CD25/IL-2R alpha Antibody (745520) [Phycoerythrin]

• Applications: Flow
• Clone: 745520
• Clonality: Monoclonal
• Host: Mouse
• Conjugate: Phycoerythrin

CD25/IL-2R alpha Antibody (745520) [Phycoerythrin] Summary

• Immunogen: Chinese hamster ovary cell line CHO-derived recombinant rat IL-2 R alpha
  Glu22-Gln235
  Accession # P26897
• Specificity: Detects rat IL-2 R alpha in ELISAs. In direct ELISAs, no cross-reactivity with recombinant mouse (rm) IL-2 R alpha, rmIL-2 R beta, rmCommon gamma chain, rmIL-15 R alpha, or recombinant human IL-2 R alpha is observed.
• Source: N/A
• Isotype: IgG1
• Clonality: Monoclonal
• Host: Mouse
• Gene: IL2RA
• Purity Statement: Protein A or G purified from hybridoma culture supernatant

Packaging, Storage & Formulations

• Storage: Store the unopened product at 2 - 8° C. Do not use past expiration date. Protect from light.
• Buffer: Supplied in a saline solution containing BSA and Sodium Azide.
• Preservative: Sodium Azide

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Background

IL-2 receptor alpha (IL-2 R alpha ), also known as CD25, is a 55 kDa type I transmembrane (TM) glycoprotein that functionally belongs to a family of cytokine receptors that utilize the common gamma chain subunit ( gamma _c) (1-3). IL-2 R alpha is expressed on activated T cells, and on regulatory T cells (Treg), mast cells, fibroblasts, NK cells, endthelial cells and pre-B cells (4-6). The rat IL-2 R alpha cDNA encodes a 267 amino acid (aa) precursor that includes a 21 aa signal peptide, a 214 aa extracellular domain (ECD) with two Sushi domains, a 21 aa transmembrane segment, and an 11 aa cytoplasmic domain (7). Within the ECD, rat IL-2 R alpha shares 58% and 81% aa sequence identity with human and mouse IL-2 R alpha , respectively. It shares approximately 15% aa sequence identity with IL-4, -7, -9, -15, and -21 receptor subunits that also complex with gamma _c. By itself, IL-2 R alpha binds IL-2 with low affinity. It then associates with IL-2 R beta and subsequently to gamma _c to generate a ternary high affinity IL-2 receptor complex. Notably, it would appear that IL-2 R alpha also plays a role in the formation of supramolecular membrane complexes that also include MHC-II molecules and additional gamma _c -related alpha -chains (8).  A soluble form of IL-2 R alpha can be generated by proteolytic cleavage of the cell surface receptor, rendering the T cell unresponsive to IL-2 (9, 10). Increased serum levels of soluble IL-2 R alpha are found in some cancers and immune disorders (11). IL-2 R alpha is required for activation induced cell death (AICD) of naive T cells, a mechanism responsible for deleting autoreactive T cell clones (12, 13). IL-2 R alpha is also required for the development of CD4⁺CD25⁺ Treg which suppress autoreactive CD4⁺ T cells, thereby contributing to peripheral T cell homeostasis (12-15).

1. Bodnar, A. et al. (2008) Immunol. Lett. 116:117.
2. Kovanen, P.E. and W.J. Leonard (2004) Immunol. Rev. 202:67.
3. Bluestone, J.A. and Q. Tang (2005) Curr. Opin. Immunol. 17:638.
4. Alvarez-Twose, I. et al. (2012) Int. J. Lab. Hematol. 116:117. 
5. Letourneau, S. et al. (2009) J. Allergy Clin. Immunol. 123:758.  
6. Malek, T.R. and I. Castro (2010) Immunity 33:153. 
7. Page, T.H. and M.J. Dallman (1991) Eur. J. Immunol. 21:2133.
8. Nizsaloczki, E. et al. (2014) Chempsychem. 15:3969.  
9. Wagner, D.K. et al. (1986) J. Immunol. 137:592.
10. Schulz, O. et al. (1998) J. Exp. Med. 187:271.
11. Witkowska, A.M. (2005) Mediators Inflamm. 2005:121.
12. Willerford, D.M. et al. (1995) Immunity 3:521.
13. Van Parijs, L. et al. (1997) J. Immunol. 158:3738.
14. Almeida, A.R.M. et al. (2002) J. Immunol. 169:4850.
15. Bayer, A.L. et al. (2013) Immunol. Res. 57:197.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Bioinformatics

• Gene Symbol: IL2RA