CD1d Antibody (51.1)

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Flow Cytometry: CD1d Antibody (51.1) [NBP1-43460] - Staining of normal human peripheral blood cells with 0.5 ug of Mouse IgG2b K Isotype Control Purified (open histogram) or Anti-Human CD1d Purified (filled histogram) ...read more

Product Details

Summary
Reactivity HuSpecies Glossary
Applications Flow, IHC, IHC-Fr, IP
Clone
51.1
Clonality
Monoclonal
Host
Mouse
Conjugate
Unconjugated
Concentration
0.5 mg/ml

CD1d Antibody (51.1) Summary

Immunogen
The immunogen for this antibody was CD1d.
Isotype
IgG2b
Clonality
Monoclonal
Host
Mouse
Gene
CD1D
Purity
Protein A or G purified
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.

Applications/Dilutions

Dilutions
  • Flow Cytometry 1:10-1:1000
  • Immunohistochemistry 1:10-1:500
  • Immunohistochemistry-Frozen 1:10-1:500
  • Immunoprecipitation 1:10-1:500
Application Notes
This 51.1 antibody has been tested by flow cytometric analysis of normal human peripheral blood cells. This can be used at less than or equal to 0.5 ug per test. Cell number should be determined empirically but can range from 10^5to 10^8cells/test.

Packaging, Storage & Formulations

Storage
Store at 4C. Do not freeze.
Buffer
PBS, pH 7.2
Preservative
0.09% Sodium Azide
Concentration
0.5 mg/ml
Purity
Protein A or G purified

Alternate Names for CD1d Antibody (51.1)

  • antigen-presenting glycoprotein CD1d
  • CD1A
  • CD1d antigen
  • CD1D antigen, d polypeptide
  • CD1d molecule
  • CD1d
  • differentiation antigen CD1-alpha-3
  • HMC class I antigen-like glycoprotein CD1D
  • MGC34622
  • R3
  • R3G1
  • T-cell surface glycoprotein CD1d
  • thymocyte antigen CD1D

Background

The monoclonal antibody 51.1 reacts with human CD1d, a member of the CD1 family with similarity to the non-polymorphic MHC Class I-like molecules. CD1d is a highly conserved single transmembrane receptor of the Immunoglobulin Superfamily. CD1d can associate with beta-microglobulin another feature showing similarity to MHC class I molecules, but can also exist as a nonglycosylated protein not in association with beta microglobulin. This suggests different control mechanisms for presenting glycolipid containing molecules to CD1d reactive NKT cells. Expression of CD1d is found on B cells of the periphery, in resting monocytes and cortical thymocytes. On intestinal epithelial cells (IEC) expression is polarized. Expression can also be found at low levels intracellularly in hepatocytes. In HCV (hepatitis C virus) livers, CD1d is highly expressed compared to normal controls.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Product General Protocols

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FAQs for CD1d Antibody (NBP1-43460). (Showing 1 - 1 of 1 FAQ).

  1. Do you have an antibody for CD1d that works well on formalin fixed paraffin embedded tissue (specifically for liver tissue)?
    • We do have a CD1d antibody that has been verified in paraffin-embedded tissue sections. The catalog number is NBP1-43461.

Secondary Antibodies

 

Isotype Controls

Other Available Formats

Alexa Fluor 405 Labeled NBP1-43460AF405
Alexa Fluor 488 Labeled NBP1-43460AF488
Alexa Fluor 647 Labeled NBP1-43460AF647
Alexa Fluor 700 Labeled NBP1-43460AF700
Allophycocyanin Labeled NBP1-43460APC
DyLight 350 Labeled NBP1-43460UV
DyLight 405 Labeled NBP1-43460V
DyLight 488 Labeled NBP1-43460G
DyLight 680 Labeled NBP1-43460FR
DyLight 755 Labeled NBP1-43460IR
PE Labeled NBP1-43460PE
PerCP Labeled NBP1-43460PCP

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Bioinformatics

Gene Symbol CD1D
Entrez