CD3 Antibody (G4.18)

Images

 
Flow Cytometry: CD3 Antibody (G4.18) [NBP1-43458] - Analysis using the Biotin conjugate of NBP1-43458. Staining of rat splenocytes with Anti-Rat CD45R (B220) FITC and staining buffer (autofluorescence) (left) or 0.125 ...read more
Flow Cytometry: CD3 Antibody (G4.18) [NBP1-43458] - Staining of rat splenocytes with 0.25 ug of Mouse IgG3 Isotype Control Purified (open histogram) or 0.25 ug of Anti-Rat CD3 Purified (filled histogram) followed by ...read more

Product Details

Summary
Reactivity RtSpecies Glossary
Applications WB, Flow, IHC, IHC-P, IP
Clone
G4.18
Clonality
Monoclonal
Host
Mouse
Conjugate
Unconjugated
Concentration
0.5 mg/ml

CD3 Antibody (G4.18) Summary

Immunogen
The immunogen for this antibody was CD3.
Specificity
The G4.18 monoclonal antibody recognizes the rat CD3 protein.
Isotype
IgG3 Kappa
Clonality
Monoclonal
Host
Mouse
Gene
CD3
Purity
Protein A or G purified
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.

Applications/Dilutions

Dilutions
  • Western Blot 1:100-1:2000
  • Flow Cytometry 1:10-1:1000
  • Immunohistochemistry 1:10-1:500
  • Immunohistochemistry-Paraffin 1:10-1:500
  • Immunoprecipitation 1:10-1:500

Packaging, Storage & Formulations

Storage
Store at 4C. Do not freeze.
Buffer
Aqueous, may contain carrier protein/stabilizer.
Preservative
0.09% Sodium Azide
Concentration
0.5 mg/ml
Purity
Protein A or G purified

Alternate Names for CD3 Antibody (G4.18)

  • CD3 antigen, delta subunit
  • CD3
  • CD3d antigen
  • CD3d antigen, delta polypeptide (TiT3 complex)
  • CD3d molecule, delta (CD3-TCR complex)
  • CD3-DELTA
  • CD3e antigen
  • CD3e antigen, epsilon polypeptide (TiT3 complex)
  • CD3e molecule, epsilon (CD3-TCR complex)
  • CD3-epsilon
  • CD3g antigen
  • CD3g antigen, gamma polypeptide (TiT3 complex)
  • CD3g molecule, epsilon (CD3-TCR complex)
  • CD3g molecule, gamma (CD3-TCR complex)
  • CD3G
  • CD3-GAMMA
  • FLJ17620
  • FLJ17664
  • FLJ18683
  • FLJ79544
  • FLJ94613
  • MGC138597
  • T3DOKT3, delta chain
  • T3E
  • T-cell antigen receptor complex, epsilon subunit of T3
  • T-cell receptor T3 delta chain
  • T-cell surface antigen T3/Leu-4 epsilon chain
  • T-cell surface glycoprotein CD3 delta chain
  • T-cell surface glycoprotein CD3 epsilon chain
  • TCRE

Background

The G4.18 monoclonal antibody recognizes the rat CD3 protein. CD3 is a critical component of the T-cell receptor (TCR) and is a marker of the T-cell lineage. CD3 is a complex of several subunits and, upon binding of a TCR ligand, participates in transduction of signals from the TCR to the nucleus which results in a variety of cellular responses including transcription of IL-2 and clonal expansion. in vitro, immobilized G4.18 monoclonal antibody has been demonstrated to activate T cells, while soluble G4.18 inhibited allogeneic mixed-lymphocyte proliferative responses and cell-mediated cytotoxicity to allogeneic target cells. in vivo, G4.18 was demonstrated to induce long-term specific tolerance to an organ allograft.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Product General Protocols

Find general support by application which include: protocols, troubleshooting, illustrated assays, videos and webinars.

Video Protocols

WB Video Protocol

FAQs for CD3 Antibody (NBP1-43458). (Showing 1 - 1 of 1 FAQ).

  1. What the theoretical molecular weight for CD3 antibodies?
    • The TMW of CD3 antibodies is approximately 20 - 25 kDa.

Secondary Antibodies

 

Isotype Controls

Other Available Formats

Alexa Fluor 405 Labeled NBP1-43458AF405
Alexa Fluor 488 Labeled NBP1-43458AF488
Alexa Fluor 647 Labeled NBP1-43458AF647
Alexa Fluor 700 Labeled NBP1-43458AF700
Allophycocyanin Labeled NBP1-43458APC
Biotin Labeled NBP1-43693
DyLight 350 Labeled NBP1-43458UV
DyLight 405 Labeled NBP1-43458V
DyLight 488 Labeled NBP1-43458G
DyLight 680 Labeled NBP1-43458FR
DyLight 755 Labeled NBP1-43458IR
PE Labeled NBP1-43458PE
PerCP Labeled NBP1-43458PCP

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Bioinformatics

Gene Symbol CD3
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