CD1c/BDCA-1 Antibody [Alexa Fluor® 405]

• Reactivity: Hu
• Applications: WB
• Clonality: Polyclonal
• Host: Goat
• Conjugate: Alexa Fluor 405

CD1c/BDCA-1 Antibody [Alexa Fluor® 405] Summary

• Immunogen: Mouse myeloma cell line NS0-derived recombinant human CD1c/BDCA‑1
  Ala19-Met302 (Phe300Ser)
  Accession # P29017
• Specificity: Detects human CD1c in direct ELISAs and Western blots. In direct ELISAs, less than 1% cross‑reactivity with recombinant human (rh) CD1a, rhCD1b, rhCD1d, and rhCD1e is observed.
• Isotype: IgG
• Clonality: Polyclonal
• Host: Goat
• Purity Statement: Antigen Affinity-purified

Applications/Dilutions

• Dilutions:
  • Western Blot

Packaging, Storage & Formulations

• Storage: Protect from light. Do not freeze. 12 months from date of receipt, 2 to 8 °C as supplied
• Buffer: Supplied 0.2mg/ml in 1X PBS with RDF1 and 0.09% Sodium Azide

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for CD1c/BDCA-1 Antibody [Alexa Fluor® 405]

• BDCA1
• BDCA-1
• c polypeptide
• CD1
• CD1A
• CD1c antigen
• CD1c molecule
• CD1c
• cortical thymocyte antigen CD1C
• differentiation antigen CD1-alpha-3
• R7
• T-cell surface glycoprotein CD1c

Background

CD1c (Cluster of differentiation antigen 1c) is a 316 amino acid glycoprotein with a predicted molecular weight of 43 kDa. CD1c is a member of the CD1 family of molecules. It is expressed by thymocytes, dendritic cells and B cells, and exists as part of a noncovalent complex with 12 kDa beta ₂‑microglobulin. It is found in the plasma membrane and early endosomes (but not lysosomes), and is presumed to present glycolipids and acylated peptides to T cells. Mature human CD1c is a 316 amino acid (aa) type I transmembrane glycoprotein. It contains a 285 aa extracellular domain (ECD) (aa 18‑302) plus a 10 aa cytoplasmic tail. The ECD shows one Ig‑like domain (aa 203‑296) that associates with beta ₂‑microglobulin, and a TyrGlnAspIle internalization motif in the cytoplasmic tail. There are three potential splice variants. One shows a Trp substitution for aa 327‑333, a second shows an eight aa substitution for aa 298‑333, and a third shows a 50 aa substitution for aa 297‑333. There appears to be no direct mouse counterpart to human CD1c.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.