| Reactivity | RtSpecies Glossary |
| Applications | WB, Flow, IHC, IHC-Fr |
| Clone | OX-102 |
| Clonality | Monoclonal |
| Host | Mouse |
| Conjugate | DyLight 755 |
| Immunogen | Membrane fraction of thioglycollate-elicited rat peripheral cells. |
| Localization | Membrane; single pass type I membrane protein. |
| Specificity | NB100-65603 recognizes the rat OX2 (CD200) receptor 1. This antigen is a heavily glycosylated 60-100kD cell surface molecule expressed by cells of the myeloid lineage but not by T or B lymphocytes. This antibody has been shown to block the interaction of OX2 receptor 1 with CD200. |
| Isotype | IgG1 |
| Clonality | Monoclonal |
| Host | Mouse |
| Gene | CD200 |
| Purity | Protein G purified |
| Innovator's Reward | Test in a species/application not listed above to receive a full credit towards a future purchase. |
| Dilutions |
|
| Application Notes | Optimal dilution of this antibody should be experimentally determined. |
| Storage | Store at 4C in the dark. |
| Buffer | 50mM Sodium Borate |
| Preservative | 0.05% Sodium Azide |
| Purity | Protein G purified |
![CD200/OX2 Antibody (OX-102) [DyLight 755]](/sites/all/modules/enterprise-tech/et_datasheets/images/novus_guarantee.png "CD200/OX2 Antibody (OX-102) [DyLight 755]")
Secondary Antibodies |
Isotype Controls |
| Alexa Fluor 405 | NB100-65603AF405 | |
| Alexa Fluor 488 | NB100-65603AF488 | |
| Alexa Fluor 647 | NB100-65603AF647 | |
| Alexa Fluor 700 | NB100-65603AF700 | |
| Allophycocyanin | NB100-65603APC | |
| Biotin | NB100-65603B | |
| DyLight 350 | NB100-65603UV | |
| DyLight 405 | NB100-65603V | |
| DyLight 488 | NB100-65603G | |
| DyLight 550 | NB100-65603R | |
| DyLight 650 | NB100-65603C | |
| DyLight 680 | NB100-65603FR | |
| DyLight 755 | NB100-65603IR | |
| FITC | NB100-65603F | |
| HRP | NB100-65603H | |
| PE | NB100-65603PE | |
| PerCP | NB100-65603PCP |
Diseases for CD200/OX2 Antibody (NB100-65603IR)Discover more about diseases related to CD200/OX2 Antibody (NB100-65603IR).
| Pathways for CD200/OX2 Antibody (NB100-65603IR)View related products by pathway.
|
PTMs for CD200/OX2 Antibody (NB100-65603IR)Learn more about PTMs related to CD200/OX2 Antibody (NB100-65603IR).
|
The concentration calculator allows you to quickly calculate the volume, mass or concentration of your vial. Simply enter your mass, volume, or concentration values for your reagent and the calculator will determine the rest.
| Gene Symbol | CD200 |
![Western Blot: NOD2 Antibody (2D9) [NB100-524] - HCMV infection induces NOD2 mRNA and protein in HFFs and U373 cells. E. U373 glioma cells were infected with HCMV Towne strain and levels of NOD1, NOD2 and GAPDH mRNAs were measured by qRT-PCR at indicated time points. F. HFFs were infected with HCMV (Towne) at MOI of 1 PFU/cell and levels of NOD2 protein and B-actin were determined 48 and 72 hpi. G. HFFs were infected with HCMV (Towne) strain at MOI of 0.03 or 3 PFU/cell and levels of NOD2 protein and B-actin were determined at 48 hpi. Quantitative data represent mean values (+/-SD) of triplicate determinations from three independent experiments (*p<0.05, **p<0.01, ***p<0.001, one-way ANOVA test). Image collected and cropped by CiteAb from the following publication (//doi.org/10.1371/journal.pone.0092704.g001) licensed under a CC-BY licence.](http://images.novusbio.com/images2/NOD2-Antibody-(2D9)-Western-Blot-NB100-524-img0018.jpg)
![Western Blot: NOD2 Antibody (2D9) [NB100-524] - Total protein from human THP1 and US-OS cells and mouse Raw264.7 cells was separated on a 7.5% gel by SDS-PAGE, transferred to PVDF membrane and blocked in 5% non-fat milk in TBST. The membrane was probed with 2.0 ug/ml anti-NOD2 in 1% non-fat milk in TBST and detected with an anti-mouse HRP secondary antibody using chemiluminescence.](http://images.novusbio.com/images2/NOD2-Antibody-(2D9)-Western-Blot-NB100-524-img0013.jpg)
![Immunohistochemistry-Frozen: NOD2 Antibody (2D9) [NB100-524] - Overlay of NOD2-DyLight 488 (green) with phase contrast of murine colon. Image from verified customer review.](http://images.novusbio.com/images2/NOD2-Antibody-(2D9)-Immunohistochemistry-Frozen-NB100-524-img0016.jpg)
![Flow Cytometry: HLA DQ Antibody (SPV-L3) [NBP2-45041] - Flow Cytometric Analysis of Raji cells. HLA DQ Antibody (SPV-L3) followed by goat anti-Mouse IgG-CF488 (Blue); Isotype Control (Red).](http://images.novusbio.com/images2/HLA DQ Antibody (SPV-L3)-Flow Cytometry-NBP2-45041-img0001.jpg)
![Knockout Validated: B7-2/CD86 Antibody (BU63) [NBP2-25208] - Western blot shows lysates of Ramos human Burkitt's lymphoma parental cell line and B7-2 knockout (KO) Ramos cell line. PVDF membrane was probed with 1.0 ug/mL of Mouse Anti-Human B7-2 Monoclonal Antibody (Catalog # NBP2-25208) followed by HRP-conjugated Anti-Mouse IgG Secondary Antibody (Catalog #HAF018). Specific band was detected for B7-2 at approximately 90 kDa (as indicated) in the parental Ramos cell line, but is not detectable in the knockout Ramos cell line. This experiment was conducted under reducing conditions.](http://images.novusbio.com/images2/B7-2-CD86-Antibody-(BU63)-Knockout-Validated-NBP2-25208-img0005.jpg)
![Immunocytochemistry/Immunofluorescence: B7-2/CD86 Antibody (BU63) [NBP2-25208] - Immunofluorescence staining of PFA-fixed Ramos cells using B7-2/CD86 Antibody (BU63) followed by goat anti-Mouse IgG conjugated to CF488 (green). Nuclei are stained with Red Dot.](http://images.novusbio.com/images2/B7-2-CD86-Antibody-(BU63)-Immunocytochemistry-Immunofluorescence-NBP2-25208-img0006.jpg)
![Immunohistochemistry-Paraffin: B7-2/CD86 Antibody (BU63) [NBP2-25208] - Formalin-fixed, paraffin-embedded esophagus tumor tissue stained with CD86 antibody (5 ug/ml), peroxidase-conjugate and DAB chromogen. Note strong staining of differentiated squamous cells. TMA was used for this test.](http://images.novusbio.com/images2/B7-2-CD86-Antibody-(BU63)-Immunohistochemistry-Paraffin-NBP2-25208-img0004.jpg)
![Immunohistochemistry-Paraffin: Cytokeratin 15 Antibody [NBP1-85602] - Analysis in human skin and kidney tissues. Corresponding KRT15 RNA-seq data are presented for the same tissues.](http://images.novusbio.com/images2/Cytokeratin-15-Antibody-Immunohistochemistry-Paraffin-NBP1-85602-img0012.jpg)
![Western Blot: Cytokeratin 15 Antibody [NBP1-85602] - Analysis in human cell line A-431 and human cell line HEK 293.](http://images.novusbio.com/images2/Cytokeratin-15-Antibody-Western-Blot-NBP1-85602-img0011.jpg)
![Immunocytochemistry/Immunofluorescence: Cytokeratin 15 Antibody [NBP1-85602] - Staining of human cell line HaCaT shows localization to nucleoplasm & intermediate filaments. Antibody staining is shown in green.](http://images.novusbio.com/images2/Cytokeratin-15-Antibody-Immunocytochemistry-Immunofluorescence-NBP1-85602-img0006.jpg)
![Western Blot: B7-1/CD80 Antibody (62N3G8) [NBP2-25255] - Analysis in human Raji lymphoma cells.](http://images.novusbio.com/images2/B7-1-CD80-Antibody-(62N3G8)-Western-Blot-NBP2-25255-img0010.jpg)
![Immunohistochemistry-Paraffin: B7-1/CD80 Antibody (62N3G8) [NBP2-25255] - Human thymus stained with CD80 antibody at 5 ug/ml using peroxidase-conjugate and DAB chromogen. Note membrane and cytoplasmic staining.](http://images.novusbio.com/images2/B7-1-CD80-Antibody-(62N3G8)-Immunohistochemistry-Paraffin-NBP2-25255-img0002.jpg)
![Flow Cytometry: B7-1/CD80 Antibody (62N3G8) [NBP2-25255] - Raji lymphoma cells. Image from verified customer review.](http://images.novusbio.com/images2/B7-1-CD80-Antibody-(62N3G8)-Flow-Cytometry-NBP2-25255-img0009.jpg)
![Immunohistochemistry: CD11b Antibody [NB110-89474] - Staining of mouse pancreas using [NB110-89474] at 1:400 dilution. Nuclei counterstained with 4',6-diamidino-2-phenylindole (DAPI) (blue). Image from product review by verified customer.](http://images.novusbio.com/images2/CD11b-Antibody-Immunocytochemistry-Immunofluorescence-NB110-89474-img0010.jpg)
![Dual RNAscope ISH-IHC: CD11b Antibody [NB110-89474] - Formalin-fixed paraffin-embedded tissue sections of human lymph node were probed for CD11b mRNA (ACD RNAScope Probe, catalog #555098; Fast Red chromogen, ACD catalog # 322750). Adjacent tissue section was processed for immunohistochemistry using Rabbit Polyclonal (Novus Biologicals catalog #NB110-89474) at 1:50 dilution with 1 hour incubation at room temperature followed by incubation with anti-rabbit IgG VisUCyte HRP Polymer Antibody (Catalog # VC003) and DAB chromogen (yellow-brown). Tissue was counterstained with hematoxylin (blue). Specific staining was localized to lymphocytes.](http://images.novusbio.com/images2/CD11b-Antibody-Dual-RNAscope-ISH-IHC-NB110-89474-img0025.jpg)
![Immunohistochemistry: CD11b Antibody [NB110-89474] - Glibenclamide and Abcc8-/- suppress immune cell infiltration in EAE. White matter of lumbar spinal cord sections from WT control (a), untreated pid-30 WT/EAE (b), glibenclamide-treated pid-30 WT/EAE (c), and pid-30 Abcc8-/-/EAE (d) mice, stained with H&E or immunolabeled for CD45 (leucocyte), CD3 (T cells), CD20 (B cells), or CD11b (macrophage/microglia), as indicated; original magnification, x200 (H&E) or x400 (all immunolabelings). eleft panel: percent of quadrants with inflammatory cells on H&E; four mice/group. efour right panels: Quantification of CD-45-, CD3-, CD20-, and CD11b-expressing cells in white matter; four mice/group; ##P < 0.01 with respect to WT control; **P < 0.01, and ***P < 0.001 with respect to WT/EAE; scale bars, 100 um Image collected and cropped by CiteAb from the following publication (http://www.jneuroinflammation.com/content/12/1/210) licensed under a CC-BY licence.](http://images.novusbio.com/images2/CD11b-Antibody-Immunohistochemistry-NB110-89474-img0024.jpg)
![Immunohistochemistry-Paraffin: FoxP3 Antibody [NB100-39002] - Mouse spleen stained with FoxP3 antibody. IHC-P image submitted by a verified customer review.](http://images.novusbio.com/images2/FoxP3-Antibody-Immunohistochemistry-Paraffin-NB100-39002-img0011.jpg)
![Immunohistochemistry-Paraffin: FoxP3 Antibody [NB100-39002] - Tissue section of human tonsil using 1:200 dilution of rabbit anti-FoxP3 antibody. The staining was developed with HRP-labeled anti-rabbit IgG secondary antibody and DAB reagent, and nuclei of cells were counter-stained with hematoxylin. This FoxP3 antibody generated an expected and a specific nuclear staining of FOXP3 in a subset of cells which are potentially the Treg cells.](http://images.novusbio.com/images2/FoxP3-Antibody-Immunohistochemistry-Paraffin-NB100-39002-img0017.jpg)
![Immunohistochemistry: FoxP3 Antibody [NB100-39002] - Antibody was tested in mouse spleen using DAB with hematoxylin counterstain.](http://images.novusbio.com/images2/FoxP3-Antibody-Immunohistochemistry-NB100-39002-img0014.jpg)
![Western Blot: MyoD Antibody (5.8A) [NB100-56511] - Analysis for MyoD expression in various small round cell tumor lines using 1 ug/mL anti-MyoD mAb. The antibody only reacts with a band of approx. 45 kDa in the rhabdomyosarcoma cell line (Rh30, lane 1) but was negative against the primitive neuroectodermal (PFSK-1A, lane 2), lymphoma (EB2, lane 3), neuroblastoma (SK-N-SH, lane 4), and Ewing's sarcoma (SJSA-1, lane 5) cell lines.](http://images.novusbio.com/images2/MyoD-Antibody-(5.8A)-Western-Blot-NB100-56511-img0006.jpg)
![Immunocytochemistry/Immunofluorescence: MyoD Antibody (5.8A) [NB100-56511] - Human myoblasts were stained with MyoD1 antibody, diluted 1:200. ICC/IF image submitted by a verified customer review.](http://images.novusbio.com/images2/MyoD-Antibody-(5.8A)-Immunocytochemistry-Immunofluorescence-NB100-56511-img0008.jpg)
![Immunohistochemistry-Frozen: MyoD Antibody (5.8A) [NB100-56511] - Clone 5.8A antibody in human tissues. A. Rhabdomyosaroma (nuclei are stained), B. Lymphoma (staining is absent)](http://images.novusbio.com/images2/MyoD-Antibody-(5.8A)-Immunohistochemistry-NB100-56511-img0005.jpg)
![Western Blot: Doublecortin Antibody (3E1) [NBP1-92684] - Analysis of rat whole brain lysates using mouse mAb to Doublecortin (DCX), NBP1-92684, dilution 1:1,000 in green: [1] protein standard (red), [2] adult rat brain, [3] embryonic E20 rat brain. Strong bands at 40, 45kDa correspond to DCX protein, detected only in the developing brain.](http://images.novusbio.com/images2/Doublecortin-Antibody-(3E1)-Western-Blot-NBP1-92684-img0005.jpg)
![Immunocytochemistry/Immunofluorescence: Doublecortin Antibody (3E1) [NBP1-92684] - Analysis of cortical neuron-glial cell culture from E20 rat stained with mouse mAb to Doublecortin (DCX), NBP1-92684, dilution 1:1,000 in red, and costained with chicken pAb to Microtubule Associated Protein2 (MAP2), dilution 1:10,000 in green. The blue is DAPI staining of nuclear DNA. DCX antibody reveals strong cytoplasmic staining in a population of small developing neurons and their processes, while MAP2 antibody stains dendrites and perikarya of mature neurons. Therefore DCX antibody is an excellent marker of developing neuronal cells.](http://images.novusbio.com/images2/Doublecortin-Antibody-(3E1)-Immunocytochemistry-Immunofluorescence-NBP1-92684-img0004.jpg)
![Immunocytochemistry/Immunofluorescence: Doublecortin Antibody (3E1) [NBP1-92684] - Rat brain neural cultures stained with NBP1-92684 (green), a chicken polyclonal antibody to GFAP (NB300-213, red) and DNA (blue). The NBP1-92684 antibody reveals strong cytoplasmic staining in a population of small developing neurons. These cells are often found in small clumps as in these cultures, as in this example. Note that they are not positive for MAP2, which is characteristic of more mature neurons, and that the mature neuron shown is negative for NBP1-92684. This Doublecortin antibody is therefore an excellent marker of developing neuronal cells.](http://images.novusbio.com/images2/Doublecortin-Antibody-(3E1)-Immunocytochemistry-Immunofluorescence-NBP1-92684-img0003.jpg)
![Western Blot: RPS19 Antibody (JU33-43) [NBP2-75649] - Analysis of RPS19 on Hela cell using anti-RPS19 antibody at 1/500 dilution.Positive control:Lane 1: CRC Lane 2: MCF-7](http://images.novusbio.com/images2/RPS19 Antibody (JU33-43)-Western Blot-NBP2-75649-img0006.jpg)
![Immunohistochemistry-Paraffin: RPS19 Antibody (JU33-43) [NBP2-75649] - Analysis of paraffin-embedded rat brain tissue using anti-RPS19 antibody. Counter stained with hematoxylin.](http://images.novusbio.com/images2/RPS19-Antibody-(JU33-43)-Immunohistochemistry-Paraffin-NBP2-75649-img0005.jpg)
![Immunohistochemistry-Paraffin: RPS19 Antibody (JU33-43) [NBP2-75649] - Analysis of paraffin-embedded human colon cancer tissue using anti-RPS19 antibody. Counter stained with hematoxylin.](http://images.novusbio.com/images2/RPS19-Antibody-(JU33-43)-Immunohistochemistry-Paraffin-NBP2-75649-img0001.jpg)