Recombinant Mouse CD14 Protein

Product Discontinued

Recombinant Mouse CD14 Protein Summary

• Description: Recombinant bioactive protein for Mouse CD14
  
  

  Source:CHO cells
  Amino Acid Sequence:(P10810)

• Details of Functionality: Inhibits binding of LPS to CD14. Up to 5ug/ml mouse CD14 inhibit binding of FITC-LPS (0.5ug/ml) to mouse CD14+ CHO transfectants
• Source: CHO
• Protein/Peptide Type: Recombinant Protein
• Gene: CD14
• Purity: >95%, by SDS-PAGE
• Endotoxin Note: <0.1 ng/ug

Applications/Dilutions

• Dilutions:
  • Bioactivity
  • SDS-Page
• Theoretical MW: 50 kDa.
  Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Packaging, Storage & Formulations

• Storage: Store at -20 to -80C. Avoid freeze-thaw cycles.
• Buffer: Lyophilized from PBS (pH 7.2).
• Concentration: Lyoph
• Purity: >95%, by SDS-PAGE
• Reconstitution Instructions: Reconstitute in 10 ul of ultra-pure distilled water. This solution can then be diluted into other aqueous buffers and stored at -20C or -70C for long-term storage.

Alternate Names for Recombinant Mouse CD14 Protein

• CD14 antigen
• CD14 molecule
• CD14
• monocyte differentiation antigen CD14
• Myeloid cell-specific leucine-rich glycoprotein

Background

CD14 acts as a receptor for endotoxin (LPS), is expressed strongly on monocytes, macrophages and weekly at surface of neutrophiles. CD14 is anchored to cells by linkage to glycosylphosphatidylinositol (GPI) and functions as a high affinity receptor LPS-LBP (lipopolysaccharide binding protein)-complexes. Furthermore CD14 is present in a soluble form in human serum, urine and other body fluids, acts at physiological concentrations as LPS agonist and has at higher concentrations an antagonizing effect in cell activation. The myeloid differentiation antigen CD14 acts as the major receptor for bacterial LPS. The dominant form of the recombinant wild type CD14 is the 50 kDa protein. This is a His-tagged protein.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Peptides and proteins are guaranteed for 3 months from date of receipt.

Bioinformatics

• Gene Symbol: CD14