CD-M6PR Antibody

Product Discontinued

CD-M6PR Antibody Summary

• Immunogen: Peptide with sequence KGGDEYDNHCGKEQ corresponding to internal region according to NP_002346.1.
• Marker: Late Endosome Marker
• Isotype: IgG
• Clonality: Polyclonal
• Host: Goat
• Gene: M6PR
• Purity: Immunogen affinity purified

Applications/Dilutions

• Dilutions:
  • Peptide ELISA Detection limit 1:4000
  • Western Blot
• Application Notes: WB: Preliminary experiments gave bands at approx. 70 kDa band and 35 kDa band in human lymph node and thyroid gland lysates after 1 ug/ml antibody staining. Please note that currently we cannot find an explanation in the literature for the band we observe given the calculated size of 31.0 kDa band according to NP_002346.1. Both detected bands were successfully blocked by incubation with the immunizing peptide (and BLAST results with the immunizing peptide sequence did not identify any other proteins to explain the additional bands). Use in Western blot reported in scientific literature (PMID: 22983091).

Packaging, Storage & Formulations

• Storage: Store at -20C. Avoid freeze-thaw cycles.
• Buffer: Tris saline (20 mM Tris pH 7.3, 150 mM NaCl), 0.5% BSA
• Preservative: 0.02% Sodium Azide
• Concentration: 0.5 mg/ml
• Purity: Immunogen affinity purified

Notes

Please note, this antibody is considered Innovators Grade. Innovators Grade antibodies are generally unvalidated and require additional characterization for most new species/applications. Novus has made these antibodies available through our Innovators Reward program. Complete an online review with image, detailing your positive or negative results. In return, you receive a discount voucher for 100% of the purchase price of the reviewed product. Please contact us at innovators@novusbio.com for more details.

Alternate Names for CD-M6PR Antibody

• 000 Man6PR
• 46 kDa mannose 6-phosphate receptor
• 46-kDa mannose 6-phosphate receptor
• cation-dependent mannose-6-phosphate receptor
• CD Man-6-P receptor
• CDM6PR
• CD-M6PR
• CD-MPR
• FLJ32994
• M6PR
• mannose-6-phosphate receptor (cation dependent)
• MPR 46
• MPR46
• MPRD
• Mr 46
• SMPR

Background

The protein encoded by this gene is a receptor for mannose-6-phosphate groups on lysosomal enzymes. The receptor forms a homodimer or homotetramer for intracellular targeting of lysosomal enzymes and export of newly synthesized lysosomal enzymes into the cell secretions. The receptor is an integral membrane protein which localizes to the trans-Golgi reticulum, endosomes, and the plasma membrane.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Publications for CD-M6PR Antibody (NB100-93413)(2)

Publications using NB100-93413

• Frei AP, Jeon OY, Kilcher S et al. Direct identification of ligand-receptor interactions on living cells and tissues Nat Biotechnol 2012-09-16 [PMID: 22983091] (WB, Human)
• Zygmunt M, McKinnon T, Herr F et al. HCG increases trophoblast migration in vitro via the insulin-like growth factor-II/mannose-6 phosphate receptor. Mol Hum Reprod 2005-04-01 [PMID: 15749784]

Bioinformatics

• Gene Symbol: M6PR
• Entrez:
  • 4074(Human)
  • 17113(Mouse)
  • 312689(Rat)
• Uniprot:
  • P20645()