CD14 Antibody (TUK4) [HRP]

• Reactivity: Hu
• Applications: ELISA, Flow
• Clone: TUK4
• Clonality: Monoclonal
• Host: Mouse
• Conjugate: HRP

CD14 Antibody (TUK4) [HRP] Summary

• Description: This conjugate is made on demand and is not held in inventory. Each order represents a unique lot. All conjugates are generated using validated conjugation protocols and meet strict degree of labeling (F/P) specifications; additional information is available upon request. The antibody concentration is provided on the individual product label.
  
  For specialized conjugation needs, like large quantities, specific concentrations, and defined F/P ratios, bulk and custom antibody conjugation services are available.
• Additional Information: Clone TUK4 was used by HLDA to establish CD designation.
• Immunogen: Made to Human CD14
• Localization: Cell Membrane
• Specificity: This product recognises the human CD14 cell surface antigen, expressed strongly by most peripheral blood monocytes and weakly on granulocytes. Clone Tuk4 has been shown to block SDF-induced chemotaxis of U937 cells in a dose-dependent manner(6).
• Isotype: IgG2a
• Clonality: Monoclonal
• Host: Mouse
• Gene: CD14
• Purity: Protein G purified

Applications/Dilutions

• Dilutions:
  • ELISA
  • Flow Cytometry
• Application Notes: Recommended applications are based on validated applications from the unconjugated base product NB100-62994. This conjugated antibody is not kept in inventory and is made to order using validated conjugation protocols.

Reactivity Notes

Predicted cross-reactivities: Rabbit, Porcine, Feline, Cynomolgus monkey, Dog, Bovine, Llama, Mink, Goat, Sheep

Packaging, Storage & Formulations

• Storage: Store at 4C in the dark.
• Buffer: PBS
• Preservative: No Preservative
• Purity: Protein G purified

Alternate Names for CD14 Antibody (TUK4) [HRP]

• CD14 antigen
• CD14 molecule
• CD14
• monocyte differentiation antigen CD14
• Myeloid cell-specific leucine-rich glycoprotein

Background

CD14 is a 53-55 kD glycosylphosphatidylinositol (GPI)-linked membrane glycoprotein also known as LPS receptor. CD14 is expressed at high levels on monocytes and macrophages, and at lower levels on granulocytes. Some dendritic cell populations such as interfollicular dendritic cells, reticular dendritic cells, and Langerhans cells have also been reported to express CD14. As a high-affinity receptor for LPS, CD14 is involved in the clearance of gram-negative pathogens and in the upregulation of adhesion molecules and cytokines expression in monocytes and neutrophils. The M5E2 antibody inhibits monocyte activation and cytokine production induced by LPS.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Bioinformatics

• Gene Symbol: CD14