CD20 Antibody (E17-P)

Images

 
Immunohistochemistry-Paraffin: MS4A1/CD20 Antibody (E17-P) [NBP1-30144] - CD20 expression in b-lymphocytes of the palatine tonsil. Formalin fixed, paraffin embedded human tissues (4 um sections) stained with anti- CD20 ...read more
Immunohistochemistry-Paraffin: MS4A1/CD20 Antibody (E17-P) [NBP1-30144] - Chronic lymphocytic leukaemia cells in the bone marrow biopsy, showing CD20 expression.Formalin fixed, paraffin embedded human tissues (4 um ...read more

Product Details

Summary
Reactivity HuSpecies Glossary
Applications IHC, IHC-P
Clone
E17-P
Clonality
Monoclonal
Host
Rabbit
Conjugate
Unconjugated

Order Details

CD20 Antibody (E17-P) Summary

Additional Information
Recombinant Monoclonal Antibody.
Immunogen
Peptide derived from C-terminal sequence of human CD20. Antibody recognizes the epitope between Pro283 - Ser295.
Specificity
This antibody is specific for the C-terminus of CD20.
Isotype
IgG
Clonality
Monoclonal
Host
Rabbit
Gene
MS4A1
Purity
Protein A or G purified
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.

Applications/Dilutions

Dilutions
  • Immunohistochemistry 1:100 - 1:200
  • Immunohistochemistry-Paraffin 1:100 - 1:200
Application Notes
Paraffin embedded sections. For 0.1ml, 0.5ml and 1.0ml sizes, use recommended dilutions. For 7.0ml and 15.0ml sizes, products should be used undiluted.
Publications
Read Publication using NBP1-30144.

Packaging, Storage & Formulations

Storage
Store at 4C. Do not freeze.
Buffer
20mM Tris/HCl (pH 8.0) and 20 mg/ml BSA
Preservative
0.05% Sodium Azide
Purity
Protein A or G purified

Notes

The 7mL and 15 mL sizes that this product is available in is a pre-diluted size and no additional dilutions are required before using this item for the intended application.

Alternate Names for CD20 Antibody (E17-P)

  • B1
  • B-lymphocyte antigen CD20
  • B-lymphocyte cell-surface antigen B1
  • B-lymphocyte surface antigen B1
  • Bp35
  • Bp35MGC3969
  • CD20 antigen
  • CD20 receptor
  • CD20
  • CD20S7
  • CVID5
  • LEU-16
  • Leukocyte surface antigen Leu-16
  • Ly-44
  • Membrane-spanning 4-domains subfamily A member 1
  • membrane-spanning 4-domains, subfamily A, member 1
  • MS4A1
  • MS4A2
  • S7

Background

CD20 is a cell surface 33-37 (depending on the degree of phosphorylation) kDa non-glycosylated surface phosphoprotein expressed on mature and most malignant B cells, but not stem cells or plasma cells (low number of the CD20 has been also detected on a subpopulation of T lymphocytes and it can be expressed on follicular dendritic cells). Its expression on B cells is synchronous with the expression of surface IgM. CD20 regulates transmembrane calcium conductance (probably functioning as a component of store-operated calcium channel), cell cycle progression and B-cell proliferation. It is associated with lipid rafts, but the intensity of this association depends on extracellular triggering, employing CD20 conformational change and/or BCR (B cell antigen receptor) aggregation. After the receptor ligation, BCR and CD20 colocalize and then rapidly dissociate before BCR endocytosis, whereas CD20 remains at the cell surface. CD20 serves as a useful target for antibody-mediated therapeutic depletion of B cells, as it is expressed at high levels on most B-cell malignancies, but does not become internalized or shed from the plasma membrane following mAb treatment.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Publications for CD20 Antibody (NBP1-30144)(1)

We have publications tested in 1 application: IHC-P.


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IHC-P
(1)
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Product General Protocols

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Secondary Antibodies

 

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Antigen-loss relapse after successful CAR-T therapy; What do we do now?
By Jacqueline Carrico, BS, MD Tumor cell mechanisms driving tolerance to CAR-T Despite very promising results of CAR-T therapy in acute lymphoblastic leukemia, B-ALL, antigen-loss relapse has arisen as a major chall...  Read full blog post.

CD20 (Cluster of differentiation 20, Membrane-spanning 4-domains subfamily A member 1 (MS4A1), CVID5, B-lymphocyte surface antigen B1)
CD20 is a human B-lymphocyte surface molecule that spans the membrane four times and is expressed on both normal and malignant cells. The CD20 antigen displays a unique expression pattern among hematopoietic cells - it is present on human pre B-ly...  Read full blog post.

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Bioinformatics

Gene Symbol MS4A1
Entrez
Uniprot