CD28 Antibody (YTH913.12)

Images

 
Flow Cytometry: CD28 Antibody (YTH913.12) [NB100-64867] - Analysis using the PE conjugate of NB100-64867. Staining of human peripheral blood lymphocytes.
Flow Cytometry: CD28 Antibody (YTH913.12) [NB100-64867] - Staining of human peripheral blood lymphocytes with Rat anti Human.
Flow Cytometry: CD28 Antibody (YTH913.12) [NB100-64867] - Staining of human peripheral blood lymphocytes with Rat anti Human.
Flow Cytometry: CD28 Antibody (YTH913.12) [NB100-64867] - Analysis using the Biotin conjugate of NB100-64867. Staining of human peripheral blood lymphocytes.

Product Details

Summary
Reactivity HuSpecies Glossary
Applications Flow, IHC, IHC-Fr
Clone
YTH913.12
Clonality
Monoclonal
Host
Rat
Conjugate
Unconjugated
Concentration
1 mg/ml

CD28 Antibody (YTH913.12) Summary

Immunogen
Human peripheral blood T-cells
Specificity
NB100-64867 recognizes the human CD28 cell surface antigen, a 44kD glycoprotein expressed by a subset of T lymphocytes. The antibody enhances the MLR response. It has been reported that this clone also recognizes an epitope of CD28 expressed by NK cells, which is not recognized by clones of 9.3 or CD28.2.(2)
Isotype
IgG2b
Clonality
Monoclonal
Host
Rat
Gene
CD28
Purity
Protein G purified
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Applications/Dilutions

Dilutions
  • Flow Cytometry 1:25-1:50
  • Immunohistochemistry 1:10-1:500
  • Immunohistochemistry-Frozen 1:10-1:500

Packaging, Storage & Formulations

Storage
Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
Buffer
Phosphate buffered saline
Preservative
0.09% Sodium Azide
Concentration
1 mg/ml
Purity
Protein G purified

Alternate Names for CD28 Antibody (YTH913.12)

  • CD28 antigen (Tp44)
  • CD28 antigen
  • CD28 molecule
  • CD28
  • MGC138290
  • T-cell-specific surface glycoprotein CD28
  • Tp44

Background

Human CD28 antigen is a 44 kDa disulfide linked homodimeric T cell specific surface glycoprotein. It is a cell adhesion molecule of the immunoglobulin superfamily which is constitutively expressed on most peripheral blood T lymphocytes (approximately 95% of CD4+ cells and 50% of CD8+ cells). Mature thymocytes exhibit higher levels of CD28 than the immature cells. Activation of T cells results in enhanced CD28 expression. T cell activation requires two combined signals provided by antigen presenting cells. The first is mediated via the T cell receptor following its interaction with antigenic peptide MHC complexes, and the second is delivered by accessory or costimulating molecules through their counter receptors on T lymphocytes. CD28 bears structural homology to CTLA 4 which is expressed at very low levels on the surface of CD4+ and CD8+ peripheral blood cells only following activation. CD28 is the natural receptor for the B7/BB 1 ligand (CD80) a 55-60 kDa glycoprotein which is expressed on activated B lymphocytes, on dendritic cells and on interferon gamma treated monocytes. The binding of B7 1/BB 1 molecules to CD28 is involved in T lymphocyte activation and in the initiation and maintenance of chronic inflammation.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Secondary Antibodies

 

Isotype Controls

Other Available Formats

PE Labeled NB100-63658

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Blogs on CD28.

Thomson Reuters Predicts 2016 Nobel Prize Winners
Here at Bio-Techne we always look forward to the annual announcements of winners of the highly coveted Nobel Prize – the greatest award in science. How can you go about predicting which scientists might be in line for a life-changing phone-call fro...  Read full blog post.

CD80: A co-stimulator of T cell activation
CD80 is a 60kD single chain type I transmembrane glycoprotein that is a member of the immunoglobulin family. CD80 is expressed on activated B- and T-lymphocytes, as well as a subpopulation of previously activated B-cells, but not on the majority of re...  Read full blog post.

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Bioinformatics

Gene Symbol CD28
Entrez