CCRL2/CRAM-A/B Antibody (BZ2E3) [DyLight 488]

• Reactivity: Mu
• Applications: WB, ELISA, Flow, ICC/IF, IHC, IP, Func
• Clone: BZ2E3
• Clonality: Monoclonal
• Host: Rabbit
• Conjugate: DyLight 488

CCRL2/CRAM-A/B Antibody (BZ2E3) [DyLight 488] Summary

• Description: This conjugate is made on demand and is not held in inventory. Each order represents a unique lot. All conjugates are generated using validated conjugation protocols and meet strict degree of labeling (F/P) specifications; additional information is available upon request. The antibody concentration is provided on the individual product label.
  
  For specialized conjugation needs, like large quantities, specific concentrations, and defined F/P ratios, bulk and custom antibody conjugation services are available.
• Additional Information: Recombinant Monoclonal Antibody
• Immunogen: This antibody was raised by immunising Wistar Furth rats with the mCCRL2/CRAM-A/B peptide/KLH conjugate. The immunizing amino-terminal mCCRL2/CRAM-A/B peptide with the sequence NH2-MDNYTVAPDDEYDVLILDDYLDNSC-COOH, corresponding to residues 1-24 of mCCRL2/CRAM-A/B, with a nonnative carboxyl-terminal cysteine to facilitate conjugation to keyhole limpet hemocyanin (KLH).
• Specificity: This antibody is specific for murine CCRL2/CRAM-A/B (formerly known as L-CCR), which is expressed on mast cells, activated macrophages, and endothelial cells. This receptor binds the leukocyte chemoattractant chemerin without triggering classical GPCR signaling, providing a specific mechanism for local enrichment of chemerin at inflammatory sites. CCRL2/CRAM-A/B presents active chemerin to leukocytes expressing the chemoattractant receptor CMKLR1/Chemr23. Chemerin can block anti-CCRL2/CRAM-A/B antibody binding.
• Isotype: IgG Kappa
• Clonality: Monoclonal
• Host: Rabbit
• Gene: CCRL2
• Purity: Protein A purified

Applications/Dilutions

• Dilutions:
  • ELISA
  • Flow Cytometry
  • Functional Assay
  • Immunocytochemistry/ Immunofluorescence
  • Immunohistochemistry
  • Immunoprecipitation
  • Western Blot
• Application Notes: Recommended applications are based on validated applications from the unconjugated base product NBP3-11982. This conjugated antibody is not kept in inventory and is made to order using validated conjugation protocols.

Packaging, Storage & Formulations

• Storage: Store at 4C in the dark.
• Buffer: 50mM Sodium Borate
• Preservative: 0.05% Sodium Azide
• Purity: Protein A purified

Notes

DyLight (R) is a trademark of Thermo Fisher Scientific Inc. and its subsidiaries.

Alternate Names for CCRL2/CRAM-A/B Antibody (BZ2E3) [DyLight 488]

• ACKR5
• C-C chemokine receptor-like 2
• CCR11
• CCR6
• CCRL2
• CCRL2/CRAM-A/B
• chemokine (C-C motif) receptor-like 2
• Chemokine receptor CCR11
• Chemokine receptor X
• CKRX
• CKRXMGC116710
• CRAM
• CRAMA
• CRAM-A
• CRAMB
• CRAM-B
• FLJ55815
• HCR
• HCRMGC34104
• Putative MCP-1 chemokine receptor

Background

CCRL2 encodes a chemokine receptor like protein, which is predicted to be a seven transmembrane protein and most closely related to CCR1. Chemokines and their receptors mediated signal transduction are critical for the recruitment of effector immune cells to the site of inflammation. This gene is expressed at high levels in primary neutrophils and primary monocytes, and is further upregulated on neutrophil activation and during monocyte to macrophage differentiation. The function of this gene is unknown. This gene is mapped to the region where the chemokine receptor gene cluster is located. [provided by RefSeq]

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Bioinformatics

• Gene Symbol: CCRL2