Recombinant Human CD-M6PR His Protein

SDS-Page: Recombinant Human CD-M6PR Protein [NBP2-51688] - 15% SDS Page (3 ug)

• Reactivity: Hu
• Applications: PAGE
• Concentration: 0.5 mg/ml

Recombinant Human CD-M6PR His Protein Summary

• Description: A recombinant protein with a N-Terminal His-tag and corresponding to the amino acids 27-185 of Human CD-M6PR
  
  

  Source: E.coli

  Amino Acid Sequence: MGSSHHHHHH SSGLVPRGSH MGSTEEKTCD LVGEKGKESE KELALVKRLK PLFNKSFEST VGQGSDTYIY IFRVCREAGN HTSGAGLVQI NKSNGKETVV GRLNETHIFN GSNWIMLIYK GGDEYDNHCG KEQRRAVVMI SCNRHTLADN FNPVSEERGK VQDCFYLFEM DSSLACSPEI SH

• Source: E. coli
• Protein/Peptide Type: Recombinant Protein
• Gene: M6PR
• Purity: >85%, by SDS-PAGE

Applications/Dilutions

• Dilutions:
  • SDS-Page
• Theoretical MW: 20.3 kDa.
  Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Packaging, Storage & Formulations

• Storage: Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
• Buffer: 20 mM Tris-HCl buffer (pH 8.0), 0.15 M NaCl, 10% glycerol, 1 mM DTT
• Preservative: No Preservative
• Concentration: 0.5 mg/ml
• Purity: >85%, by SDS-PAGE

Alternate Names for Recombinant Human CD-M6PR His Protein

• 000 Man6PR
• 46 kDa mannose 6-phosphate receptor
• 46-kDa mannose 6-phosphate receptor
• cation-dependent mannose-6-phosphate receptor
• CD Man-6-P receptor
• CDM6PR
• CD-M6PR
• CD-MPR
• FLJ32994
• M6PR
• mannose-6-phosphate receptor (cation dependent)
• MPR 46
• MPR46
• MPRD
• Mr 46
• SMPR

Background

Lysosomal enzymes containing one or two mannose 6-phosphate (man6P) moieties are moved about in the cell by two distinct but interconnected cycles by means of 300 kDa cation independent mannose 6-phospate receptors (CI-MPR). The biosynthetic cycle refers to the MPR transport of newly synthesized lysosomal enzymes from the trans Golgi network to late endosomes or early lysosomes. The endocytic cycle transports extracellular lysosomal enzymes from the plasma membrane via clathrin-coated vesicles to early endosomes. The entire pool of MPRs cycles between these cellular compartments every 3 hours. The steady state distribution of MPR's is predominantly within late endosomes, fewer in the trans Golgi network and ~10 % at cell surface. In addition to its man6P binding activity, the MPR contains a separate binding site for the type II insulin-like growth factor and is capable of binding both man6P and IGF-II simultaneously. An ~240 kDa soluble, truncated form, representing the extracellular domain of the protein, has also been found circulating in serum and is capable of binding both ligands.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Peptides and proteins are guaranteed for 3 months from date of receipt.

Publications for CD-M6PR Recombinant Protein (NBP2-51688)(1)

Publication using NBP2-51688

• Li, D;Hao, X;Dong, Y;Zhang, M;Song, Y; PF4V1, an miRNA-875-3p target, suppresses cell proliferation, migration, and invasion in prostate cancer and serves as a potential prognostic biomarker Cancer Manag Res 2019-03-21 [PMID: 30962718] (In Vivo, Mouse)

Bioinformatics

• Gene Symbol: M6PR