CD1b Antibody (100-1A5) - Azide and BSA Free

Product Discontinued

CD1b Antibody (100-1A5) - Azide and BSA Free Summary

• Description: Purified from Bioreactor Concentrate by Protein A or G. Prepared in 10 mM PBS WITHOUT BSA & azide at 1.0 mg/ml.
• Additional Information: Clone 100-1A5 was used by HLDA to establish CD designation.
• Immunogen: Stimulated human leukocytes
• Localization: Cell surface
• Specificity: The mouse monoclonal antibody recognizes CD1b, a 44kDa type I glycoprotein associated with beta2-microglobulin (Workshop IV; Code T015). It is expressed on dendritic cells, Langerhans cells, thymocytes, and T acute lymphoblastic leukemia cells. The CD1 multigene family encodes five forms of the CD1 T-cell surface glycoprotein in human, designated CD1A, 1B, 1C, 1D and 1E. CD1, a type 1 membrane protein, has structural similarity to the MHC class I antigen and has been shown to present lipid antigens for recognition by T lymphocytes. Constitutive endocytosis of CD1B molecules and the differential sorting of MHC class II from lysosomes separate peptide- and lipid antigen-presenting molecules during dendritic cell maturation. CD1B is also expressed in interdigitating cells.
• Isotype: IgM Kappa
• Clonality: Monoclonal
• Host: Mouse
• Gene: CD1B
• Purity: Protein L purified

Applications/Dilutions

• Dilutions:
  • CyTOF-ready
  • Flow Cytometry 0.5 - 1 ug/million cells in 0.1 ml
  • Immunocytochemistry/ Immunofluorescence 1-2 ug/ml
  • Immunofluorescence 0.5 - 1.0 ug/ml
• Application Notes: Optimal dilution for a specific application should be determined.
• Theoretical MW: 44 kDa.
  Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Packaging, Storage & Formulations

• Storage: Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
• Buffer: 10 mM PBS with 0.05% BSA
• Preservative: No Preservative
• Concentration: 0.2 mg/ml
• Purity: Protein L purified

Alternate Names for CD1b Antibody (100-1A5) - Azide and BSA Free

• CD1
• CD1A
• CD1b antigen
• CD1B antigen, b polypeptide
• CD1b molecule
• CD1b
• cortical thymocyte antigen CD1B
• differentiation antigen CD1-alpha-3
• MGC125990
• MGC125991
• R1
• T-cell surface glycoprotein CD1b

Background

All CD1 molecules, except CD1e, are cell surface glycoproteins that are structurally related to the MHC molecules, however, in distinction, CD1 proteins are essentially non polymorphic. CD1 has considerable structural homology with both MHC class I and class II molecules, and CD1 molecules are involved in T cell activation. In contrast to MHC, however, CD1 molecules appear to present predominantly non peptide molecules originating from lipids and glycolipids.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Bioinformatics

• Gene Symbol: CD1B
• Uniprot:
  • P29016()