CD21 Antibody (7D6) - Azide and BSA Free

Immunohistochemistry: CD21 Antibody (7D6) [DDX0120P-100] - mAb 7D6 staining on FDC clusters. Slides were incubated with mAb 7D6 and subsequently with a sheep anti-mouse IgG1. The binding of antibody was revealed using APAAP method and developed by Fast red substrate.

Immunohistochemistry: CD21 Antibody (7D6) [DDX0120P-100] - Giemsa staining of isolated FDCs clusters

• Reactivity: Hu
• Applications: IHC
• Clone: 7D6
• Clonality: Monoclonal
• Host: Mouse
• Conjugate: Unconjugated
• Format: Azide and BSA Free
• Concentration: 0.5 mg/ml

CD21 Antibody (7D6) - Azide and BSA Free Summary

• Immunogen: CD21 long isoform was used as immunogen.
• Marker: Histiocytic Marker
• Isotype: IgG1
• Clonality: Monoclonal
• Host: Mouse
• Gene: CR2
• Purity: Ion exchange chromatography

Applications/Dilutions

• Dilutions:
  • Immunohistochemistry 1:10-1:500
  • Immunohistochemistry-Frozen 1:10-1:500

Packaging, Storage & Formulations

• Storage: Store at -20C. Avoid freeze-thaw cycles.
• Buffer: Tris-NaCl (pH 8.0)
• Preservative: No Preservative
• Concentration: 0.5 mg/ml
• Purity: Ion exchange chromatography

Notes

This product is manufactured by Eurobio Scientific (formerly Dendritics) and distributed by Novus Biologicals.

Alternate Names for CD21 Antibody (7D6) - Azide and BSA Free

• C3DR
• C3DRSLEB9
• CD21 antigen
• CD21
• Complement C3d receptor
• complement component (3d/Epstein Barr virus) receptor 2
• complement receptor type 2
• CR2
• EBV receptor
• Epstein-Barr virus receptor

Background

CD21 (complement receptor 2, CR2) binds C3 complement fragments, especially its breakdown fragments, which remain covalently attached to complement activating surfaces or antigen. CD21 has important roles in uptake and retention of immunocomplexes, survival of memory B cells and in development and maintenance of the humoral response to T-dependent antigens. CD21 also serves as a key receptor for Epstein-Barr virus binding and is involved in targeting prions to folicular dendritic cells and expediting neuroinvasion following peripheral exposure to prions. A soluble form of the CD21 (sCD21) is shed from the lymphocyte surface and retains its ability to bind respective ligands.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Publications for CD21 Antibody (DDX0120P-100)(3)

Publications using DDX0120P-100

• Estes et al. Follicular dendritic cell regulation of CXCR4-mediated germinal center CD4 T cell migration. J Immunol 2004-11-01 [PMID: 15528354]
• Lindhout E et al. Fibroblast-like synoviocytes from rheumatoid arthritis patients have intrinsic properties of follicular dendritic cells. J Immunol 1999-05-01 [PMID: 10229832]
• Liu YJ et al. Follicular dendritic cells specifically express the long CR2/CD21 isoform. J Exp Med 1997-01-01 [PMID: 8996252]

Bioinformatics

• Gene Symbol: CR2