CD1d Antibody (703335) [Alexa Fluor® 750]

• Applications: WB
• Clone: 703335
• Clonality: Monoclonal
• Host: Mouse
• Conjugate: Alexa Fluor 750

CD1d Antibody (703335) [Alexa Fluor® 750] Summary

• Immunogen: Mouse myeloma cell line NS0-derived recombinant human CD1d
  Glu20-Ser301 (predicted)
  Accession # P15813
• Specificity: Detects human CD1d in direct ELISAs. In direct ELISAs, no cross-reactivity with recombinant human (rh) CD1a, rhCD1b, rhCD1c, rhCD1e, recombinant mouse (rm) CD1d1, and rmCD1d2 is observed.
• Isotype: IgG2b
• Clonality: Monoclonal
• Host: Mouse
• Purity Statement: Protein A or G purified

Packaging, Storage & Formulations

• Storage: Protect from light. Do not freeze. 12 months from date of receipt, 2 to 8 °C as supplied
• Buffer: Supplied 0.2mg/ml in 1X PBS with RDF1 and 0.09% Sodium Azide

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Background

CD1d is a 48 kDa transmembrane glycoprotein in the CD1 family of glycolipid antigen-presenting MHC-like molecules. Mature human CD1d consists of a 282 amino acid (aa) extracellular domain (ECD) with one Ig-like domain, a 21 aa transmembrane segment, and a 13 aa cytoplasmic tail. Within aa 19-300 (the ECD), human CD1d shares 65% and 68% aa sequence identity with mouse and rat CD1d, respectively. Complexes of CD1d with beta 2-microglobulin and endogenous glycolipids are constitutively expressed on antigen presenting cells, cortical thymocytes, liver sinusoidal endothelial cells, Kupffer cells, and hepatocytes (1). CD1d-presented glycolipids are recognized by canonical NKT cells that utilize an invariant mouse Va14-Ja18 chain in their T cell receptor (Va24-Ja18 in human) (2, 3). The interaction with glycolipid-loaded CD1d is critical for NKT cell development and induces their rapid secretion of both Th1 and Th2 type cytokines (3-6). In humans, infection with HSV-1 suppresses NKT cell activation by blocking the intracellular cycling of CD1d in antigen presenting cells (7).

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

FAQs for CD1d Antibody (FAB6979S). (Showing 1 - 1 of 1 FAQ).

1. Do you have an antibody for CD1d that works well on formalin fixed paraffin embedded tissue (specifically for liver tissue)?
   • We do have a CD1d antibody that has been verified in paraffin-embedded tissue sections. The catalog number is NBP1-43461.