CD2 Antibody (12-15)

Images

 
Flow Cytometry: CD2 Antibody (12-15) [NBP1-26666] - BALB/c mouse splenocytes were stained with Rat Anti-Mouse CD2-FITC (NBP1-26667).
Flow Cytometry: CD2 Antibody (12-15) [NBP1-26666] - Analysis using the Biotin conjugate of NBP1-26666. Multiple staining of C57BL/6 splenocytes.
Flow Cytometry: CD2 Antibody (12-15) [NBP1-26666] - Analysis using the FITC conjugate of NBP1-26666. Staining of 1 ug/1^6 BALB/c lymph node cells with rat anti-mouse CD2-FITC. Lymphocytes were then gated and analyzed by ...read more
Flow Cytometry: CD2 Antibody (12-15) [NBP1-26666] - Analysis using the FITC conjugate of NBP1-26666. Staining of C57BL/6 mouse splenocytes with NBP1-26669 (PE conjugate) and NBP1-26667 (FITC conjugate).
Flow Cytometry: CD2 Antibody (12-15) [NBP1-26666] - Analysis using the PE conjugate of NBP1-26666. Staining of C57BL/6 mouse splenocytes with NBP1-26669 (PE conjugate) and NBP1-26667 (FITC conjugate).

Product Details

Summary
Reactivity MuSpecies Glossary
Applications B/N, Flow, In vitro, IP
Clone
12-15
Clonality
Monoclonal
Host
Rat
Conjugate
Unconjugated
Concentration
0.5 mg/ml

Order Details

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Catalog# & Conjugate Size Price

CD2 Antibody (12-15) Summary

Immunogen
Crude plasma membranes of ESb cells
Specificity
Mouse CD2/LFA-2 (Mr 45-58 kDa)
Isotype
IgG1 Kappa
Clonality
Monoclonal
Host
Rat
Gene
CD2
Purity
Protein A or G purified
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.

Applications/Dilutions

Dilutions
  • Blocking/Neutralizing 50-200 molar excess
  • Flow Cytometry 1:10 - 1:1000
  • Immunoprecipitation 1:10 - 1:500
  • In vitro assay
Application Notes
Flow Cytometry, Immunoprecipitation, In vitro inhibition of CTL-mediated lysis, Induction of T cell unresponsiveness in vivo
Publications
Read Publication using
NBP1-26666 in the following applications:

Packaging, Storage & Formulations

Storage
Store at 4C. Do not freeze.
Buffer
0.1M BBS (pH 8.2)
Preservative
No Preservative
Concentration
0.5 mg/ml
Purity
Protein A or G purified

Alternate Names for CD2 Antibody (12-15)

  • CD2 antigen (p50), sheep red blood cell receptor
  • CD2 antigen
  • CD2 molecule
  • CD2
  • Erythrocyte receptor
  • FLJ46032
  • Leu-5
  • LFA-2
  • LFA-3 receptor
  • Ly37
  • lymphocyte-function antigen-2
  • Rosette receptor
  • SRBC
  • SRBC-R
  • T11
  • T-cell surface antigen CD2
  • T-cell surface antigen T11/Leu-5

Background

CD2, also known as leukocyte function-associated antigen-2 (LFA-2), is a member of the immunoglobulin superfamily of cell adhesion molecules. It is expressed on the surface of >95% of thymocytes, and essentially all B cells, T cells and NK cells. In the mouse, CD2 is the receptor for CD48. CD2 is thought to play a major role in mediating T-cell adhesion, T-cell recognition/activation, T-cell signaling, and intrathymic differentiation.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Publications for CD2 Antibody (NBP1-26666)(1)

We have publications tested in 1 confirmed species: Mouse.

We have publications tested in 1 application: FLOW.


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FLOW
(1)
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Mouse
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Product General Protocols

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FAQs for CD2 Antibody (NBP1-26666). (Showing 1 - 1 of 1 FAQ).

  1. Does this antibody cross react with the FC receptor?
    • The answer to your question is likely to be "NO", this antibody should not be cross reactive with the mouse FC receptors, based on the following reasons: 1) Since we do not map the epitopes of our antibodies and the immunogen sequence of this antibody is the proprietary information, I think it is safe to say that the extracellular domain (a.a. 32 - 302 on Uniprot) of the mouse CD2 protein contains the epitope, where NBP1-26666 interacts with. Based on this assumption, I have done an a.a. sequence comparison. 2) There are several different types of Fc receptors (FcR) and the major ones are those binding to IgG, which are called Fc-gamma receptors (Fc gamma R). Fc gamma R belongs to the immunoglobulin superfamily and includes several members, Fc gamma RI (CD64), Fc gamma RIIA (CD32), Fc gamma RIIB (CD32), etc., which differ in their antibody affinities due to their different molecular structure. Therefore the a.a. sequences derived from mouse CD64 and CD32 were compared with the sequence of mouse CD2 at the a.a. 32 - 302.  

Secondary Antibodies

 

Isotype Controls

Other Available Formats

Biotin Labeled NBP1-26668
FITC Labeled NBP1-26667
PE Labeled NBP1-26669

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Bioinformatics

Gene Symbol CD2
Entrez