CD161/NK1.1 Antibody (PK-136) - Chimeric - Azide and BSA Free

Immunocytochemistry /Immunofluorescence: CD161/NK1.1 Antibody (PK-136) - Chimeric - Azide and BSA Free [NBP3-20165] - Immunofluorescence analysis of paraformaldehyde fixed mouse splenocytes on Shi-fix coverslips stained with NBP3-20165 at 10 ug/ml for 1h followed by Alexa Fluor 488 secondary antibody (2 ug/ml), showing membrane staining. The nuclear stain is DAPI (blue). Panels show from left-right, top-bottom NBP3-20165, DAPI, merged channels and an isotype control. The isotype control was an unknown specificity antibody followed by staining with Alexa Fluor 488 secondary antibody.

Flow Cytometry: CD161/NK1.1 Antibody (PK-136) - Chimeric - Azide and BSA Free [NBP3-20165] - Paraformaldehyde fixed mouse splenocytes permeabilized with 0.5% Triton were stained with anti-unknown specificity antibody (isotype control, black line) or NBP3-20165 (blue line) at a dilution of 1:100 for 1h at RT. After washing, the bound antibody was detected using a goat anti-rabbit IgG AlexaFluor 488 antibody at a dilution of 1:1000 and cells analyzed using a FACSCanto flow-cytometer.

Western Blot: CD161/NK1.1 Antibody (PK-136) - Chimeric - Azide and BSA Free [NBP3-20165] - RAW264.7(A) (1ug/ml) cell lysate, mouse spleen(B) (1ug/ml) and mouse lymph node(C) (0.3ug/ml) tissue lysate (35ug protein in RIPA buffer) were resolved on a SDS PAGE gel and blots were probed with NBP3-20165 before detection using an anti-rabbit secondary antibody. A primary incubation of 1h was used and protein was detected by chemiluminescence.

• Reactivity: Mu
• Applications: ELISA, Flow, Cell Depl
• Clone: PK-136
• Clonality: Monoclonal
• Host: Rabbit
• Conjugate: Unconjugated
• Format: Azide and BSA Free
• Concentration: 1 mg/ml

CD161/NK1.1 Antibody (PK-136) - Chimeric - Azide and BSA Free Summary

• Description: Novus Biologicals Rabbit CD161/NK1.1 Antibody (PK-136) - Chimeric - Azide and BSA Free (NBP3-20165) is a recombinant monoclonal antibody validated for use in ELISA and Flow. All Novus Biologicals antibodies are covered by our 100% guarantee.
• Additional Information: Recombinant Monoclonal Antibody
• Immunogen: The original antibody was generated by immunizing (C3H X BALB)F1 mice with spleen cells enriched for NK-1 cells and bone marrow cells from CE mice.
• Isotype: IgG Kappa
• Clonality: Monoclonal
• Host: Rabbit
• Gene: Klrb1c
• Purity: Protein A purified

Applications/Dilutions

• Dilutions:
  • Cell depletion
  • ELISA
  • Flow Cytometry
• Application Notes: This chimeric rabbit antibody was made using the variable domain sequences of the original Mouse IgG2a format for improved compatibility with existing reagents assays and techniques.

Packaging, Storage & Formulations

• Storage: Store at 4C for up to 3 months. For longer storage, aliquot and store at -20C.
• Buffer: PBS
• Preservative: 0.02% Proclin 300
• Concentration: 1 mg/ml
• Purity: Protein A purified

Alternate Names for CD161/NK1.1 Antibody (PK-136) - Chimeric - Azide and BSA Free

• CD161 antigen
• CD161
• CD161/NK1.1
• CD161c
• CLEC5BC-type lectin domain family 5 member B
• HNKR-P1a
• killer cell lectin-like receptor subfamily B member 1
• killer cell lectin-like receptor subfamily B, member 1
• Klrb1c
• Ly55c
• Ly-59
• Natural killer cell surface protein P1A
• NKR-P1
• NKR-P1AMGC138614
• NKRP1ANKR

Background

NK-1.1 surface antigen is encoded by the NKR-P1B/NKR-P1C gene, also known as CD161b/CD161c and Ly-55. It is expressed on NK cells and NK-T cells in some mouse strains, including C57BL/6, FVB/N, and NZB, but not AKR, BALB/c, CBA/J, C3H, DBA/1, DBA/2, NOD, SJL, and 129. Expression of NKR-P1C antigen has been correlated with lysis of tumor cells in vitro and rejection of bone marrow allografts in vivo. NK-1.1 has also been shown to play a role in NK cell activation, IFN- gamma production, and cytotoxic granule release. NK-1.1 and DX5 are commonly used as mouse NK cell markers. The PK136 antibody has been reported to deplete NK cells, induce redirected lysis, block NK cell function, and induce NK cell proliferation.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Bioinformatics

• Gene Symbol: Klrb1c