CD20 Antibody (IGEL/773) [DyLight 680]

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Product Details

Summary
Reactivity HuSpecies Glossary
Applications WB, Flow, ICC/IF, IHC, IHC-P, ICC/IF, MI
Clone
IGEL/773
Clonality
Monoclonal
Host
Mouse
Conjugate
DyLight 680

Order Details

CD20 Antibody (IGEL/773) [DyLight 680] Summary

Immunogen
Recombinant full-length human CD20 protein
Localization
Predominantly cell surface with some cytoplasmic
Marker
B-Cell Marker
Specificity
Recognizes a protein of 30-33kDa, which is identified as CD20. It is a non-Ig differentiation antigen of B-cells and its expression is restricted to normal and neoplastic B-cells, being absent from all other leukocytes and tissues. CD20 is expressed by pre B-cells and persists during all stages of B-cell maturation but is lost upon terminal differentiation into plasma cells. This MAb can be used for immunophenotyping of leukemia and malignant cells, B lymphocyte detection in peripheral blood and B cell localization in tissues. It reacts with the majority of B-cells present in peripheral blood and lymphoid tissues and their derived lymphomas. In lymphoid tissue, germinal center blasts and B-immunoblasts are particularly reactive. It is a reliable antibody for ascribing a B-cell phenotype in known lymphoid tissues. Rarely, CD20-positive T-cell lymphomas have been reported. Reactivity has also been noted with Reed-Sternberg cells in cases of Hodgkin s disease, particularly of lymphocyte predominant type.
Isotype
IgG2a Kappa
Clonality
Monoclonal
Host
Mouse
Gene
MS4A1
Purity
Protein A or G purified
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.

Applications/Dilutions

Dilutions
  • Flow Cytometry
  • Immunocytochemistry/Immunofluorescence
  • Immunofluorescence
  • Immunohistochemistry
  • Immunohistochemistry-Paraffin
  • Multiplex Immunoassay
  • Western Blot
Application Notes
Use in Multiplex Immunoassay reported in scientific literature (PMID: 31942075).
Optimal dilution of this antibody should be experimentally determined.

Packaging, Storage & Formulations

Storage
Store at 4C in the dark.
Buffer
50mM Sodium Borate
Preservative
0.05% Sodium Azide
Purity
Protein A or G purified

Notes

Dylight (R) is a trademark of Thermo Fisher Scientific Inc. and its subsidiaries. This conjugate is made on demand. Actual recovery may vary from the stated volume of this product. The volume will be greater than or equal to the unit size stated on the datasheet.

Alternate Names for CD20 Antibody (IGEL/773) [DyLight 680]

  • B1
  • B-lymphocyte antigen CD20
  • B-lymphocyte cell-surface antigen B1
  • B-lymphocyte surface antigen B1
  • Bp35
  • Bp35MGC3969
  • CD20 antigen
  • CD20 receptor
  • CD20
  • CD20S7
  • CVID5
  • LEU-16
  • Leukocyte surface antigen Leu-16
  • Ly-44
  • Membrane-spanning 4-domains subfamily A member 1
  • membrane-spanning 4-domains, subfamily A, member 1
  • MS4A1
  • MS4A2
  • S7

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Product General Protocols

Find general support by application which include: protocols, troubleshooting, illustrated assays, videos and webinars.

Video Protocols

WB Video Protocol
ICC/IF Video Protocol

FAQs for CD20 Antibody (NBP2-47840FR) (0)

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Secondary Antibodies

 

Isotype Controls

Other Available Formats

Alexa Fluor 350 NBP2-47840AF350
Alexa Fluor 405 NBP2-47840AF405
Alexa Fluor 488 NBP2-47840AF488
Alexa Fluor 532 NBP2-47840AF532
Alexa Fluor 594 NBP2-47840AF594
Alexa Fluor 647 NBP2-47840AF647
Alexa Fluor 700 NBP2-47840AF700
Alexa Fluor 750 NBP2-47840AF750
Allophycocyanin NBP2-47840APC
Allophycocyanin/Cy7 NBP2-47840APCCY7
Biotin NBP2-47840B
DyLight 350 NBP2-47840UV
DyLight 405 NBP2-47840V
DyLight 488 NBP2-47840G
DyLight 550 NBP2-47840R
DyLight 594 NBP2-47840DL594
DyLight 650 NBP2-47840C
DyLight 680 NBP2-47840FR
DyLight 755 NBP2-47840IR
FITC NBP2-47840F
HRP NBP2-47840H
Janelia Fluor 549 NBP2-47840JF549
Janelia Fluor 646 NBP2-47840JF646
PE NBP2-47840PE
PE/Atto594 NBP2-47840PEATT594
PE/Cy5.5 NBP2-47840PECY55
PE/Cy7 NBP2-47840PECY7
PerCP NBP2-47840PCP

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Antigen-loss relapse after successful CAR-T therapy; What do we do now?
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Bioinformatics

Gene Symbol MS4A1