, 0.05% BSA
0.05% Sodium Azide
Protein G purified
Alternate Names for CD3 epsilon Antibody (145-2C11)
CD3e antigen, epsilon polypeptide (TiT3 complex)
CD3e molecule, epsilon (CD3-TCR complex)
T-cell antigen receptor complex, epsilon subunit of T3
T-cell surface antigen T3/Leu-4 epsilon chain
T-cell surface glycoprotein CD3 epsilon chain
CD3 is a multi-subunit complex consisting of 4 different invariable membrane proteins, CD3E, CD3D, CD3G and CD3-omega. These CD3 proteins are expressed on the surface of T-cells and Thymocytes during thymocyte development. They are non-covalently associated with the TCR heterodimer and form the functional TCR-CD3 antigen receptor complex of the T-lymphocyte. CD3 expression is significant during Intrathymic T-lymphocyte maturation, TCR signaling and Thymocyte differentiation. Stimulation of TCR-CD3 complex results in activation of various protein tyrosine kinases including ZAP-70 affecting multiple cellular responses including T-cell proliferation, clonal expansion, induction of tolerance and apoptosis. Mutations in the sub-unit chains of CD3 have been associated with various immunodeficiency syndromes including SCID.
This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.
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