CD14 Antibody (50040) [Unconjugated]

• Reactivity: Hu
• Applications: ELISA(Cap)
• Clone: 50040
• Clonality: Monoclonal
• Host: Mouse
• Conjugate: Unconjugated

CD14 Antibody (50040) [Unconjugated] Summary

• Immunogen: Chinese hamster ovary cell line CHO-derived recombinant human CD14
  Thr20-Cys352
  Accession # P08571
• Specificity: Detects human CD14 in sandwich ELISAs.
• Source: N/A
• Isotype: IgG1
• Clonality: Monoclonal
• Host: Mouse
• Gene: CD14
• Purity Statement: Protein A or G purified from hybridoma culture supernatant

Applications/Dilutions

• Dilutions:
  • ELISA Capture (Matched Antibody Pair) 2-8 ug/mL
  • ELISA Detection (Matched Antibody Pair) 0.1-0.4 ug/mL
  • ELISA Standard (Matched Pair)
• Application Notes: ELISA Detection: Human CD14 Biotinylated Antibody (Catalog number BAF383)
  Standard: Recombinant Human CD14 (Catalog number 383-CD)

Packaging, Storage & Formulations

• Storage: Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.
• Buffer: Lyophilized from a 0.2 μm filtered solution in PBS and NaCl with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
• Preservative: No Preservative
• Reconstitution Instructions: Reconstitute at 0.5 mg/mL in sterile PBS.

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for CD14 Antibody (50040) [Unconjugated]

• CD14 antigen
• CD14 molecule
• CD14
• monocyte differentiation antigen CD14
• Myeloid cell-specific leucine-rich glycoprotein

Background

CD14 occurs both as a secreted protein and as a GPI-linked membrane protein. It plays a critical role in lipopolysaccharide (LPS)-dependent signal transduction.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Publications for CD14 Antibody (MAB3833)(4)

Publications using MAB3833

• WM Gama, CHM Frank, TVR Almeida, DS Dos Santos, YO Chaves, DF da Silva, PP Orlandi, FR Pereira, GF Magalhães, BJ Baptista, VL de Oliveir, AA da Silva B, MF Santana, RL Gonçalves, AG da Costa, MC Dos Santos, LC de Lima Fe, MVG Lacerda, PA Nogueira Immunologic biomarkers, morbidity and mortality among HIV patients hospitalised in a Tertiary Care Hospital in the Brazilian Amazon BMC infectious diseases, 2021-08-26;21(1):876. 2021-08-26 [PMID: 34445956] (ELISA Capture, Human)
• Komori M, Blake A, Greenwood M et al. Cerebrospinal fluid markers reveal intrathecal inflammation in progressive multiple sclerosis. Ann Neurol 7/1/2015 [PMID: 25808056]
• Ma C, Chang W, Shi G, Chang B, Cheng S, Shih Y, Wu H Recombinant thrombomodulin inhibits lipopolysaccharide-induced inflammatory response by blocking the functions of CD14. J Immunol, 2015-01-21;194(4):1905-15. 2015-01-21 [PMID: 25609841] (Binding Assay, Human)
• Gonzalez RM, Seurynck-Servoss SL, Crowley SA Development and validation of sandwich ELISA microarrays with minimal assay interference. J. Proteome Res., 2008-04-19;7(6):2406-14. 2008-04-19 [PMID: 18422355] (ELISA Microarray Development, Human)

Bioinformatics

• Gene Symbol: CD14
• Uniprot:
  • P08571()