CD14 Antibody (Tuk4)

Images

 
Flow Cytometry: CD14 Antibody (Tuk4) [NBP2-22153] - CD14 in Canine whole blood cells immunophenotyping.-image courtesy of customer.
Flow Cytometry: CD14 Antibody (Tuk4) [NBP2-22153] - Analysis using the FITC conjugate of NBP2-22153. Staining of human peripheral blood monocytes probed.
Flow Cytometry: CD14 Antibody (Tuk4) [NBP2-22153] - Analysis using the PE conjugate of NBP2-22153. Staining of human peripheral blood monocytes probed with MOUSE ANTI HUMAN CD14:RPE.

Product Details

Summary
Reactivity Hu, Po, Bv, Ca, Fe, Gt, Ll, Md, Pm, Rb, ShSpecies Glossary
Applications WB, ELISA, Flow
Clone
Tuk4
Clonality
Monoclonal
Host
Mouse
Conjugate
Unconjugated
Concentration
1 mg/ml

CD14 Antibody (Tuk4) Summary

Immunogen
Made to Human CD14
Localization
Cell Membrane
Specificity
This antinody recognises the human CD14 cell surface antigen, expressed strongly by most peripheral blood monocytes and weakly on granulocytes. Clone Tuk4 has been shown to block SDF-induced chemotaxis of U937 cells in a dose-dependent manner, see Yang, H. et al. for details.
Isotype
IgG2a
Clonality
Monoclonal
Host
Mouse
Gene
CD14
Purity
Protein A or G purified
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.

Applications/Dilutions

Dilutions
  • Western Blot 1:100-1:2000
  • ELISA 1:100-1:2000
  • Flow Cytometry 1:50-1:200
Positive Control
AP2S1 Recombinant Protein (H00001175-P01)
Reviewed Applications
Read 1 Review rated 5
using
NBP2-22153 in the following applications:

Packaging, Storage & Formulations

Storage
Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
Buffer
PBS
Preservative
0.09% Sodium Azide
Concentration
1 mg/ml
Purity
Protein A or G purified

Alternate Names for CD14 Antibody (Tuk4)

  • CD14 antigen
  • CD14 molecule
  • CD14
  • monocyte differentiation antigen CD14
  • Myeloid cell-specific leucine-rich glycoprotein

Background

CD14 is a single copy gene encoding 2 protein forms: a 50 to 55 kDa glycosylphosphatidylinositol anchored membrane protein (mCD14) and a monocyte or liver derived soluble serum protein (sCD14) that lacks the anchor. Both molecules are critical for lipopolysaccharide (LPS) dependent signal transduction, and sCD14 confers LPS sensitivity to cells lacking mCD14. Increased sCD14 levels are associated with inflammatory infectious diseases and high mortality in gram negative shock. CD14 also appears to be involved in clearance of gram-negative bacteria via its high affinity binding to LPS-LPB complexes.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Publications for CD14 Antibody (NBP2-22153) (0)

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Review for CD14 Antibody (NBP2-22153) (1) 51

Average Rating: 5
(Based on 1 review)
We have 1 review tested in 1 species: Canine.
We have 1 review tested in 1 application: Flow.

Reviews using NBP2-22153:
Filter by Applications
Flow
(1)
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Filter by Species
Canine
(1)
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Images Ratings Applications Species Date Details
Flow Cytometry CD14 NBP2-22153
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reviewed by:
A G
Flow Canine 02/27/2017
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Summary

ApplicationFlow Cytometry
Sample TestedWhole blood cells
SpeciesCanine
Lot1568

Product General Protocols

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Video Protocols

WB Video Protocol

FAQs for CD14 Antibody (NBP2-22153) (0)

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Positive Control Lysate(s)

Secondary Antibodies

 

Isotype Controls

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Research Areas for CD14 Antibody (NBP2-22153)

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Blogs on CD14.

The role of TLR4 in breast cancer
Toll like receptors (TLRs) are highly conserved proteins that are first known for their role in pathogen recognition and immune response activation.  In order to elicit the necessary immune response in reaction to a foreign pathogen, TLRs trigger cy...  Read full blog post.

CD14 - TLR4 is my friend in battle against infections!
CD14 is a well-characterized cell-activating receptor for lipopolysaccharide-binding protein (LBP) and peptidoglycan. It is an important modulator for lipopolysaccharide (LPS)-dependent signaling and is a component of the multi-protein complex contain...  Read full blog post.

CD11b: Marker for a New Type of B Cell that Participates in Cell-Mediated Immunity
Think B lymphocytes just produce antibodies? Think again! Although, of course, B cells are vital for the humoral immune response, many studies in recent years have begun to uncover antibody-independent actions of B cells: regulating T cells and thus a...  Read full blog post.

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Recent Reviews

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1
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A G
02/27/2017
Application: Flow
Species: Canine

Bioinformatics

Gene Symbol CD14
Entrez