CD14 Antibody (Sa14-2) - Azide Free

Immunocytochemistry/Immunofluorescence: CD14 Antibody (Sa14-2) [NBP1-51121] - RAW264.7 cells were grown on coverslips, fixed with 1% parformaldehyde and blocked with BSA. As primary antibody was used at 2 ug/ml in PBS/BSA 3%.

Flow Cytometry: CD14 Antibody (Sa14-2) [NBP1-51121] - 100000 RAW264.7 cells were stained with 1ug/ml antibody for 1h at 4C (black - no staining; Red - isotype c ontrol; blue-NBP1-51121)

• Reactivity: Mu
• Applications: WB, Flow, ICC/IF
• Clone: Sa14-2
• Clonality: Monoclonal
• Host: Rat
• Conjugate: Unconjugated
• Format: Azide Free
• Concentration: 0.1 mg/ml

CD14 Antibody (Sa14-2) - Azide Free Summary

• Description: Novus Biologicals Rat CD14 Antibody (Sa14-2) - Azide Free (NBP1-51121) is a monoclonal antibody validated for use in WB, Flow and ICC/IF. All Novus Biologicals antibodies are covered by our 100% guarantee.
• Immunogen: The monoclonal antibody Sa14-2 reacts with the mouse monocyte marker CD14, a 53 kDa glycoprotein.
• Isotype: IgG2a
• Clonality: Monoclonal
• Host: Rat
• Gene: CD14
• Purity: Protein A or G purified

Applications/Dilutions

• Dilutions:
  • Flow Cytometry 1:10-1:1000
  • Immunocytochemistry/ Immunofluorescence 1:50
  • Western Blot
• Application Notes: Positive control: RAW264.7 cells

Packaging, Storage & Formulations

• Storage: Store at 4C.
• Buffer: 0.2 um filtered solution in PBS and 0.1% BSA
• Preservative: No Preservative
• Concentration: 0.1 mg/ml
• Purity: Protein A or G purified

Alternate Names for CD14 Antibody (Sa14-2) - Azide Free

• CD14 antigen
• CD14 molecule
• CD14
• monocyte differentiation antigen CD14
• Myeloid cell-specific leucine-rich glycoprotein

Background

The monoclonal antibody Sa14-2 reacts with the mouse monocyte marker CD14, a 53 kDa glycoprotein. CD14 is a glycophosphatidylinositol-linked protein, which is part of the LPS receptor complex. CD14 binds lipopolysaccharide (LPS) and as such, acts as a pattern recognition receptor (PRR). CD14 has also been suggested to mediate phagocytosis of bacteria an apoptotic cells. CD14 is involved in the endotoxin mediated release of Tumor Necrosis Factor-alpha by monocytic cells. CD14 is present on most monocytic and macrophage like cell types: monocytes, macrophages, Kupffer cells, pleural phagocytic cells and dendritic reticular cells. CD14 is present in low density on a subpopulation of granulocytes and activated or transformed B-cells.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Bioinformatics

• Gene Symbol: CD14