CD3 delta Antibody (1F4)

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Immunohistochemistry-Paraffin: CD3 Antibody (1F4) [NB100-65230] - Staining of paraffin embedded rat spleen with Mouse anti Rat CD3.

Product Details

Summary
Reactivity RtSpecies Glossary
Applications Flow, IHC, IHC-Fr, IHC-P, IP
Clone
1F4
Clonality
Monoclonal
Host
Mouse
Conjugate
Unconjugated
Concentration
1.0 mg/ml

Order Details

CD3 delta Antibody (1F4) Summary

Immunogen
F344 rat T cells stimulated with PMA (TPA) and calcium ionophore
Localization
Type I membrane protein.
Specificity
Recognizes Rat CD3D (Gene ID: 25710, UniProt: P19377) and Rat CD3G (Gene ID: 300678, UniProt: Q64159) NB100-65230 recognizes rat CD3, a 25kD antigen which is found on rat T-cells. NB100-65230 does not react with rat B cells. In immunohistology it stains rat thymus tissues strongly in the medulla and weakly in the cortex. Functionally the addition of the antibody to a culture of rat T cells induces the proliferation of T-cells in the presence of PMA.
Isotype
IgM
Clonality
Monoclonal
Host
Mouse
Gene
CD3D
Purity
IgM purified
Innovator's Reward
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Applications/Dilutions

Dilutions
  • Flow Cytometry 1:10-1:25
  • Immunohistochemistry 1:10-1:500
  • Immunohistochemistry-Frozen 1:10-1:25
  • Immunohistochemistry-Paraffin 1:10
  • Immunoprecipitation 1:10-1:500

Packaging, Storage & Formulations

Storage
Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
Buffer
PBS
Preservative
0.09% Sodium Azide
Concentration
1.0 mg/ml
Purity
IgM purified

Alternate Names for CD3 delta Antibody (1F4)

  • CD3 antigen, delta subunit
  • CD3d antigen
  • CD3d antigen, delta polypeptide (TiT3 complex)
  • CD3d molecule, delta (CD3-TCR complex)
  • CD3-DELTA
  • T3DOKT3, delta chain
  • T-cell receptor T3 delta chain
  • T-cell surface glycoprotein CD3 delta chain

Background

CD3 complex is crucial in transducing antigen-recognition signals into the cytopasm of T cells and in regulating the cell surface expression of the TCR complex. T cell activation through the antigen receptor (TCR) involves the cytoplasmic tails of the CD3 subunits CD3 gamma, CD3 delta, CD3 epsilon and CD3 zeta. These CD3 subunits are structurally related members of the immunoglobulins super family encoded by closely linked genes on human chromosome 11. The CD3 components have long cytoplasmic tails that associate with cytoplasmic signal transduction molecules. This association is mediated at least in part by a double tyrosine-based motif present in a single copy in the CD3 subunits. CD3 may play a role in TCR-induced growth arrest, cell survival and proliferation. The CD3 antigen is present on 68-82% of normal peripheral blood lymphocytes, 65-85% of thymocytes and Purkinje cells in the cerebellum. It is never expressed on B or NK cells. Decreased percentages of T lymphocytes may be observed in some autoimmune diseases.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Bioinformatics

Gene Symbol CD3D