CD20 Overexpression Lysate (Native)

Images

 
Western Blot: CD20 Overexpression Lysate (Native) [NBL1-13312] - Detection of CD20 in lymphoma cell line lysates. CD20 over expression lysate was used as the positive control. This image was submitted via customer ...read more
Western Blot: CD20 Overexpression Lysate (Native) [NBL1-13312] - Left-Empty vector transfected control cell lysate (HEK293 cell lysate); Right -Over-expression Lysate for CD20.

Product Details

Summary
Reactivity HuSpecies Glossary
Applications WB

Order Details

CD20 Overexpression Lysate (Native) Summary

Immunogen
The lysate was created in HEK293T cells, using plasmid ID RC221570 and based on accession number NM_152866. The protein contains a C-terminal DDK tag.
Specificity
Homo sapiens membrane-spanning 4-domains, subfamily A, member 1 (MS4A1), transcript variant 1, mRNA.
Gene
MS4A1

Applications/Dilutions

Application Notes
This product is intended for use as a positive control in Western Blot. You will receive the lysate (100ug), and an empty vector negative control (100 ug).
Theoretical MW
32.9 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
Reviewed Applications
Read 1 Review rated 5
using
NBL1-13312 in the following application:

Packaging, Storage & Formulations

Storage
Store at -80C. Avoid freeze-thaw cycles.
Buffer
RIPA buffer

Lysate Details for CD20

Type
Overexpression
Protein State
Native

Notes

HEK293T cells in 10-cm dishes were transiently transfected with a non-lipid polymer transfection reagent specially designed and manufactured for large volume DNA transfection. Transfected cells were cultured for 48hrs before collection. The cells were lysed in modified RIPA buffer (25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4, and then centrifuged to clarify the lysate. Protein concentration was measured by BCA protein assay kit.This product is manufactured by and sold under license from OriGene Technologies and its use is limited solely for research purposes.

Alternate Names for CD20 Overexpression Lysate (Native)

  • B1
  • B-lymphocyte antigen CD20
  • B-lymphocyte cell-surface antigen B1
  • B-lymphocyte surface antigen B1
  • Bp35
  • Bp35MGC3969
  • CD20 antigen
  • CD20 receptor
  • CD20
  • CD20S7
  • CVID5
  • LEU-16
  • Leukocyte surface antigen Leu-16
  • Ly-44
  • Membrane-spanning 4-domains subfamily A member 1
  • membrane-spanning 4-domains, subfamily A, member 1
  • MS4A1
  • MS4A2
  • S7

Background

This gene encodes a member of the membrane-spanning 4A gene family. Members of this nascent protein family are characterized by common structural features and similar intron/exon splice boundaries and display unique expression patterns among hematopoietic cells and nonlymphoid tissues. This gene encodes a B-lymphocyte surface molecule which plays a role in the development and differentiation of B-cells into plasma cells. This family member is localized to 11q12, among a cluster of family members. Alternative splicing of this gene results in two transcript variants which encode the same protein. [provided by RefSeq]. Transcript Variant: This variant (1) represents the longer transcript variant. Variants 1 and 3 both encode the same protein. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Entrez Gene record to access additional publications.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Lysates are guaranteed for 6 months from date of receipt.

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Publications for CD20 Lysate (NBL1-13312) (0)

There are no publications for CD20 Lysate (NBL1-13312).
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Review for CD20 Lysate (NBL1-13312) (1) 51

Average Rating: 5
(Based on 1 review)

Reviews using NBL1-13312:
Filter by Applications
ICC/IF
(1)
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Immunocytochemistry/Immunofluorescence CD20 NBL1-13312
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reviewed by:
Christopher England
ICC/IF 02/24/2017
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Summary

ApplicationImmunocytochemistry/Immunofluorescence

Comments

CommentsLoaded 5 microliter into last well as positive control. In the future, we will load less protein. Overall, it looks great!

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CD20 (Cluster of differentiation 20, Membrane-spanning 4-domains subfamily A member 1 (MS4A1), CVID5, B-lymphocyte surface antigen B1)
CD20 is a human B-lymphocyte surface molecule that spans the membrane four times and is expressed on both normal and malignant cells. The CD20 antigen displays a unique expression pattern among hematopoietic cells - it is present on human pre B-ly...  Read full blog post.

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Recent Reviews

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Christopher England
02/24/2017
Application: ICC/IF
Species:

Bioinformatics

Gene Symbol MS4A1
Entrez