Orthogonal Strategies: Immunohistochemistry-Paraffin: CD200/OX2 Antibody [NBP1-87738] - Analysis in human cerebral cortex and liver tissues. Corresponding CD200 RNA-seq data are presented for the same tissues.
Immunohistochemistry-Paraffin: CD200/OX2 Antibody [NBP1-87738] - Staining of human cerebral cortex shows strong cytoplasmic positivity in neuronal cells.
Immunohistochemistry-Paraffin: CD200/OX2 Antibody [NBP1-87738] - Staining of human placenta shows moderate to strong membranous positivity in trophoblastic cells.
Immunohistochemistry-Paraffin: CD200/OX2 Antibody [NBP1-87738] - Staining of human tonsil shows moderate to strong membranous positivity in lymphoid cells.
Immunohistochemistry-Paraffin: CD200/OX2 Antibody [NBP1-87738] - Staining of human liver shows no positivity in hepatocytes as expected.
This antibody was developed against Recombinant Protein corresponding to amino acids: EREQLYTPASLKCSLQNAQEALIVTWQKKKAVSPENMVTFSENHGVVIQPAYKDKINITQLGLQNSTITFWNITLEDEGCYMCLFNTFGFGKISGTACLTVYVQPIVSLHYKFSEDHLNITCSATARPAPMVFWKVPRS
Specificity of human, primate CD200/OX2 antibody verified on a Protein Array containing target protein plus 383 other non-specific proteins.
Immunogen affinity purified
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FAQs for CD200/OX2 Antibody (NBP1-87738). (Showing 1 - 1 of 1 FAQ).
We are interested in using your CD200 antibody on canine skin sections (paraffin fixed) for IHC. When comparing the human epitope with the dog's protein, there is 76% ident. Do you as an antibody manufacturer have any experience if this percentage is enough for an antibody to recognize a protein?
Generally we recommend that an immunogen share over 80-85% similarity but having taken a look at the immunogen aligned with the dog sequence, there are several stretches of high similarity. As NBP1-87738 is a polyclonal, there will almost certainly be antibodies in the vial that will cross-react with dog. You may need to use this antibody at a higher concentration than is recommended, but there is a high chance that this antibody will cross-react.
The concentration calculator allows you to quickly calculate the volume, mass or concentration of your vial. Simply enter your mass, volume, or concentration values for your reagent and the calculator will determine the rest.
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