CD20 Antibody (SP32)

Images

 
Western Blot: MS4A1/CD20 Antibody (SP32) [NBP1-49800] - Fluorescent Western blot analysis of CD20 in Daudi whole cell lysate using anti-CD 20 antibody (green). Beta-actin was used as a control antibody (red). Image from ...read more
Immunohistochemistry-Paraffin: MS4A1/CD20 Antibody (SP32) [NBP1-49800] - Human Lung Adenocarcinoma
Flow Cytometry: MS4A1/CD20 Antibody (SP32) [NBP1-49800] - Analysis of rabbit anti-CD20 (SP32) antibody in Jurkat (green) compare to negative control of rabbit IgG (blue)
Western Blot: MS4A1/CD20 Antibody (SP32) [NBP1-49800] - Ramos
Immunohistochemistry-Paraffin: MS4A1/CD20 Antibody (SP32) [NBP1-49800] - Human B Cell Lymphoma.
Immunohistochemistry-Paraffin: MS4A1/CD20 Antibody (SP32) [NBP1-49800] - Human Tonsil
Immunohistochemistry-Paraffin: MS4A1/CD20 Antibody (SP32) [NBP1-49800] - Human Reactive Lymph Node
Immunohistochemistry-Paraffin: MS4A1/CD20 Antibody (SP32) [NBP1-49800] - Human Skin Squamous Cell Carcinoma
Immunohistochemistry-Paraffin: MS4A1/CD20 Antibody (SP32) [NBP1-49800] - Human B Cell Lymphoma
Immunohistochemistry-Paraffin: MS4A1/CD20 Antibody (SP32) [NBP1-49800] - Human HK Lymphoma
Immunohistochemistry-Paraffin: MS4A1/CD20 Antibody (SP32) [NBP1-49800] - Human Colon
Immunohistochemistry-Paraffin: MS4A1/CD20 Antibody (SP32) [NBP1-49800] - Human Spleen
Immunohistochemistry-Paraffin: MS4A1/CD20 Antibody (SP32) [NBP1-49800] - Human Thymus
Immunohistochemistry-Paraffin: MS4A1/CD20 Antibody (SP32) [NBP1-49800] - Human Cervical Squamous Cell Carcinoma

Product Details

Summary
Reactivity HuSpecies Glossary
Applications WB, Flow, IHC, IHC-P
Clone
SP32
Clonality
Monoclonal
Host
Rabbit
Conjugate
Unconjugated

Order Details

CD20 Antibody (SP32) Summary

Immunogen
Synthetic peptides from C-terminus of human CD20 protein
Isotype
IgG
Clonality
Monoclonal
Host
Rabbit
Gene
MS4A1
Purity
Protein A or G purified
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.

Applications/Dilutions

Dilutions
  • Western Blot 1:100-1:2000
  • Flow Cytometry 1:100
  • Immunohistochemistry 1:10-1:500
  • Immunohistochemistry-Paraffin 1:10-1:500
Application Notes
This product is useful for Immunohistochemistry Paraffin. Antigen retrieval can be performed using a Citrate process.
Theoretical MW
35 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
Reviewed Applications
Read 3 Reviews rated 4.3
using
NBP1-49800 in the following applications:

Publications
Read Publication using
NBP1-49800 in the following applications:

  • IHC
    1 publication

Reactivity Notes

Predicted by homology: Canine, Bovine.

Packaging, Storage & Formulations

Storage
Store at 4C. Do not freeze.
Buffer
PBS (pH 7.6) and 1.0% BSA
Preservative
0.1% Sodium Azide
Purity
Protein A or G purified

Notes

For a positive control use Colon and breast.

Alternate Names for CD20 Antibody (SP32)

  • B1
  • B-lymphocyte antigen CD20
  • B-lymphocyte cell-surface antigen B1
  • B-lymphocyte surface antigen B1
  • Bp35
  • Bp35MGC3969
  • CD20 antigen
  • CD20 receptor
  • CD20
  • CD20S7
  • CVID5
  • LEU-16
  • Leukocyte surface antigen Leu-16
  • Ly-44
  • Membrane-spanning 4-domains subfamily A member 1
  • membrane-spanning 4-domains, subfamily A, member 1
  • MS4A1
  • MS4A2
  • S7

Background

CD20 is a B cell-specific 32kDa membrane protein involved in intracellular calcium regulation. CD20 expression begins after the induction of CD19 during development, and is coexpressed with IgM during the pre-B to immature B cell transition in bone marrow. Expression then increases during maturation with almost all mature cells expressing some level of CD20. The human CD20 antigen has proven to be an effective immunotherapeutic target in B cell lymphoma and several autoimmune diseases including rheumatoid arthritis and type I diabetes. CD20 consists of 4 transmembrane domains and a 44 amino acid extracellular loop domain where AISB12 binds.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Publications for CD20 Antibody (NBP1-49800)(1)

We have publications tested in 1 confirmed species: Human.

We have publications tested in 1 application: IHC.


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IHC
(1)
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Filter By Species
Human
(1)
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Reviews for CD20 Antibody (NBP1-49800) (3) 4.33

Average Rating: 4.3
(Based on 3 reviews)
We have 3 reviews tested in 1 species: Human.
We have 3 reviews tested in 2 application: IHC-Fr, WB.

Reviews using NBP1-49800:
Filter by Applications
IHC-Fr
(1)
WB
(2)
All Applications
Filter by Species
Human
(3)
All Species
Images Ratings Applications Species Date Details
Immunohistochemistry-Frozen CD20 NBP1-49800
Enlarge
reviewed by:
Anonymous
IHC-Fr Human 08/27/2017
View

Summary

ApplicationImmunohistochemistry-Frozen
Sample TestedB cell lymphoma
SpeciesHuman
Lot141020LVF
Western Blot CD20 NBP1-49800
Enlarge
reviewed by:
Christopher England
WB Human 12/05/2016
View

Summary

ApplicationWestern Blot
Sample TestedB cell lymphoma
SpeciesHuman
Lot141020LVF

Comments

CommentsWe used a concentration of 1:100 as it is expressed at low levels. The antibody showed some signs of cross-reactivity with possible phosphorylated proteins. Overall, it was a nice antibody.
Western Blot CD20 NBP1-49800
Enlarge
reviewed by:
Christopher England
WB Human 09/04/2016
View

Summary

ApplicationWestern Blot
Sample TestedDaudi whole cell lysate
SpeciesHuman
Lot141020LVF

Blocking

Blocking DetailsLiCor Odyssey PBS Blocker, 1X concentration, 2:00 hr, room temperature

Primary Anitbody

Dilution Ratio1:200, 12 h, 4 degree Celcius, PBS w/0.25% Triton-X

Secondary Antibody

Secondary DescriptionIRDye 800 CW Donkey anti-Rabbit IgG
Secondary Manufacturer Cat#925-32213
Secondary Concentration1:10,000

Details

Detection NotesFluorescent Western blot, LiCor Odyssey, N/A, B-actin acted as the control for normalization

Product General Protocols

Find general support by application which include: protocols, troubleshooting, illustrated assays, videos and webinars.

Video Protocols

WB Video Protocol

FAQs for CD20 Antibody (NBP1-49800) (0)

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Secondary Antibodies

 

Isotype Controls

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Research Areas for CD20 Antibody (NBP1-49800)

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Blogs on CD20.

CD20 (Cluster of differentiation 20, Membrane-spanning 4-domains subfamily A member 1 (MS4A1), CVID5, B-lymphocyte surface antigen B1)
CD20 is a human B-lymphocyte surface molecule that spans the membrane four times and is expressed on both normal and malignant cells. The CD20 antigen displays a unique expression pattern among hematopoietic cells - it is present on human pre B-ly...  Read full blog post.

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Recent Reviews

4.3
5
1
4
2
3
0
2
0
1
0

Anonymous
08/27/2017
Application: IHC-Fr
Species: Human

Christopher England
12/05/2016
Application: WB
Species: Human

Christopher England
09/04/2016
Application: WB
Species: Human

Bioinformatics

Gene Symbol MS4A1
Entrez