CD2AP Antibody

Western blot shows lysates of MOLT-4 human acute lymphoblastic leukemia cell line, Ramos human Burkitt's lymphoma cell line, KG-1 human acute myelogenous leukemia cell line, M1 mouse myeloid leukemia cell line, and Y3-Ag rat myeloid cell line. PVDF membrane was probed with 0.2 µg/mL of Human/Mouse/Rat CD2AP Antigen Affinity-purified Polyclonal Antibody (Catalog # AF4474) followed by HRP-conjugated Anti-Sheep IgG Secondary Antibody (Catalog # HAF016). A specific band was detected for CD2AP at approximately 80 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.

• Reactivity: Hu, Mu, Rt
• Applications: WB
• Clonality: Polyclonal
• Host: Sheep
• Conjugate: Unconjugated
• Concentration: LYOPH

CD2AP Antibody Summary

• Immunogen: E. coli-derived recombinant human CD2AP
  Ile423-Lys580
  Accession # Q9Y5K6
• Specificity: Detects endogenous human, mouse, and rat CD2AP in Western blots.
• Source: N/A
• Isotype: IgG
• Clonality: Polyclonal
• Host: Sheep
• Gene: CD2AP
• Purity Statement: Antigen Affinity-purified

Applications/Dilutions

• Dilutions:
  • Western Blot 0.2 ug/mL

Packaging, Storage & Formulations

• Storage: Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.
• Buffer: Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
• Preservative: No Preservative
• Concentration: LYOPH
• Reconstitution Instructions: Reconstitute at 0.2 mg/mL in sterile PBS.

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for CD2AP Antibody

• adapter protein CMS
• Cas ligand with multiple Src homology 3 (SH3) domains
• CD2AP
• CD2-associated protein
• CMS
• DKFZp586H0519

Background

CD2AP (CD2-associated protein; also known as CMS) is an 80 kDa member of the CIN85/CD2AP family of adaptor proteins. Although CD2AP is ubiquitously expressed, it is often associated with glomerular podocytes. CD2AP serves as a scaffold between membrane proteins and the cytoskeleton. Through a Pro-rich region and SH3 domains, it is known to bind to such proteins as CD2, c-CBL, and p130^Cas. Human CD2AP is 639 amino acids (aa) in length. It contains three SH3 homology domains (aa 4‑324), one Pro-rich region (aa 336‑422) and a C-terminal coiled-coil region that mediates homodimerization. Over aa 423‑580, human CD2AP shares 96% and 79% aa identity with dog and mouse CD2AP, respectively.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Publications for CD2AP Antibody (AF4474)(3)

Publications using AF4474

• LE Wong, A Bhatt, PS Erdmann, Z Hou, J Maier, S Pirkuliyev, M Engelke, S Becker, J Plitzko, J Wienands, C Griesinger Tripartite phase separation of two signal effectors with vesicles priming B cell responsiveness Nat Commun, 2020-02-12;11(1):848. 2/12/2020 [PMID: 32051419]
• Hiroshi Nakamoto Visualisation studies and glomerular filtration in early diabetic rats J Biomech, 2016-11-12;0(0):. 2016-11-12 [PMID: 27887726] (IHC, Rat)
• Niiro H, Jabbarzadeh-Tabrizi S, Kikushige Y, Shima T, Noda K, Ota S, Tsuzuki H, Inoue Y, Arinobu Y, Iwasaki H, Shimoda S, Baba E, Tsukamoto H, Horiuchi T, Taniyama T, Akashi K CIN85 is required for Cbl-mediated regulation of antigen receptor signaling in human B cells. Blood, 2012-01-18;119(10):2263-73. 2012-01-18 [PMID: 22262777] (Immunoprecipitation, Human)

Bioinformatics

• Gene Symbol: CD2AP
• Uniprot:
  • Q9Y5K6(Human)