CD14 Antibody (134620) [Phycoerythrin]

Human peripheral blood mononuclear cells (PBMCs) were stained with Mouse Anti-Human CD14 PE‑conjugated Monoclonal Antibody (Catalog # FAB3832P). View our protocol for Staining Membrane-associated Proteins.

• Reactivity: Hu
• Applications: Flow
• Clone: 134620
• Clonality: Monoclonal
• Host: Mouse
• Conjugate: Phycoerythrin

CD14 Antibody (134620) [Phycoerythrin] Summary

• Immunogen: Chinese hamster ovary cell line CHO-derived recombinant human CD14
  Thr20-Cys352
  Accession # P08571
• Specificity: Detects human CD14 in direct ELISAs and Western blots.
• Source: N/A
• Isotype: IgG1
• Clonality: Monoclonal
• Host: Mouse
• Gene: CD14
• Purity Statement: Protein A or G purified from hybridoma culture supernatant

Applications/Dilutions

• Dilutions:
  • Flow Cytometry 10 uL/10^6 cells

Packaging, Storage & Formulations

• Storage: Protect from light. Do not freeze.
  • 12 months from date of receipt, 2 to 8 °C as supplied.
• Buffer: Supplied in a saline solution containing BSA and Sodium Azide.
• Preservative: Sodium Azide

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for CD14 Antibody (134620) [Phycoerythrin]

• CD14 antigen
• CD14 molecule
• CD14
• monocyte differentiation antigen CD14
• Myeloid cell-specific leucine-rich glycoprotein

Background

CD14 is a 55 kDa cell surface glycoprotein that is preferentially expressed on monocytes/macrophages. The human CD14 cDNA encodes a 375 amino acid (aa) residue precursor protein with a 19 aa signal peptide and a C-terminal hydrophobic region characteristic for glycosylphosphatidyinositol (GPI)-anchored proteins. Human CD14 has four potential N-linked glycosylation sites and also bears O-linked carbohydrates. The amino acid sequence of human CD14 is approximately 65% identical with the mouse, rat, rabbit, and bovine proteins. CD14 is a pattern recognition receptor that binds lipopolysaccharides (LPS) and a variety of ligands derived from different microbial sources. The binding of CD14 with LPS is catalyzed by LPS-binding protein (LBP). The toll-like-receptors have also been implicated in the transduction of CD14-LPS signals. Similar to other GPI-anchored proteins, soluble CD14 can be released from the cell surface by phosphatidyinositol-specific phospholipase C. Soluble CD14 has been detected in serum and body fluids. High concentrations of soluble CD14 have been shown to inhibit LPS-mediated responses. However, soluble CD14 can also potentiate LPS response in cells that do not express cell surface CD14.

1. Wright, S.D. et al. (1990) Science 249:1431.
2. Pugin, J. et al. (1993) Proc. Natl. Acad. Sci. USA 90:2744.
3. Beutler, B. (2000) Current Opinion in Immunology 12:20.
4. Stelter, F. (2000) Chem. Immunol. 74:25.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Publications for CD14 Antibody (FAB3832P)(14)

Publications using FAB3832P

• S Sbrana, J Campolo, A Clemente, L Bastiani, A Cecchettin, E Ceccherini, C Caselli, D Neglia, O Parodi, D Chiappino, JM Smit, AJ Scholte, G Pelosi, S Rocchiccio Blood Monocyte Phenotype Fingerprint of Stable Coronary Artery Disease: A Cross-Sectional Substudy of SMARTool Clinical Trial Biomed Res Int, 2020-07-27;2020(0):8748934. 2020-07-27 [PMID: 32802883] (Flow Cytometry, Human)
• B Bauvois, E Pramil, L Jondrevill, E Chapiro, C Quiney, K Maloum, SA Susin, F Nguyen-Kha Relation of Neutrophil Gelatinase-Associated Lipocalin Overexpression to the Resistance to Apoptosis of Tumor B Cells in Chronic Lymphocytic Leukemia Cancers (Basel), 2020-07-31;12(8):. 2020-07-31 [PMID: 32751884] (Flow Cytometry, Human)
• David Heinzmann, Stefan Fu beta , Saskia v. Ungern-Sternberg, Jürgen Schreieck, Meinrad Gawaz, Michael Gramlich et al. TGF beta Is Specifically Upregulated on Circulating CD14++ CD16+ and CD14+ CD16++ Monocytes in Patients with Atrial Fibrillation and Severe Atrial Fibrosis Cellular Physiology and Biochemistry 2018-04-29 [PMID: 30153670]
• Z Agod, K Pazmandi, D Bencze, G Vereb, T Biro, A Szabo, E Rajnavolgy, A Bacsi, P Engel, A Lanyi Signaling Lymphocyte Activation Molecule Family 5 Enhances Autophagy and Fine-Tunes Cytokine Response in Monocyte-Derived Dendritic CellsStabilization of Interferon Regulatory Factor 8 Front Immunol, 2018-01-26;9(0):62. 2018-01-26 [PMID: 29434592] (Flow Cytometry, Human)
• H Fayyad-Kaz, M Fayyad-Kaz, B Badran, D Bron, L Lagneaux, M Najar Study of the microRNA expression profile of foreskin derived mesenchymal stromal cells following inflammation priming J Transl Med, 2017-01-13;15(1):10. 2017-01-13 [PMID: 28086811] (Flow Cytometry, Human)
• TLR4/CD14 Variants-Related Serologic and Immunologic Dys-Regulations Predict Severe Sepsis in Febrile De-Compensated Cirrhotic Patients PLoS ONE, 2016-11-18;11(11):e0166458. 2016-11-18 [PMID: 27861595] (Flow Cytometry, Human)
• Anthony J. Hesketh, Caroline Maloney, Christopher A. Behr, Morris C. Edelman, Richard D. Glick, Yousef Al-Abed et al. The Macrophage Inhibitor CNI-1493 Blocks Metastasis in a Mouse Model of Ewing Sarcoma through Inhibition of Extravasation PLOS ONE 2015-12-28 [PMID: 26709919]
• ZHENXIN WANG, JIANFENG YANG, YANBO ZHU, YUN ZHU, BIN ZHANG, YINGHUI ZHOU Differential expression of 2IgB7-H3 and 4IgB7-H3 in cancer cell lines and glioma tissues Oncology Letters 2015-10-01 [PMID: 26622819]
• Engin Baturcam, Jehad Abubaker, Ali Tiss, Mohamed Abu-Farha, Abdelkrim Khadir, Fahad Al-Ghimlas et al. Physical Exercise Reduces the Expression of RANTES and Its CCR5 Receptor in the Adipose Tissue of Obese Humans Mediators of Inflammation 2014-04-17 [PMID: 24895488]
• CXCR4 positive and angiogenic monocytes in myocardial infarction. Thromb Haemost, 2012-12-06;109(2):255-62. 2012-12-06 [PMID: 23223950] (Flow Cytometry, Human)
• Albert R, Vereb Z, Csomos K, Moe M, Johnsen E, Olstad O, Nicolaissen B, Rajnavolgyi E, Fesus L, Berta A, Petrovski G Cultivation and characterization of cornea limbal epithelial stem cells on lens capsule in animal material-free medium. PLoS ONE, 2012-10-09;7(10):e47187. 2012-10-09 [PMID: 23056608] (Flow Cytometry, Human)
• Pilz GA, Braun J, Ulrich C, Felka T, Warstat K, Ruh M, Schewe B, Abele H, Larbi A, Aicher WK Human mesenchymal stromal cells express CD14 cross-reactive epitopes. Cytometry A, 2011-07-06;79(8):635-45. 2011-07-06 [PMID: 21735544] (Flow Cytometry, Human)
• McAulay KA, Haque T, Urquhart G, Bellamy C, Guiretti D, Crawford DH Epitope specificity and clonality of EBV-specific CTLs used to treat posttransplant lymphoproliferative disease. J. Immunol., 2009-03-15;182(6):3892-901. 2009-03-15 [PMID: 19265169] (Flow Cytometry, Human)
• Ahmad R, Ennaciri J, Cordeiro P, El Bassam S, Menezes J Herpes simplex virus-1 up-regulates IL-15 gene expression in monocytic cells through the activation of protein tyrosine kinase and PKC zeta/lambda signaling pathways. J. Mol. Biol., 2006-12-28;367(1):25-35. 2006-12-28 [PMID: 17239392] (Flow Cytometry, Human)

Bioinformatics

• Gene Symbol: CD14
• Uniprot:
  • P08571()