CD14 Antibody (TUK4) - BSA Free

Flow Cytometry: CD14 Antibody (11-248.2) [NB100-62994] - Staining of human peripheral blood monocytes with Mouse anti Human CD14.

Flow Cytometry: CD14 Antibody (11-248.2) [NB100-62994] Staining of human peripheral blood monocytes with Mouse anti Human CD14

Flow Cytometry: CD14 Antibody (11-248.2) [NB100-62994] - Staining of human peripheral blood granulocytes with Mouse anti Human CD14.

Flow Cytometry: CD14 Antibody (11-248.2) [NB100-62994] - Staining of human peripheral blood monocytes probed with Mouse anti Human CD14.

Flow Cytometry: CD14 Antibody (11-248.2) [NB100-62994] - Staining of human peripheral blood monocytes with Mouse anti Human CD14.

Flow Cytometry: CD14 Antibody (11-248.2) [NB100-62994] - Staining of human peripheral blood monocytes with Mouse anti Human CD14.

Flow Cytometry: CD14 Antibody (11-248.2) [NB100-62994] - Staining of human peripheral blood monocytes with Mouse anti Human CD14.

Flow Cytometry: CD14 Antibody (11-248.2) [NB100-62994] - Staining of human peripheral blood monocytes with Mouse anti Human CD14.

• Reactivity: Hu
• Applications: ELISA, Flow, ICC/IF
• Clone: TUK4
• Clonality: Monoclonal
• Host: Mouse
• Conjugate: Unconjugated
• Format: BSA Free
• Concentration: 1 mg/ml

CD14 Antibody (TUK4) - BSA Free Summary

• Additional Information: Clone TUK4 was used by HLDA to establish CD designation.
• Immunogen: Made to Human CD14
• Localization: Cell Membrane
• Specificity: This product recognises the human CD14 cell surface antigen, expressed strongly by most peripheral blood monocytes and weakly on granulocytes. Clone Tuk4 has been shown to block SDF-induced chemotaxis of U937 cells in a dose-dependent manner(6).
• Isotype: IgG2a
• Clonality: Monoclonal
• Host: Mouse
• Gene: CD14
• Purity: Protein A purified

Applications/Dilutions

• Dilutions:
  • ELISA 1:100-1:2000
  • Flow Cytometry 1:50-1:200
  • Immunofluorescence
• Control:

  CD14 Overexpression Lysate

Reactivity Notes

Predicted cross-reactivities: Rabbit, Porcine, Feline, Cynomolgus monkey, Dog, Bovine, Llama, Mink, Goat, Sheep

Packaging, Storage & Formulations

• Storage: Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
• Buffer: PBS
• Preservative: 0.09% Sodium Azide
• Concentration: 1 mg/ml
• Purity: Protein A purified

Alternate Names for CD14 Antibody (TUK4) - BSA Free

• CD14 antigen
• CD14 molecule
• CD14
• monocyte differentiation antigen CD14
• Myeloid cell-specific leucine-rich glycoprotein

Background

CD14 is a single copy gene encoding 2 protein forms: a 50 to 55 kDa glycosylphosphatidylinositol anchored membrane protein (mCD14) and a monocyte or liver derived soluble serum protein (sCD14) that lacks the anchor. Both molecules are critical for lipopolysaccharide (LPS) dependent signal transduction, and sCD14 confers LPS sensitivity to cells lacking mCD14. Increased sCD14 levels are associated with inflammatory infectious diseases and high mortality in gram negative shock. CD14 also appears to be involved in clearance of gram-negative bacteria via its high affinity binding to LPS-LPB complexes.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Bioinformatics

• Gene Symbol: CD14
• Entrez:
  • 929(Human)
• Uniprot:
  • P08571()
  • P08571()